The experience in treatment of dengue fever using antiviral drug riamilovir in the Republic of Guinea (case report)

Dengue fever is classified as one of the most common viral diseases with a transmission mechanism implemented through arthropod vectors. The expansion of of the Aedes aegypti mosquito is leading to a significant increase in the number of cases of dengue fever in more than 100 countries, highlighting the importance of developing and implementing specific prevention and treatment measures. Etiotropic drugs with proven efficacy against the pathogen are not registered, and the use of the vaccine is approved only among seropositive individuals. In this regard, pathogenetic treatment remains the main therapeutic strategy, however, work on the synthesis of antiviral drugs is being actively carried out. Due to the unique functions of non-structural proteins NS3 and NS5 in the viral replication cycle, they have become the main targets for studying the antiviral activity of a number of chemotherapy drugs. Of these proteins, due to the most conserved structure, the NS5 protein is a promising target for inhibition, however, success in obtaining a clinical effect using a number of available antiviral drugs has not been reached. This study describes the positive experience of using the nucleoside analogue riamilovir in the treatment of a patient with dengue fever in the Republic of Guinea

Functional Changes in the Snail Statocyst System Elicited by Microgravity

BACKGROUND: The mollusk statocyst is a mechanosensing organ detecting the animal's orientation with respect to gravity. This system has clear similarities to its vertebrate counterparts: a weight-lending mass, an epithelial layer containing small supporting cells and the large sensory hair cells, and an output eliciting compensatory body reflexes to perturbations. METHODOLOGY/PRINCIPAL FINDINGS: In terrestrial gastropod snail we studied the impact of 16- (Foton M-2) and 12-day (Foton M-3) exposure to microgravity in unmanned orbital missions on: (i) the whole animal behavior (Helix lucorum L.), (ii) the statoreceptor responses to tilt in an isolated neural preparation (Helix lucorum L.), and (iii) the differential expression of the Helix pedal peptide (HPep) and the tetrapeptide FMRFamide genes in neural structures (Helix aspersa L.). Experiments were performed 13-42 hours after return to Earth. Latency of body re-orientation to sudden 90° head-down pitch was significantly reduced in postflight snails indicating an enhanced negative gravitaxis response. Statoreceptor responses to tilt in postflight snails were independent of motion direction, in contrast to a directional preference observed in control animals. Positive relation between tilt velocity and firing rate was observed in both control and postflight snails, but the response magnitude was significantly larger in postflight snails indicating an enhanced sensitivity to acceleration. A significant increase in mRNA expression of the gene encoding HPep, a peptide linked to ciliary beating, in statoreceptors was observed in postflight snails; no differential expression of the gene encoding FMRFamide, a possible neurotransmission modulator, was observed. CONCLUSIONS/SIGNIFICANCE: Upregulation of statocyst function in snails following microgravity exposure parallels that observed in vertebrates suggesting fundamental principles underlie gravi-sensing and the organism's ability to adapt to gravity changes. This simple animal model offers the possibility to describe general subcellular mechanisms of nervous system's response to conditions on Earth and in space

Robotic automation of inland container terminals

The article presents the analysis of options for a transshipment terminal system with consideration of Russian transport system development. The aim is to determine the premises and possible problems, considering human absence, in the technological process at an inland container terminal. Statistical methods are used to analyze the market of robotic automation and the perspective for unmanned technology introduction. Simulation modeling of inland container terminal operation with various types of equipment, to study the applicability of robotic automation. The choice of modeling equipment results from the impossibility of completing an experiment on the real object, difficulties of analytical modeling (the system contains casual relations, nonlinear logic, stochastic variables), and the necessity to analyze the system’s time behavior. Consideration of robotic automation in a terminal warehouse complex is of particular importance due to technological progress followed by the freight terminal to be an area with highly organized technological processes and the need for highly paid specialists

Inversionless superradiance and the Duffing model

Superradiance of three-level optical systems with a doublet in the ground state (Lambda -scheme) placed in a high-Q cavity is studied theoretically. The conservation laws are obtained, which allow to considerably reduce the dimension of the phase space of the examined model (R^{11} -> R^{5}). In the particular case of a degenerate doublet, a mapping that makes it possible to reduce the problem of the three-level superradiance to a Duffing oscillator model (R^{5} -> R^{2}) is found. It is shown the possibility to initiate the superradiance generation even in the case when the population of the upper level is smaller than the total population of the lower doublet, i.e., without population inversion on the whole

New data on Upper Carboniferous - Lower Permian deposits of Bol’shevik Island, Severnaya Zemlya Archipelago

We present here a detailed study of the Upper Carboniferous–Lower Permian stratigraphy of Bol’shevik Island in the Severnaya Zemlya Archipelago, consisting of the analysis of sedimentary structures and lithostratigraphy, U/Pb detrital zircon dating and structural studies. The preserved sedimentary structures suggest that the studied strata were deposited in a relatively small meandering fluvial system. U/Pb dating of detrital zircons reveals that the Upper Carboniferous–Lower Permian sandstones contain a primary age population ranging from 450 to 570 millions of years, with a predominance of Early–Middle Ordovician zircons. This detrital zircon distribution indicates that the studied formations were derived locally from the erosion of Lower Ordovician deposits of Bol’shevik Island or elsewhere in the archipelago. Our structural studies suggest that Upper Carboniferous–Lower Permian deposits are deformed into a series of west–north-west verging open asymmetric folds, suggesting a west–north-west direction of tectonic transport and that deformation across the island is post-Early Permian in age.Keywords: Severnaya Zemlya Archipelago; Arctic; detrital zircon; Late Palaeozoic; tectonic.Citation: Polar Research 2015, 34, 24558, http://dx.doi.org/10.3402/polar.v34.24558(Published: 7 May 2015)To access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools)

