Allele frequencies (resistance-associated alleles) of <i>Cooperia oncophora</i> β-tubulin isotype 1 at codons 167, 198 and 200.

a<p>Comparison of different isolates/populations with the susceptible reference isolate <i>C.o.</i>sus. using ANOVA and Dunnett's post hoc test.</p>b<p>Not significant.</p

Phylogram showing relationship between nematode and <i>D. melanogaster</i> tubulins.

<p>Deduced proteins were aligned and the corresponding alignment of cDNAs with aligned codons was analyzed by maximum likelihood analysis as described in material and methods. Statistical support according to the Shimodaira-Hasegawa modification of the approximate likelihood test and the Bayesian transformation of the approximate likelihood ratio are reported before and after the slash. If only one number is given the results of both tests were identical. Accession numbers for all cDNAs are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070212#pone.0070212.s003" target="_blank">Table S2</a>. Species abbreviations: <i>Aca</i>, <i>Ancylostoma caninum</i>; <i>Alu</i>, <i>Ascaris lumbricoides</i>; <i>Asu</i>, <i>Ascaris suum</i>; <i>Bma</i>, <i>Brugia malayi</i>; <i>Can</i>, <i>Cylicocylus nassatus</i>; <i>Cbr</i>, <i>Caenorhabditis briggsae</i>; <i>Cca</i>, <i>Cylicocyclus catenatus</i>; <i>Cel</i>, <i>Caenorhabditis elegans</i>; <i>Con</i>, <i>Cooperia oncophora</i>; <i>Cpe</i>, <i>Cooperia pectinata</i>; <i>Dme</i>, <i>Drosophila melanogaster</i>; <i>Hco</i>, <i>Haemonchus contortus</i>; <i>Oos</i>, <i>Ostertagia ostertagia</i>; <i>Peq</i>, <i>Parascaris equorum</i>; <i>Tsp</i>, <i>Trichinella spiralis</i>; <i>Ttr</i>, <i>Trichuris trichiura</i>. An enlarged version of the β-tubulin subtree is provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070212#pone.0070212.s001" target="_blank">Figure S1</a>.</p

Regression analysis for pyrosequencing assays of <i>O. ostertagi</i> β-tubulin isotype 1.

<p>Artificial mixtures of respective PCR-products as templates containing varying amounts of copies with TAC in codon 168 (A), codon 198 (B) or codon 200 (C) were analyzed by pyrosequencing. Observed frequencies (mean ± SD) were plotted against input TAC frequencies in (A) and (C) and input GCA frequencies in (B). Regression plots (including linear equation and Pearson correlation coefficient) with 95% confidence bands are shown.</p

Allele frequencies (resistance-associated alleles) of <i>Ostertagia ostertagi</i> β-tubulin isotype 1 at codons 167, 198 and 200.

a<p>Comparison of different isolates/populations with the susceptible reference isolate <i>O.o.</i>sus. using ANOVA and Dunnett's post hoc test.</p>b<p>Not significant.</p