Additional file 1: of Text message-based diabetes self-management support (SMS4BG): study protocol for a randomised controlled trial

List of Behaviour Change Techniques (BCT) utilised in SMS4BG by grouping (BCT Taxonomy v1). (PDF 67 kb

The Effects of JNK and NF-κB Inhibition on Visfatin Protein Expression in Human Adipocytes.

<p>This figure shows the effect of JNK and NF-κB blockade, alone and in combination, on the mean relative fold protein expression (±SEM) of visfatin in isolated Abd Sc adipocytes compared with control (untreated adipocytes), given an arbitrary value of one. The representative Western blots are shown above (n = 4, p-values: p<0.05*, p<0.01**).</p

A–E. Photographs of Immunological Staining of Visfatin Protein.

<p>This figure shows the immunological staining of visfatin in (A) human kidney negative (primary antibody omitted); (B) human kidney positive control; (C) human AT PBEF negative (primary antibody omitted); (D) human Om AT; (E) human Abd Sc AT. Red arrows highlight areas of red staining to show the presence of the visfatin protein.</p

Clinical and Biochemical Characteristics of T2DM Subjects Pre and Post 10 week Rosiglitazone Treatment.

<p>Data are presented as mean (±SD). Non-parametric data have been log transformed in which case they are presented as mean (interquartile range).</p

Clinical and Biochemical Characteristics of T2DM Subjects Compared with Control Subjects.

<p>Data are presented as mean (±SD), unless otherwise stated. Non-parametric data have been log transformed in which case they are presented as mean (interquartile range).</p

Visfatin Protein Expression in Relation to Adiposity and Depot Specificity.

<p>This figure shows the mean relative fold protein expression of visfatin in omental (Om) versus abdominal (Abd) adipose tissue depots in matched pairs, from a cohort of lean and obese subjects, where the Abd Sc depot has been standardized. The representative Western blots are shown above. Lean samples: n = 7 & Obese samples: n = 6; p-values: p<0.001.</p

A&B. The Effect of Insulin Alone and in Combination with RSG on Visfatin Protein Expression in Isolated Adipocytes.

<p>These figures show the mean relative protein expression (±SEM) of visfatin in isolated human adipocytes treated with insulin (1 nM and 100 nM, respectively, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020287#pone-0020287-g003" target="_blank">Figure 3A</a>) and insulin in combination with RSG (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020287#pone-0020287-g003" target="_blank">Figure 3B</a>), compared with control (untreated adipocytes) as an arbitrary value of 1. The representative Western blots are shown above (n = 4, p-values: p<0.05*, p<0.01**).</p

A&B. The Effects of Insulin and RSG on IKKβ and JNK1/2 Protein Expression.

<p>These figures shows the effect of insulin, alone (100 nM), and insulin in combination with RSG (Ins 100 nM/RSG 10 nM) on the mean relative fold protein expression (± SEM) of IKKβ (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020287#pone-0020287-g004" target="_blank">Figure 4A</a>) and JNK 1/2 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020287#pone-0020287-g004" target="_blank">Figure 4B</a>). The representative Western blots are shown above (n = 4, p-values: p<0.05*, p<0.001***).</p