EGCG, EGC and ECG suppressed FLT3 expression through Hsp90.

<p>MOLM-13 cells at a density of 1×10⁵ cells/ml were treated with 60 µM EGCG (A, lane 4), 100 µM EGC, 200 µM ECG (B, lane 2, 4) or DMSO alone as control for 8 hours. Total cell lysates were immunoprecipitated with anti-Hsp90. Precipitated protein were subjected to western blot analysis with anti-FLT3 and anti Hsp90. MOLM-13 cells treated with 2 µM 17-AAG was used as control (A, lane 2).</p

Down-regulation of FLT3 expression and its downstream molecules in EGC-treated AML cells.

<p>MOLM-13, MOLM-14, MV4-11 and KOCL-48 cells at a density of 1×10⁵ cells/ml were treated with indicated concentration of EGC or DMSO alone as control for 8 hours. Total cell lysates were subjected to western blot analysis with indicated antibodies.</p

Down-regulation of FLT3 expression and its downstream molecules in ECG-treated AML cells.

<p>MOLM-13, MOLM-14, MV4-11 and KOCL-48 cells at a density of 1×10⁵ cells/ml were treated with indicated concentration of ECG or DMSO alone as control for 8 hours. Total cell lysates were subjected to western blot analysis with indicated antibodies.</p

Isobolograms of simultaneous exposure to EGCG and PKC412 in MOLM-13, MOLM-14, MV4-11 and KOCL-48 cell lines.

<p>The isobolograms shown are representative of at least three independent experiments. Each point represents the mean value of at least three independent experiments. The combination of EGCG with PKC412 showed additive effect (MOLM-13 and MOLM-14) and antagonism effect (MV4-11 and KOCL-48).</p

Down-regulation of FLT3 expression and its downstream molecules in EGCG-treated AML cells.

<p>MOLM-13, MOLM-14, MV4-11, KOCL-48 and THP-1 cells at a density of 1×10⁵ cells/ml were treated with indicated concentration of EGCG or DMSO alone as control for 8 hours. Total cell lysates were subjected to western blot analysis with indicated antibodies.</p

EGCG, EGC and ECG induced apoptosis in MOLM-14 cells.

<p>MOLM-14 cells at a density of 1×10⁵ cells/ml were treated with 60 µM EGCG, 100 µM EGC, 200 µM ECG or DMSO alone as control for 16 hours. Total cell lysates were subjected to western blot analysis with indicated antibodies (A) or staining with PE-Annexin V and analyzed by FACS Calibur. Collected data were analyzed by FlowJo software (B).</p

Mean values of observed data and predicted minimum and maximum values of the combination of EGCG and PKC412.

<p>Mean values of observed data and predicted minimum and maximum values of the combination of EGCG and PKC412.</p