Evaluation of Forensic Genetic Parameters of 24 STR Loci and Y indel in a Southern Region Saudi Population Sample Using GlobalFiler™ PCR Amplification Kit

The last three decades have seen rapid advances in the field of short tandem repeats (STRs) genotyping technology. Autosomal STRs have emerged as a  powerful tool in forensic identification and paternity investigations. The indigenous population of Saudi Arabia is irregularly distributed and has historically been organized into geographically distinct groups or tribes of patrilineal descent. So far, there has been no detailed investigation of the southern region Saudi population to assist in the interpretation of DNA-based forensic evidence and in the construction of DNA database. The objective of this study is to investigate the genetic structure in 154 unrelated healthy Saudi subjects within three generations from the southern Saudi regions using a GlobalFiler™ PCR Amplification kit. Intra- and Inter-population genetic diversity as well as the forensic genetics parameters were analyzed. Our results showed that SE33 and TPOX loci were the most and the least polymorphic loci, respectively. The PIC, PE, TPI, Ho and He varied from 0.56116 (TPOX) to 0.94393 (SE33), 0.26638 (TPOX) to 0.83859 (SE33), 1.1875 (TPOX) to 6.33333 (SE33), 0.57894 (TPOX) to 0.92105 (SE33) and 0.6169 (TPOX) to 0.952 (SE33), respectively. The highest PM was observed for D22S1045 (0.223944) and the highest PD for SE33 (0.98935). The combined PD was 99.99999999% and the combined PM was equal to 3.19021E-25. Phylogenetic parameters showed that the southern region Saudi population had the closest genetic relationship with the Saudi, Emirati, Kuwaiti, and Bahraini populations. The study offers some important insights into the southern region Saudi population structure using GlobalFiler™ PCR Amplification kit

Burnout and Its Relation to Emotion Dysregulation and Social Cognition among Female Interns and Undergraduate Dental Students at King Khalid University

Background: Burnout is a state of vital exhaustion that has a high global prevalence among dental professionals. Yet the early diagnosis of this syndrome is challenging due to its multidimensional nature. Aim: To assess burnout and its relation to emotional dysregulation and social cognition among undergraduate dental students. Methods: We mailed 148 self-administered questionnaires to all interns and undergraduate dental students belonging to the College of Dentistry’s female campus at King Khalid University, Saudi Arabia. A questionnaire was designed comprising three measuring instruments of psychological inventories to assess work-related stress, namely, burnout (Copenhagen burnout inventory questionnaire (CBI)), emotional dysregulation (emotion regulation questionnaire (ERQ)), and social cognition (interpersonal reactivity index (IRI)). We examined the difference in burnout between two cohorts (interns and undergraduates) using Student’s ‘t’ test and the association between emotional regulation/social cognition domains using Pearson’s correlation. Results: Among the participants, around 70% obtained higher scores than the cut-off points for personal and work-related domains in both groups. There was no statistically significant difference in burnout domain between the two cohorts (p > 0.05; Cohen d < 0.3). A negative correlation existed between burnout and emotion regulation, i.e., higher burnout score is associated with lesser cognitive reappraisal and more expressive suppression (CBI—personal burnout r = −0.251; r = −0.220 respectively). Social cognitive scores had various associations among interns and undergraduates, with personal distress being significantly associated with greater burnout levels among both groups (p < 0.05). Conclusions: The present study revealed significantly elevated burnout levels among both interns and undergraduate students. Emotional dysfunction and social cognition assessment can be a valuable adjunct in identifying this erosive syndrome. Early recognition and primordial prevention targeting burnout are strongly recommended

Burnout and Its Relation to Emotion Dysregulation and Social Cognition among Female Interns and Undergraduate Dental Students at King Khalid University

Background: Burnout is a state of vital exhaustion that has a high global prevalence among dental professionals. Yet the early diagnosis of this syndrome is challenging due to its multidimensional nature. Aim: To assess burnout and its relation to emotional dysregulation and social cognition among undergraduate dental students. Methods: We mailed 148 self-administered questionnaires to all interns and undergraduate dental students belonging to the College of Dentistry’s female campus at King Khalid University, Saudi Arabia. A questionnaire was designed comprising three measuring instruments of psychological inventories to assess work-related stress, namely, burnout (Copenhagen burnout inventory questionnaire (CBI)), emotional dysregulation (emotion regulation questionnaire (ERQ)), and social cognition (interpersonal reactivity index (IRI)). We examined the difference in burnout between two cohorts (interns and undergraduates) using Student’s ‘t’ test and the association between emotional regulation/social cognition domains using Pearson’s correlation. Results: Among the participants, around 70% obtained higher scores than the cut-off points for personal and work-related domains in both groups. There was no statistically significant difference in burnout domain between the two cohorts (p > 0.05; Cohen d p Conclusions: The present study revealed significantly elevated burnout levels among both interns and undergraduate students. Emotional dysfunction and social cognition assessment can be a valuable adjunct in identifying this erosive syndrome. Early recognition and primordial prevention targeting burnout are strongly recommended

Prevalence, Predictors, and Awareness of Coffee Consumption and Its Trend among Saudi Female Students

This study aimed to investigate the prevalence, trends, and predictors of coffee consumption among Saudi female students and its association with anthropometric and demographic variables. A survey-based study using a face-to-face interview was designed, and 930 (aged 21.5 ± 2.1 years) apparently healthy female students from different departments of King Saud University participated. The prevalence of coffee consumption was significantly higher (88.2%, p < 0.03) in the central Riyadh region. Coffee consumers had significantly higher prevalence of being overweight than non-consumers (p = 0.02). The frequency of coffee consumption was significantly higher (p < 0.02) in students who were single and belonged to families with a moderate income level. Coffee consumption was significantly higher among first-year students with a high-scale grade point average (GPA) (p < 0.001 and p = 0.03, respectively). Increased coffee consumption during exam and stress conditions was associated with unhealthy dietary habits such as using more sugar and spices. The prevalence of coffee consumption was high among Saudi females. High body mass index (BMI) and increased family income level were strong determinants for coffee consumption. Continued nutritional education and awareness about the potential positive and negative health effects of coffee consumption and the importance of food label use should be provided to younger generations in order to correct the wrong perceptions

Biallelic variants in HECT E3 paralogs, HECTD4 and UBE3C, encoding ubiquitin ligases cause neurodevelopmental disorders that overlap with Angelman syndrome

Pathogenic variants in genes encoding ubiquitin E3 ligases are known to cause neurodevelopmental syndromes. Additional neurodevelopmental disorders associated with the other genes encoding E3 ligases are yet to be identified

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background: Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods: The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18-49, 50-69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results: NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion: As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population