Effect of soil and water environment on typeability of PowerPlex Y (Promega) in selected tissue samples.

In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of death determined within the limit of 14 hours. Heart muscle, liver and lung specimens were stored in pond water, sea water, sand and peat soil. DNA was extracted by organic method from tissue samples collected in 7-day intervals. Liver specimens were typeable in all PowerPlex Y loci within 100 days of storage in pond water with gradual decline at DYS392 in sea water. Heart muscle specimens stored in pond water exhibited allelic loss at DYS19, DYS385, DYS389II and DYS392, while all loci were typeable in sea water stored samples. For lung specimens allelic loss was noted throughout the profile. Storage of liver specimens in peat soil for more than 14 days resulted in allelic drop-out, and after 21 days no profiles were typeable. Heart muscle specimens were typeable in all PowerPlex Y systems after 35-day storage in sand, while allelic drop-out and subsequent lack of profiles were noted after 14 and 35 days respectively. Lung specimens stored in garden soil exhibited allelic drop-out and subsequent lack of profiles after 7 and 21 days, respectively. All PowerPlex Y loci were typeable in the latter material in sand up to day 35 with gradual decline of longer amplicons (DYS19, DYS385, DYS389II and DYS392)

The evaluation of human papillomavirus and p53 gene mutation in benign and malignant conjunctiva and eyelid lesions.

Papillomas and squamous cell carcinomas are the most common conjunctival and eyelid lesions. The etiology isstill unclear and recently human papillomavirus infection and p53 gene mutation have been taken into consideration. Theaim of our study was the evaluation of HPV DNApresence and p53 gene mutation in 45 benign and 38 malignant squamouslesions of the conjunctiva and eyelid. For HPV detection PCR-RFLP and immunohistochemical reaction were used; for p53gene mutation PCR-SSCP was used. Only 8.8% papillomas, 9.1% squamous cell cancers and 3.7% basal cell cancers (usingPCR-RFLP method) and 26.6% papillomas, 7.4% squamous cell cancers and 9.1% basal cell cancers (using immunohistochemicalreaction) were HPV positive. p53 gene mutation was evaluated in 24.4% papillomas, 54.5% squamous cell cancersand 22.2% basal cell cancers; most commonly in 6 and 7 exon. Human papillomavirus infection, opposite to p53 genemutation, is not a significant etiological factor of the benign and malignant conjunctival and eyelid lesions development

Population Genetic Data of 30 Insertion-Deletion Markers in the Polish Population

(1) Background: Insertion-deletion (InDel) markers show the advantages of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) and are considered alternative markers in forensic genetics. (2) Methods: Allelic frequencies and corresponding forensic efficiency parameters of 30 autosomal polymorphic InDel loci included in the Investigator DIPplex kit (Qiagen) were obtained in a sample of 631 unrelated Polish individuals. Allelic frequency data were compared with those reported for selected populations (3) Results: All the loci conformed with Hardy-Weinberg equilibrium after applying a Bonferroni correction and no pair-wise significant linkage disequilibrium was detected. (4) Conclusions: DIPplex Kit differences were high among populations worldwide. The InDel markers are highly discriminating for human identification purposes in the Polish population

The expression data from microarrays experiments for <i>NLRP2</i> gene on admission (A) (p = 0.00001) and discharge (B) (p = 0.0003).

<p>The points present the expression level from all analyzed patients with CC genotype (red) and CG/GG (blue).</p

A univariate analysis for 5-year mortality in the derivation cohort.

<p>For all parameters only admission values were used.</p><p>A univariate analysis for 5-year mortality in the derivation cohort.</p

A univariate analysis for 3-year mortality in the validation cohort.

<p>For all parameters only admission values were used.</p><p>A univariate analysis for 3-year mortality in the validation cohort.</p

Baseline characteristics of the study and validation groups.

<p>Mean values with standard deviations are given, unless otherwise specified.</p><p>* CC homozygotes vs. G-allele carriers.</p><p>^{^} derivation vs. validation group.</p><p>^#Smoking status in the derivation group was available in 453 patients (in 213 CC homozygotes and 240 G-allele carriers). It was available in all patients from the validation group.</p><p>NA- data not available.</p><p>Baseline characteristics of the study and validation groups.</p

Principal component analysis of PBMCs gene expression profiles.

<p>PCA plot shows the first three principal components of microarray data in respect to their correlation.</p

Kaplan-Meier survival curves for CC homozygotes and G-allele carriers of the rs12526453 SNP in the derivation group (panel A) and in the validation group (panel B).

<p>The differences between the groups were statistically significant (p = 0.017 in the derivation group and p = 0.031 in the validation group, Cox`s F test).</p