Differences in the composition of the bacterial element of the urinary tract microbiome in patients undergoing dialysis and patients after kidney transplantation

IntroductionThe development of molecular biology methods and their application in microbial research allowed the detection of many new pathogens that cause urinary tract infections (UTIs). Despite the advances of using new research techniques, the etiopathogenesis of UTIs, especially in patients undergoing dialysis and patients after kidney transplantation, is still not fully understood.MethodsThis study aimed to characterize and compare the composition of the bacterial element of the urinary tract microbiome between the groups of patients undergoing dialysis (n = 50) and patients after kidney transplantation (n = 50), with positive or negative urine culture, compared to healthy individuals (n = 50).ResultsAsymptomatic bacteriuria was observed in 30% of the urine cultures of patients undergoing dialysis and patients after kidney transplantation, with Escherichia coli as the most dominant microorganism (73%) detected with the use of classical microbiology techniques. However, differences in the bacterial composition of the urine samples between the evaluated patient groups were demonstrated using the amplicon sequencing. Finegoldia, Leptotrichia, and Corynebacterium were found to be discriminative bacteria genera in patients after dialysis and kidney transplantation compared to the control group. In addition, in all of urine samples, including those without bacteriuria in classical urine culture, many types of bacteria have been identified using 16S rRNA sequencing.DiscussionThe revealed microbial characteristics may form the basis in searching for new diagnostic markers in treatment of patients undergoing dialysis and patients after kidney transplantation

THE CONTENT OF IRON AND MANGANESE AND SOME HEAVY METALS IN WELLS ON THE EXAMPLE OF WATER FROM THE WELLS OF GORZYCE COMMUNITY

The subject of the study was to estimate the quality of shallow groundwater from wells which are the source of drinking water. The study included 8 wells located in the municipality of Gorzyce. The results are presented as medium concentrations of the analyzed components during one year of observation. The results confirm that poorly protected wells are characterized by poorer quality water. In most of the analyzed cases, the average concentration of pollution indexes (this applies particularly to iron and manganese concentration) exceed the limits established for drinking water

THE CHEMISTRY OF DEFAULTING SHALLOW GROUNDWATER ON THE BASE ON SELECTED VILLAGEOF GORZYCE COMMUNE

In Poland about 40% of the population lives in a rural areas and despite of the great technical progress in the infrastructure, there are still areas without water supply system where portable water is drawn from the well. The subject of this work was to evaluate the quality of shallow well water (98–604 cm), which is a source of water for consumption. Studies covered 8 wells in the Gorzyce commune. The following chemical components: pH, dissolved oxygen, electrolytic conductivity, BOD5, nitrogen, total phosphorus and phosphates, and ions of SO4-, Na, K+, Ca++ and Mg++ were analyzed. The results of the studies are described by the mean concentrations of components in analyzed period of time. The results confirm the fact that, poorly protected wells have a worse quality of water. Moreover a few cases, the average concentration of the analyzed indexes exceed acceptable standards for drinking water

Recent Advances in Treatment Options for Childhood Acute Lymphoblastic Leukemia

Acute lymphoblastic leukemia is the most common blood cancer in pediatric patients. There has been enormous progress in ALL treatment in recent years, which is reflected by the increase in the 5-year OS from 57% in the 1970s to up to 96% in the most recent studies. ALL treatment is based primarily on conventional methods, which include chemotherapy and radiotherapy. Their main weakness is severe toxicity, which prompts dose reduction, decreases the effectiveness of the treatment, and, in some cases, can lead to death. Currently, numerous modifications in treatment regimens are applied in order to limit toxicities emerging from conventional approaches and improve outcomes. Hematological treatment of pediatric patients is reaching for more novel treatment options, such as targeted treatment, CAR-T-cells therapy, and immunotherapy. These methods are currently used in conjunction with chemotherapy. Nevertheless, the swift progress in their development and increasing efficacity can lead to applying those novel therapies as standalone therapeutic options for pediatric ALL