Mammalian Cells

Cell-type specific gene expression programs are tightly linked to epigenetic modifications on DNA and histone proteins. Here, we used a novel CRISPR-based epigenome editing approach to control gene expression spatially and temporally. We show that targeting dCas9-p300 complex to distal non-regulatory genomic regions reprograms the chromatin state of these regions into enhancer-like elements. Notably, through controlling the spatial distance of these induced enhancers (i-Enhancer) to the promoter, the gene expression amplitude can be tightly regulated. To better control the temporal persistence of induced gene expression, we integrated the auxin-inducible degron technology with CRISPR tools. This approach allows rapid depletion of the dCas9-fused epigenome modifier complex from the target site and enables temporal control over gene expression regulation. Using this tool, we investigated the temporal persistence of a locally edited epigenetic mark and its functional consequences. The tools and approaches presented here will allow novel insights into the mechanism of epigenetic memory and gene regulation from distal regulatory sites. (C) 2018 Published by Elsevier Ltd.C1 [Kuscu, Cem; Mammeadov, Rashad; Czikora, Agnes; Tufan, Turan; Fischer, Natasha Lopes; Bekiranov, Stefan; Adli, Mazhar] Univ Virginia, Sch Med, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA.[Arslan, Sevki] Pamukkale Univ, Dept Biol, TR-20160 Denizli, Turkey.[Kanemaki, Masato] Natl Inst Genet, Mishima, Sizuoka 4118540, Japan.[Kanemaki, Masato] SOKENDAI, Mishima, Sizuoka 4118540, Japan.[Kanemaki, Masato] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan.[Unlu, Hayrunnisa] Ankara Univ, Sch Med, TR-06560 Ankara, Turkey