Polarographic Studies on La(III), Sm(III), Eu(III) & Yb(III) Complexes with Diethylenetriaminepentaacetic, Triethylenetetraminehexaacetic & Tetraethylenepentamineheptaacetic Acids

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Effect of gamma ray irradiation doses on pollen viability and in-vitro germination in Citrus

Induction of haploid plants in fruit crops through gamma irradiated pollen technique is of paramount importance in modern fruit breeding to reduce the breeding cycle. But the major problem of this technique is the sensitivity of pollen grains to higher irradiation doses. Present work analyzed the viability, in vitro germinability and pollen tube growth of gamma ray irradiated pollens of 2 different Citrus species: C. limetta, and C. sinensis. Both viability and in vitro germination capacity of irradiated pollen decreased gradually with increasing concentrations of irradiation in both the pollen parent; however, at highest irradiation dose (400 Gy), reduction of pollen viability and in vitro germination capacity, as compared to respective controls was recorded minimum in C. limetta (11.07 and 29.78%, respectively). In vitro pollen tube length as measured at 24, 48 and 72 hr after incubation, was found maximum in C. limetta (267.83, 303.24 and 325.57 μm, respectively). Our experiment revealed that pollen of C. limetta has higher resistance to gamma ray irradiation as compared to C. sinensis, hence C. limetta can be employed as more reliable pollen parent in haploidy programme of Citrus

Electrochemical sensing of hydrogen peroxide based on nano γ-Fe2O3 modified glassy carbon electrode

162-166Maghemite (γ-Fe2O3) nanoparticles were prepared using chemical synthesis method and used for sensing the hydrogen peroxide. The morphology of the γ-Fe2O3 nanoparticles was characterized by scanning electron microscopy. The γ-Fe2O3 nanoparticles were used to modify glassy carbon electrode (GCE) to form nano γ-Fe2O3 modified GC electrode for electrochemical sensing of hydrogen peroxide (H2O2). A potential controlled cyclic voltammetric (CV) technique was performed to sense hydrogen peroxide using nano γ-Fe2O3 modified GC electrode. The nano γ-Fe2O3 modified GC electrode showed excellent electrocatalytic ability towards H2O2 in 0.1M KCl supporting electrolyte. A significant enhancement in anodic peak current was observed for the nano γ-Fe2O3 modified GC electrode than those of bare electrode. The electrochemical characteristics of hydrogen peroxide on nano γ-Fe2O3 modified GC electrode  had been explored in this research communication

Electrochemical sensing of hydrogen peroxide based on nano γ-Fe2O3 modified glassy carbon electrode

Maghemite (γ-Fe2O3) nanoparticles were prepared using chemical synthesis method and used for sensing the hydrogen peroxide. The morphology of the γ-Fe2O3 nanoparticles was characterized by scanning electron microscopy. The γ-Fe2O3 nanoparticles were used to modify glassy carbon electrode (GCE) to form nano γ-Fe2O3 modified GC electrode for electrochemical sensing of hydrogen peroxide (H2O2). A potential controlled cyclic voltammetric (CV) technique was performed to sense hydrogen peroxide using nano γ-Fe2O3 modified GC electrode. The nano γ-Fe2O3 modified GC electrode showed excellent electrocatalytic ability towards H2O2 in 0.1M KCl supporting electrolyte. A significant enhancement in anodic peak current was observed for the nano γ-Fe2O3 modified GC electrode than those of bare electrode. The electrochemical characteristics of hydrogen peroxide on nano γ-Fe2O3 modified GC electrode  had been explored in this research communication

Effect of farmyard manure, organic manure and balanced fertilizers application on the productivity and soil fertility in pearl millet (Pennisetum glaucum)- mustard (Brassica juncea) cropping sequence in sandy loam soil of semi-arid regions

