2011 German Escherichia coli outbreak: Alignment-free whole-genome phylogeny by feature frequency profiles

Accuracy of SNP-based whole-genome phylogeny reconstruction relies heavily on quality of sequence alignment which is particularly hindered by poorly assembled genomes. Alignment-free methods might provide additional insights. Here, we constructed a whole-genome phylogeny of 9 E.coli isolates from the 2011 German outbreak against existing E. coli genomes using the alignment-free feature frequency profile method. In addition, we looked for gene elements that distinguish the outbreak group from the other E. coli strains and possibly accounted for the emergence of the outbreak isolates using the distinguishing feature analysis

Building Trust in Online Shopping: The Moderating Effect of Individualism and Collectivism

As online shopping becoming more global, consumers with different culture had different consideration when shopping online. Thus, this study attempts to investigate how one specific dimension of culture, individualism-collectivism, moderates the relationship between online trust and its determinants The determinant factors under investigation include perceived risk, privacy, security, social exchange and information content. Data collected from customers of online shops from Hong Kong and Switzerland were used to test the research model. The result indicated that perceived risk had a negative relationship with Trust. Perceived risk had a stronger effect on trust for collectivists than individualists. Also, Security had a positive relationship with trust. The study showed that information content was positively related to trust of online vendors. Information content had a stronger effect on trust for individualists then collectivists

Effects Of Supply Chain Strategies And Practices On Firm Performance

A number of studies have looked at the impacts of supply chain strategies on firm performance. Yet, how different supply chain practices translate to firm performance remains unclear. Drawing from the resource based view, this study investigates the role of supply chain capabilities in mediating the relationships between supply chain practices and firm performance. A survey was conducted with 171 manufacturing firms in five Chinese cities to empirically validate the research model. Our findings suggest that, supply chain practices, as driven by supply chain strategies, create different supply chain capabilities and lead to firm performance. The findings shed some lights on the mechanism in which supply chain strategies and practices translate into firm performance

2011 German Escherichia coli O104:H4 outbreak: whole-genome phylogeny without alignment

<p>Abstract</p> <p>Background</p> <p>A large-scale <it>Escherichia coli </it>O104:H4 outbreak occurred in Germany from May to July 2011, causing numerous cases of hemolytic-uremic syndrome (HUS) and deaths. Genomes of ten outbreak isolates and a historical O104:H4 strain isolated in 2001 were sequenced using different new generation sequencing platforms. Phylogenetic analyses were performed using various approaches which either are not genome-wide or may be subject to errors due to poor sequence alignment. Also, detailed pathogenicity analyses on the 2001 strain were not available.</p> <p>Findings</p> <p>We reconstructed the phylogeny of <it>E. coli </it>using the genome-wide and alignment-free feature frequency profile method and revealed the 2001 strain to be the closest relative to the 2011 outbreak strain among all available <it>E. coli </it>strains at present and confirmed findings from previous alignment-based phylogenetic studies that the HUS-causing O104:H4 strains are more closely related to typical enteroaggregative <it>E. coli </it>(EAEC) than to enterohemorrhagic <it>E. coli</it>. Detailed re-examination of pathogenicity-related virulence factors and secreted proteins showed that the 2001 strain possesses virulence factors shared between typical EAEC and the 2011 outbreak strain.</p> <p>Conclusions</p> <p>Our study represents the first attempt to elucidate the whole-genome phylogeny of the 2011 German outbreak using an alignment-free method, and suggested a direct line of ancestry leading from a putative EAEC-like ancestor through the 2001 strain to the 2011 outbreak strain.</p

2011 German Escherichia coli outbreak: Prophage analysis of close-assembled TY2482 against 55989 using PHAST

Using the Hiseq data of the German E. coli outbreak isolate TY2482, preliminary prophage analyses have been performed by some researchers previously. With the closed assembly of the same isolate being available, another round of analysis might help in resolving questions that remain unclear due to the incompleteness of the dataset

UNDERSTANDING THE DETERMINANTS OF BLOCKCHAIN ADOPTION IN SUPPLY CHAINS: AN EMPIRICAL STUDY IN CHINA

This study adopts affordance approach to understand how supply chain managers interpret the possible benefits that can be performed within blockchain-enabled supply chain applications. With a focus on governance efficiency improvement, the impacts of traceability and transparency affordance on uncertainty reduction were examined from the perspective of transaction cost theory in supply chain industry. Partial least squares – structural equation modelling (PLS-SEM) was used to analyze the data collected from 364 experienced supply chain managers in China. The results revealed that traceability affordance exerted significant impacts on environmental and trading partner uncertainty rather than transparency affordance, which in turn affected the adoption intention. This study contributes to the extant literature by embedding transaction cost in blockchain affordances. The findings are useful in guiding practitioners to improve blockchain system design for reducing uncertainties in supply chain environment, leading to a higher adoption rate of blockchain technology

