Relationship between respiratory response and metabolic pathways of S. Derby and S. Mbandaka.

<p>Respiratory responses which distinguish <i>S</i>. Derby and <i>S</i>. Mbandaka under aerobic conditions at 25°C and the corresponding metabolic pathway for the metabolism of the compound from uptake to glycolysis. Only <i>S</i>. Mbandaka respires on D-galactonic acid-g-lactone (DGL) (a), this can be attributed to the lack of pathway genes (e) (KEGG map 00052, created 31/5/12). Likewise, <i>S</i>. Derby is unable to respire on D-glucosaminic acid (DGA) while <i>S</i>. Mbandaka can (c) yet both serovars possess the same pathway genes (g) (KEGG map 00030, created 9/3/13). For both DGL and DGA there were gaps in the SEEDmodel reconstruction which prevented the metabolism of the compounds, these were filled using ECBLAST and SEEDviewer BLASTp (yellow stars). Both serovars contain the same pathway genes for the metabolism of mucic acid (f) (KEGG map 00053, created 28/9/09) yet the area under the respiratory curve for this compound, and not the log phase gradient or lag phase, was significantly greater for <i>S</i>. Derby (b).</p

Clinical details for English pig herds with outbreaks of septicaemia due to <i>Kpp</i> infection.

<p>Clinical details for English pig herds with outbreaks of septicaemia due to <i>Kpp</i> infection.</p

Frequency of gross lesions in thirty five piglets which died of septicaemia due to <i>Klebsiella pneumoniae</i>.

<p>Frequency of gross lesions in thirty five piglets which died of septicaemia due to <i>Klebsiella pneumoniae</i>.</p

Comparison of virulence determinants in ST25 <i>Klebsiella pneumoniae</i> isolates to other <i>K</i>. <i>pneumoniae</i> from porcine cases.

<p>Comparison of virulence determinants in ST25 <i>Klebsiella pneumoniae</i> isolates to other <i>K</i>. <i>pneumoniae</i> from porcine cases.</p

BRIG analysis: Location of genomic regions unique to ST25 case isolates.

<p>The 3 phage sequences linked to <i>Kpp</i> ST25 isolates are highlighted with red bars. C, case; CR, case-related; cnda, contemporary non disease associated; H, historical.</p

Clinical interventions and efficacy following the diagnosis of piglet septicaemia due to <i>Kpp</i>.

<p>Clinical interventions and efficacy following the diagnosis of piglet septicaemia due to <i>Kpp</i>.</p

Emergence of <i>Klebsiella pneumoniae</i> subspecies <i>pneumoniae</i> as a cause of septicaemia in pigs in England - Fig 4

<p><b>(A) Gel picture showing electrophoretic separation of plasmids carried by 16 <i>Kpp</i> isolates</b>, migrating in 0.8% agarose (TBE), for 270 min at 150 v, 4°C, are shown. Yellow stars indicate location of pKPMC25 common to <i>Kpp</i> ST25 isolates. The plasmids in lane 1 and 21 are supercoiled DNA ladder. Lane 2, 12, 20 shows the reference plasmid bands from strain 39R861 (148 kb, 63 kb, 36 kb, genomic DNA band and 6.8 kb. Lane 3, 8–10 and 16–18 are <i>Kpp</i> ST25 isolates. Lanes 4–7, 11, 13–15 and 19 are non-ST25 <i>Kpp</i> isolates. (<b>B). Overview of pKPMC25 plasmid sequence features and comparison to closest matching plasmids</b>.</p

Results of <i>Kpp</i> multilocus sequence typing for British historical (1993–2010) isolates, porcine contemporary non-disease associated (CNDA) isolates collected 2011 to 2014 and for outbreak cases (C) and case-related but non cases (CR).

<p>Results of <i>Kpp</i> multilocus sequence typing for British historical (1993–2010) isolates, porcine contemporary non-disease associated (CNDA) isolates collected 2011 to 2014 and for outbreak cases (C) and case-related but non cases (CR).</p

Phylogenetic SNP tree analysis of <i>Klebsiella</i> genomes, using <i>Klebsiella pneumoniae</i> NTUH-K2044 as reference genome.

<p>Shaded isolates the ST25 case isolates. There were a total of 195803 SNP positions in the final dataset. Node colours indicate bootstrap support according to figure legend (Blue = low bootstrap support, Red = high bootstrap support).</p

Virulence gene analysis of 51 isolates from 1993 to 2011.

<p>Virulence gene analysis of 51 isolates from 1993 to 2011.</p