The experiment was conducted at the research farm of Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar from 2007-08 to 2009-10 to study the effect of farmyard manure and balanced fertilizers on production potential, economic viability and soil properties in the pearl millet [Pennisetum glaucum (L) R. Br.]- mustard [Brassica juncea (L.) Czernj. and Coss.] cropping sequence. The study consisted of 12 treatment combinations of farmyard manure and inorganic fertilizers with micronutrients were laid out in Factorial Randomized Block Design with three replications. The mean data revealed that use of balanced fertilizers (Potash, gypsum, ZnSO4 and FeSO4) along with application of 5.0 tonnes FYM/ha in pearl millet-mustard crop sequence produced 7.5 per cent higher pearl millet grain yield (3.59 tonnes/ha) and 8.2 per cent more mustard seed yield (1.96 tonnes/ha) than no FYM application (3.34 tonnes/ha by pearl millet and 1.81 tonnes/ha by mustard). Among different balanced nutrient treatments; recommended dose of nitrogen and phosphorous for both the crops, i.e. 120 kg N + 60 kg P/ha for pearl millet and 80 kg N + 30 kg P/ha for mustard along with 5.0 tonnes FYM/ha + 20 kg K2O/ha + 200 kg gypsum +10 kg ZnSO4 /ha + 10 kg FeSO4/ha (F5) produced maximum pearl millet grain yield (3.91 tonnes/ha) and mustard seed yield (2.31 t/ha), pearl millet equivalent yield (11.91 t/ha) and gross returns (Rupees93 051/ha) whereas, maximum net returns (RUPEES 44 529 /ha) and B:C ratio (1:93) were observed in the treatment 5.0 t FYM /ha + RD of N and P +20 kg K2O/ha + 200 kg gypsum/ha + 10 kg ZnS04 kg/ha (F4). The F5 treatment decreased pH (7.8) and EC (0.33 ds/m), improved the organic carbon (0.37%), available N (205 kg/ha), P2O5 (17.7 kg/ha), K2O (331 kg/ha), Fe (3.48 ppm), Zn (2.30 ppm) and S (4.30 ppm) status in comparison to values of 8.0 and 0.36. ds/m 0.34%, 196 kg/ha, 17.3 kg/ha, 323.7 kg/ha, 3.36 ppm, 2.15 ppm and 4.45 ppm, respectively in RD of N and P + 20 kg K2O/ha + 200 kg gypsum/ha + 10 kg ZnSO4/ha + 10 kg FeSO4/ha treatment when no FYM was used. The quality traits; protein content in pearl millet, oil content and oil yield were also found superior in the F5 treament than all other combinations of balanced fertilizers with and without 5.0 tonnes FYM/ha

Global, regional, and national burden of osteoarthritis, 1990–2020 and projections to 2050: a systematic analysis for the Global Burden of Disease Study 2021

Background Osteoarthritis is the most common form of arthritis in adults, characterised by chronic pain and loss of mobility. Osteoarthritis most frequently occurs after age 40 years and prevalence increases steeply with age. WHO has designated 2021–30 the decade of healthy ageing, which highlights the need to address diseases such as osteoarthritis, which strongly affect functional ability and quality of life. Osteoarthritis can coexist with, and negatively effect, other chronic conditions. Here we estimate the burden of hand, hip, knee, and other sites of osteoarthritis across geographies, age, sex, and time, with forecasts of prevalence to 2050. Methods In this systematic analysis for the Global Burden of Disease Study, osteoarthritis prevalence in 204 countries and territories from 1990 to 2020 was estimated using data from population-based surveys from 26 countries for knee osteoarthritis, 23 countries for hip osteoarthritis, 42 countries for hand osteoarthritis, and US insurance claims for all of the osteoarthritis sites, including the other types of osteoarthritis category. The reference case definition was symptomatic, radiographically confirmed osteoarthritis. Studies using alternative definitions from the reference case definition (for example self-reported osteoarthritis) were adjusted to reference using regression models. Osteoarthritis severity distribution was obtained from a pooled meta-analysis of sources using the Western Ontario and McMaster Universities Arthritis Index. Final prevalence estimates were multiplied by disability weights to calculate years lived with disability (YLDs). Prevalence was forecast to 2050 using a mixed-effects model. Findings Globally, 595 million (95% uncertainty interval 535–656) people had osteoarthritis in 2020, equal to 7·6% (95% UI 6·8–8·4) of the global population, and an increase of 132·2% (130·3–134·1) in total cases since 1990. Compared with 2020, cases of osteoarthritis are projected to increase 74·9% (59·4–89·9) for knee, 48·6% (35·9–67·1) for hand, 78·6% (57·7–105·3) for hip, and 95·1% (68·1–135·0) for other types of osteoarthritis by 2050. The global age-standardised rate of YLDs for total osteoarthritis was 255·0 YLDs (119·7–557·2) per 100 000 in 2020, a 9·5% (8·6–10·1) increase from 1990 (233·0 YLDs per 100 000, 109·3–510·8). For adults aged 70 years and older, osteoarthritis was the seventh ranked cause of YLDs. Age-standardised prevalence in 2020 was more than 5·5% in all world regions, ranging from 5677·4 (5029·8–6318·1) per 100 000 in southeast Asia to 8632·7 (7852·0–9469·1) per 100 000 in high-income Asia Pacific. Knee was the most common site for osteoarthritis. High BMI contributed to 20·4% (95% UI –1·7 to 36·6) of osteoarthritis. Potentially modifiable risk factors for osteoarthritis such as recreational injury prevention and occupational hazards have not yet been explored in GBD modelling. Interpretation Age-standardised YLDs attributable to osteoarthritis are continuing to rise and will lead to substantial increases in case numbers because of population growth and ageing, and because there is no effective cure for osteoarthritis. The demand on health systems for care of patients with osteoarthritis, including joint replacements, which are highly effective for late stage osteoarthritis in hips and knees, will rise in all regions, but might be out of reach and lead to further health inequity for individuals and countries unable to afford them. Much more can and should be done to prevent people getting to that late stage

