5 research outputs found
Eine tierexperimentelle Studie
Get PDFThe most common cause of implant failure in joint replacement is aseptic loosening due to particle-induced osteolysis. We investigated the effect of a single subcutaneous dose of zoledronic acid (ZA) on particle-induced osteolysis.
We utilized the murine calvaria osteolysis model in C57BL/J6 mice. Bone resorption was measured as resorption within the midline suture using Giemsa staining. Osteoclast numbers were measured per high power field using TRAP-staining. The osteoid tissue area determined. Twenty-eight mice were used, seven per group. For statistical analysis one-way ANOVA and a Student’s t-test used.
Bone resorption was 0.26mm2 ± 0.09mm2 in animals with particle implantation, 0.14mm2 ± 0.05 mm2 in animals with particle implantation and ZA treatment directly after surgery (p=0.0047), and 0.15 mm2 ± 0.05 mm2 in animals with particle implantation and ZA treatment on the fourth postoperative day (p=0.006). The osteoclast number was 8.7 ± 2.8 in animals without particle implantation and 20 ± 4 in animals with particle implantation, compared to 9.2 ± 2.5 in animals with particle implantation and ZA treatment directly after surgery (p=0.0001), and 10.3 ± 1.4 in animals with particle implantation and ZA treatment on the fourth postoperative day (p=0.0004).
Net bone growth was significantly increased in animals with zoledronic acid treatment: 0.02 mm2 ± 0.03 mm2 in animals with particle implantation only (group 2), 0.25 mm2 ± 0.08 mm2 with particle implantation and zoledronic acid treatment directly after surgery (group 3; p=0.0018), and 0.21 mm2 ± 0.11 mm2 with particle implantation and zoledronic acid treatment on the fourth postoperative day (group 4; p=0.0042).
In summary, using the murine calvarial osteolysis model, particle-induced bone resorption was markedly decreased by a single s.c. dose of ZA and Zoledronic acid is a potent anti-resorptive drug and may also stimulate bone apposition locally in the process of particulate-induced osteolysis
Interactions between mammalian cells and nano- or micro-sized wear particles: Physico-chemical views against biological approaches
Get PDFTotal joint arthroplasty (TJA) is a more and more frequent approach for the treatment of end-stage osteoarthritis in young and active adults; it successfully relieves joint pain and improves function significantly enhancing the health-related quality of life. Aseptic loosening and other wear-related complications are some of the most recurrent reasons for revision of TJA. This review focuses on current understanding of the biological reactions to prosthetic wear debris comparing in vivo and in vitro results. Mechanisms of interactions of various types of cells with metal, polymeric and ceramic wear particles are summarised. Alternative views based on multidisciplinary approaches are proposed to consider physico-chemical, surface parameters of wear particles (such as: particle size, geometry and charge) and material (particle chemical composition and its nature) with biological effects (cellular responses)
