1 research outputs found
Pyrene Excimer-Based Peptidyl Chemosensors for the Sensitive Detection of Low Levels of Heparin in 100% Aqueous Solutions and Serum Samples
Fluorescent chemosensors (<b>1</b> and <b>2</b>, Py–(Arg)<sub><i>n</i></sub>GlyGlyGlyÂ(Arg)<sub><i>n</i></sub>LysÂ(Py)–NH<sub>2</sub>, <i>n</i> = 2 and 3) bearing
two pyrene (Py) labeled heparin-binding peptides were synthesized
for the sensitive ratiometric detection of heparin. The peptidyl chemosensors
(<b>1</b> and <b>2)</b> sensitively detected nanomolar
concentrations of heparin in aqueous solutions and in serum samples
via a ratiometric response. In 100% aqueous solutions at pH 7.4, both
chemosensors exhibited significant excimer emission at 486 nm as well
as weak monomer emission in the absence of heparin. Upon the addition
of heparin into the solution, excimer emission increased with a blue
shift (10 nm) and monomer emission at 376 nm decreased. The chemosensors
showed a similar sensitive ratiometric response to heparin independent
of the concentration of the chemosensors. The peptidyl chemosensors
were applied to the ratiometric detection of heparin over a wide range
of pH (1.5–11.5) using the excimer/momomer emission changes.
In the presence of serum, <b>1</b> and <b>2</b> displayed
significant monomer emission at 376 nm with relatively weak excimer
emission and the addition of heparin induced a significant increase
in excimer emission at 480 nm and a concomitant decrease in monomer
emission. The enhanced ratiometric response to heparin in the serum
sample was due to the interactions between the peptidyl chemosensors
and serum albumin in the serum sample. The detection limits of <b>2</b> for heparin were less than 1 nM in 100% aqueous solutions
and serum samples. The peptidyl chemosensors bearing two heparin-binding
sites are a suitable tool for the sensitive ratiometric detection
of nanomolar concentrations of heparin in 100% aqueous solutions and
serum samples