Reporting trends, practices, and resource utilization in neuroendocrine tumors of the prostate gland: a survey among thirty-nine genitourinary pathologists

Background: Neuroendocrine differentiation in the prostate gland ranges from clinically insignificant neuroendocrine differentiation detected with markers in an otherwise conventional prostatic adenocarcinoma to a lethal high-grade small/large cell neuroendocrine carcinoma. The concept of neuroendocrine differentiation in prostatic adenocarcinoma has gained considerable importance due to its prognostic and therapeutic ramifications and pathologists play a pivotal role in its recognition. However, its awareness, reporting, and resource utilization practice patterns among pathologists are largely unknown. Methods: Representative examples of different spectrums of neuroendocrine differentiation along with a detailed questionnaire were shared among 39 urologic pathologists using the survey monkey software. Participants were specifically questioned about the use and awareness of the 2016 WHO classification of neuroendocrine tumors of the prostate, understanding of the clinical significance of each entity, and use of different immunohistochemical (IHC) markers. De-identified respondent data were analyzed. Results: A vast majority (90%) of the participants utilize IHC markers to confirm the diagnosis of small cell neuroendocrine carcinoma. A majority (87%) of the respondents were in agreement regarding the utilization of type of IHC markers for small cell neuroendocrine carcinoma for which 85% of the pathologists agreed that determination of the site of origin of a high-grade neuroendocrine carcinoma is not critical, as these are treated similarly. In the setting of mixed carcinomas, 62% of respondents indicated that they provide quantification and grading of the acinar component. There were varied responses regarding the prognostic implication of focal neuroendocrine cells in an otherwise conventional acinar adenocarcinoma and for Paneth cell-like differentiation. The classification of large cell neuroendocrine carcinoma was highly varied, with only 38% agreement in the illustrated case. Finally, despite the recommendation not to perform neuroendocrine markers in the absence of morphologic evidence of neuroendocrine differentiation, 62% would routinely utilize IHC in the work-up of a Gleason score 5 + 5 = 10 acinar adenocarcinoma and its differentiation from high-grade neuroendocrine carcinoma. Conclusion: There is a disparity in the practice utilization patterns among the urologic pathologists with regard to diagnosing high-grade neuroendocrine carcinoma and in understanding the clinical significance of focal neuroendocrine cells in an otherwise conventional acinar adenocarcinoma and Paneth cell-like neuroendocrine differentiation. There seems to have a trend towards overutilization of IHC to determine neuroendocrine differentiation in the absence of neuroendocrine features on morphology. The survey results suggest a need for further refinement and development of standardized guidelines for the classification and reporting of neuroendocrine differentiation in the prostate gland