Über Opilio canestrinii

Die Kurzmitteilung über Opilio canestrinii behandelt die Phänologie und weitere Aspekte dieser Spinnenart anhand von Untersuchungen bei Frankfurt am Main (Hessen)

Enoplognatha militaris is a synonym of Enoplognatha latimana (Araneae: Theridiidae)

Bei einer Untersuchung mit Bodenfallen im Rahmen der Biotopkartierung der Stadt Frankfurt am Main (BÖNSEL et al., im Druck fand sich auf dem Gelände des Hauptgüterbahnhofs (TK 5817) in dem Tiermaterial aus dem Zeitraum 21.7.-25.8.1998 ein Männchen einer Enoplognatha-Art, das aufgrund der Ausprägung des Konduktors scheinbar keiner der häufigeren Enoplognatha-Arten zuzuordnen war und auch mit Hilfe der gängigen Bestimmungsliteratur nicht bestimmt werden konnte

List of endangered spider species of Germany (Red Data Book) (Arachnida: Araneae)

Seit der Bearbeitung der 1. Fassung der Roten Liste der Spinnen Deutschlands durch HARMS (1984) sind mehr als 10 Jahre vergangen. Die Kenntnisse über Verbreitung, Ökologie und Gefährdung dieser Tiergruppe haben sich seither erheblich vermehrt, Überwiegend durch die Ergebnisse faunistisch-ökologischer Untersuchungen im Rahmen von Gutachten, z. B. für Umweltverträglichkeitsprüfungen oder Unterschutzstellungsverfahren

Sanskrit Lexical Sources: Digital Synthesis and Revision

The proposed project aims to synthesize, extend, revise, and improve the principal lexical reference works of Sanskrit, one of the world's richest culture-bearing languages, and to provide wide public access to them in the digital Sanskrit library

High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium

BACKGROUND: During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of β-lactam antibiotics were systematically improved. RESULTS: For this purpose, the PGA leader peptide was replaced by the B. megaterium LipA counterpart. A production strain deficient in the extracellular protease NprM and in xylose utilization to prevent gene inducer deprivation was constructed and employed. A buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production was developed in microscale cultivations and scaled up to a 2 Liter bioreactor. Productivities of up to 40 mg PGA per L growth medium were reached. CONCLUSION: The combination of genetic and medium optimization led to an overall 7-fold improvement of PGA production and export in B. megaterium. The exclusion of certain amino acids from the minimal medium led for the first time to higher volumetric PGA activities than obtained for complex medium cultivations

Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase

A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivation and induction conditions, proteomic analysis was carried out by combined use of two-dimensional gel electrophoresis and mass spectrometry (2-DE/MS) for protein separation and identification. 2-DE method was optimized for the separation of intracellular proteins. Since the genome of B. megaterium is not yet available, peptide sequencing using peptide fragment information obtained from nanoelectrospray ionization quadrupole-time-of-flight tandem mass spectrometry (ESI-QqTOF MS/MS) was applied for protein identification. 167 protein spots were identified as 149 individual proteins, including most enzymes involved in the central carbon metabolic pathways and many enzymes related to amino acid synthesis and protein synthesis. Based on the results a 2-DE reference map and a corresponding protein database were constructed for further proteomic approaches on B. megaterium. For the first time it became possible to perform comparative proteomic analysis on B. megaterium in a batch culture grown on glucose with xylose induction for dextrasucrase production. No significant differences were observed in the expression changes of enzymes of the glycolysis and TCA cycle, indicating that dextransucrase production, which amounted to only 2 % of the entire protein production, did not impose notable metabolic or energetic burdens on the central carbon metabolic pathway of the cells. However, a short-term up-regulation of aspartate aminotransferase, an enzyme closely related to dextransucrase production, in the induced culture demonstrated the feasibility to use 2-DE method for monitoring dextransucrase production. It was also observed that under the cultivation conditions used in this study B. megaterium tended to channel acetyl-CoA into pathways of polyhydroxybutyrate production. No expression increases were found with cytosolic chaperones such as GroEL and DnaK during dextransucrase production and secretion, whereas a strong up-regulation of the oligopeptide-binding protein OppA was observed in correlation with an increased secretion of dextransucrase into the culture medium

A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His(6)-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography

More efficient comparison protocols for MPC

In 1982, Yao introduced the problem of comparing two private values, thereby launching the study of protocols for secure multi-party computation (MPC). Since then, comparison protocols have undergone extensive study and found widespread applications. We survey state-of-the-art comparison protocols for an arbitrary number of parties, decompose them into smaller primitives and analyse their communication complexity under the usual assumption that the underlying MPC protocol does preprocessing and computes linear operations without communication. We then develop two new comparison protocols and explain why they are faster than similar protocols, including those that are commonly used in practice: they reduce the number of online multiplications, without increasing preprocessing or round complexity. More concretely, online bandwidth is reduced by more than half for the standard comparison protocols whose round complexity is logarithmic in the bit-length, whereas for constant round comparison protocols the reduction is two-thirds