Arctic Ocean Mega Project: Paper 3 - Mesozoic to Cenozoic geological evolution

We present an atlas of paleogeographic and paleotectonic maps which documents major events in the Arctic for 0–157 Ma. We demonstrate that the Mendeleev Ridge has a continental basement. The following chronology of events in the history of the Arctic Ocean is proposed: (1) Jurassic: continental rifting in the area of the Sverdrup-Banks basins and in the area of the present-day Canada Basin; a system of continental-margin volcanic belts formed in the region of Chukotka and the Verkhoyansk-Omolon; (2) Berriasian-Barremian: formation of the continental-margin Verkhoyansk-Chukotka Orogen; fast opening of Canada Basin (~133–125 Ma); (3) Aptian-Albian: formation of continental igneous provinces, rifting and magmatism in the area of the Alpha-Mendeleev ridges; rifting in the Ust’-Lena, Anisin, North-Chukchi, Podvodnikov and Toll basins; (4) Cenomanian-Campanian: intraplate magmatism in the area of the Alpha-Mendeleev ridges; (5) Campanian-Maastrichtian: a likely start of compressional deformations in the area of the Chukchi Sea; (6) Paleocene: formation of the continental-margin orogen; continental rifting along the present-day Eurasia Basin and the Ust’-Lena Basin; (7) Early-Middle Eocene: onset of opening of the Eurasia Basin started; (8) Middle-Late Eocene: a major restructuring of paleogeography of the Arctic took place at ca. 45 Ma with subaerial emergence of the Barents and Kara Sea shelves and onset of ultra-slow spreading of the Gakkel Ridge, and start of the epoch of formation of normal and strike-slip faults on the Lomonosov and Alpha-Mendeleev ridges and on the shelves of the Chukchi and East Siberian seas. Paleoclimate is discussed in connection with changes in the paleogeography

Arctic Ocean Mega Project: Paper 3 - Mesozoic to Cenozoic geological evolution

We present an atlas of paleogeographic and paleotectonic maps which documents major events in the Arctic for 0–157 Ma. We demonstrate that the Mendeleev Ridge has a continental basement. The following chronology of events in the history of the Arctic Ocean is proposed: (1) Jurassic: continental rifting in the area of the Sverdrup-Banks basins and in the area of the present-day Canada Basin; a system of continental-margin volcanic belts formed in the region of Chukotka and the Verkhoyansk-Omolon; (2) Berriasian-Barremian: formation of the continental-margin Verkhoyansk-Chukotka Orogen; fast opening of Canada Basin (~133–125 Ma); (3) Aptian-Albian: formation of continental igneous provinces, rifting and magmatism in the area of the Alpha-Mendeleev ridges; rifting in the Ust’-Lena, Anisin, North-Chukchi, Podvodnikov and Toll basins; (4) Cenomanian-Campanian: intraplate magmatism in the area of the Alpha-Mendeleev ridges; (5) Campanian-Maastrichtian: a likely start of compressional deformations in the area of the Chukchi Sea; (6) Paleocene: formation of the continental-margin orogen; continental rifting along the present-day Eurasia Basin and the Ust’-Lena Basin; (7) Early-Middle Eocene: onset of opening of the Eurasia Basin started; (8) Middle-Late Eocene: a major restructuring of paleogeography of the Arctic took place at ca. 45 Ma with subaerial emergence of the Barents and Kara Sea shelves and onset of ultra-slow spreading of the Gakkel Ridge, and start of the epoch of formation of normal and strike-slip faults on the Lomonosov and Alpha-Mendeleev ridges and on the shelves of the Chukchi and East Siberian seas. Paleoclimate is discussed in connection with changes in the paleogeography

Homologue of Protein Kinase Mζ Maintains Context Aversive Memory and Underlying Long-Term Facilitation in Terrestrial Snail Helix.

It has been shown that a variety of long-term memories in different regions of the brain and in different species are quickly erased by local inhibition of PKMζ. Using antibodies to mammalian PKMζ, we describe in the present study the localization of immunoreactive molecules in the nervous system of the terrestrial snail Helix lucorum. Presence of a homologue of PKMζ was confirmed with transcriptomics. We have demonstrated in behavioral experiments that contextual fear memory disappeared under a blockade of PKMζ with a selective peptide blocker of PKMζ (ZIP), but not with scrambled ZIP. If ZIP was combined with a reminder (20 min in noxious context), no impairment of the long-term contextual memory was observed. In electrophysiological experiments we investigated whether PKMζ takes part in the maintenance of long-term facilitation (LTF) in the neural circuit mediating tentacle withdrawal. LTF of excitatory synaptic inputs to premotor interneurons was induced by high-frequency nerve stimulation combined with serotonin bath applications and lasted at least four hours. We found that bath application of 2x10-6 M ZIP at the 90th min after the tetanization reduced the EPSP amplitude to the non-tetanized EPSP values. Applications of the scrambled ZIP peptide at a similar time and concentration didn't affect the EPSP amplitudes. In order to test whether effects of ZIP are specific to the synapses, we performed experiments with LTF of somatic membrane responses to local glutamate applications. It was shown earlier that serotonin application in such an artificial synapse condition elicits LTF of responses to glutamate. It was found that ZIP had no effect on LTF in these conditions, which may be explained by the very low concentration of PKMζ molecules in somata of these identified neurons, as evidenced by immunochemistry. Obtained results suggest that the Helix homologue of PKMζ might be involved in post-induction maintenance of long-term changes in the snail