養龍神話的魔幻化重寫 : 讀也斯 \u3c養龍人師門\u3e

在香港，也斯被評為可能是最早譯介當代拉丁美洲小說的譯者，也可能是最早發表帶魔幻色彩的小說的作者。〈養龍人師門〉帶有濃厚的魔幻寫實主義的色彩，業界自然不乏以魔幻寫實主義分析也斯作品，然而似乎只把魔幻寫實視為創作技法，片面理解魔幻寫實主義，忽略其背後的精神。本文嘗試結合學者研究魔幻寫實主義的成果，旁借也斯訪談錄，分析也斯在〈養龍人師門〉如何用魔幻寫實主義重寫神話。本文核心主要是環繞以下兩個問題： 1. 也斯為養龍神話加入／重寫了不少情節和細節，這些故事新質有何意義？ 2. 也斯如何以魔幻手法處理重寫，並體驗了怎樣的魔幻寫實主義的特色？ 為行文方便，本文將「受魔幻寫實主義影響」、「經過魔幻寫實主義的手 法處理」縮寫成「魔幻化」

Do people feel they belong? Socio‐political factors shaping the place attachment of Hong Kong citizens

Hong Kong citizens’ sense of belonging has gone through a period of fluctuation during the period of rapid socio‐political and legal change since the outbreak of the Anti‐Extradition Law Amendment Bill Movement in 2019. This study explored how multiple dimensions of the place attachment of Hong Kong citizens have been shaped by factors associated with these changes. Six socio‐political variables were incorporated into the three dimensions of the person–process–place (PPP) framework. Based on a representative survey of the local population (n = 768), we found that political inclination and identity were significantly associated with the sense of place, with citizens identifying as Chinese and aligning with the pro‐establishment camp showing higher levels of place attachment. Mobility was negatively associated with place attachment, whereas the correlation between attachment and perceptions of the law and legal system was positive. The study has implications for Hong Kong’s current socio‐political and institutional environment and for emigration. It also demonstrates the wider applicability of the PPP framework for identifying and clarifying the various predictors of different dimensions of place attachment

The Enhanced metastatic potential of hepatocellular carcinoma (HCC) cells with sorafenib resistance

Acquired resistance towards sorafenib treatment was found in HCC patients, which results in poor prognosis. To investigate the enhanced metastatic potential of sorafenib resistance cells, sorafenib-resistant (SorR) cell lines were established by long-term exposure of the HCC cells to the maximum tolerated dose of sorafenib. Cell proliferation assay and qPCR of ABC transporter genes (ABCC1-3) were first performed to confirm the resistance of cells. Migration and invasion assays, and immunoblotting analysis on the expression of epithelial to mesenchymal transition (EMT) regulatory proteins were performed to study the metastatic potential of SorR cells. The expression of CD44 and CD133 were studied by flow cytometry and the gene expressions of pluripotency factors were studied by qPCR to demonstrate the enrichment of cancer stem cells (CSCs) in SorR cells. Control (CTL) and SorR cells were also injected orthotopically to the livers of NOD-SCID mice to investigate the development of lung metastasis. Increased expressions of ABCC1-3 were found in SorR cells. Enhanced migratory and invasive abilities of SorR cells were observed. The changes in expression of EMT regulatory proteins demonstrated an activation of the EMT process in SorR cells. Enriched proportion of CD44+ and CD44+CD133 + cells were also observed in SorR cells. All (8/8) mice injected with SorR cells demonstrated lung metastasis whereas only 1/8 mouse injected with CTL cells showed lung metastasis. HCC cells with sorafenib resistance demonstrated a higher metastatic potential, which may be due to the activated EMT process. Enriched CSCs were also demonstrated in the sorafenib resistant cells. This study suggests that advanced HCC patients with acquired sorafenib resistance may have enhanced tumor growth or distant metastasis, which raises the concern of long-term sorafenib treatment in advanced HCC patients who have developed resistance of sorafenib. © 2013 Chow et al.published_or_final_versio

The prevalence and factors for cancer screening behavior among people with severe mental illness in Hong Kong

Objectives: Screening is useful in reducing cancer incidence and mortality. People with severe mental illness (PSMI) are vulnerable to cancer as they are exposed to higher levels of cancer risks. Little is known about PSMI's cancer screening behavior and associated factors. The present study examined the utilization of breast, cervical, prostate, and colorectal cancer screening among PSMI in Hong Kong and to identify factors associated with their screening behaviors. Method: 591 PSMI from community mental health services completed a cross-sectional survey. Results: The percentage of cancer screening behavior among those who met the criteria for particular screening recommendation was as follows: 20.8% for mammography; 36.5% for clinical breast examination (CBE); 40.5% for pap-smear test; 12.8% for prostate examination; and 21.6% for colorectal cancer screening. Results from logistic regression analyses showed that marital status was a significant factor for mammography, CBE, and pap-smear test; belief that cancer can be healed if found early was a significant factor for pap-smear test and colorectal screening; belief that one can have cancer without having symptoms was a significant factor for CBE and pap-smear test; belief that one will have a higher risk if a family member has had cancer was a significant factor for CBE; and self-efficacy was a significant factor for CBE and papsmear test behavior. Conclusions: Cancer screening utilization among PSMI in Hong Kong is low. Beliefs about cancer and self-efficacy are associated with cancer screening behavior. Health care professionals should improve the knowledge and remove the misconceptions about cancer among PSMI; self-efficacy should also be promoted