1 research outputs found
Development of duplex RT-PCR for detection of <i>Konjac mosaic virus</i> and Spathiphyllum chlorotic vein banding virus in taro and peace lily
120-122Konjac mosaic virus (KoMV) and Spathiphyllum
chlorotic vein banding virus (SCVbV) infecting aroids in Andhra Pradesh,
India have recently been reported as distinct potyviruses. Primers were
designed based on their partial genome sequence (GenBank A/C EU979524, GQ421462) to detect above
viruses by
duplex-reverse transcription-polymerase chain reaction (duplex RT-PCR). Using
this method, the two viruses were individually detected in limited number of
filed/nursery collected Colocasia esculenta (taro) and Spathiphyllum
spp. (peace lily). No
co-infections with two viruses were traced among the screened samples. However,
they were simultaneously detected in the simulated leaf samples of taro and
peace lily (1:1, w/w).
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