The effect of intimacy and communication media on self-disclosure

本研究は,自己開示に及ばす相手との親密度とコミュニケーションメディアの影響を検討することを目的とした.コミュニケーションメディアとしては,対面,携帯電話,携帯メールを設定し,相手との親密度は高,低の2条件を設定した.コミュニケーションメディアを被験者内要因,親密度を被験者間要因の混合計画とし,大学生(高親密度条件が39名,低親密度条件が」5名)を対象に実験を行った.その際,先行研究を基に,次の3つの仮説を立てた.まず仮説iとして,コミュニケーションメディアの種類を問わず,高槻密度条件のほうが低親密度条件に比べて,自己開示量が多くなると推測した.仮説2として,高親密度条件では,対面条件において自己開示が最も多くなると推測した.仮説3として,低親密度条件では,携帯メール条件において自己開示が最も多くなると推測した.実験の結果,仮説1,仮説2は支持された.しかし,低親密度条件では,高親密度条件と同様,対面条件で自己開示崖が最も多くなり,仮説3は支持されなかった

Additional file 3 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 3. Unprocessed western blot images for Fig. 1. Unprocessed western blot images for Fig. 2. Unprocessed western blot images for Fig. 3. Unprocessed western blot images for Figure S3. Unprocessed western blot images for Figure S4

Additional file 2 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 2: Table S3

Additional file 1 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 1: Figure S1. Relative CTGF and BAMBI mRNA levels in MM cells knocked down by transfection of CTGF-targeted siRNA. Relative mRNA expression levels of BAMBI in MSTO-211H, NCI-H28, Y-MESO-8D, NCI-H2052 and NCI-H2452 cells transfected with a CTGF-targeted siRNA (20nM) are shown. BAMBI mRNA expression level was downregulated by transfection of CTGF-targeted siRNA in MSTO-211H, NCI-H28, Y-MESO-8D and NCI-H2052 cells, but not in NCI-H2452 cells. Figure S2. Relative BAMBI mRNA levels and cell proliferation rates of cells knocked down by Santa Cruz's BAMBI siRNA pool. A, Relative mRNA expression levels of BAMBI in Y-MESO-14 and Y-MESO-27 cells transfected with a Santa Cruz's BAMBI siRNA pool (20nM). BAMBI mRNA expression level was downregulated by transfection of siRNA in Y-MESO-14 and Y-MESO-27 cells. B, Graphs of proliferation rates as measured by viable cell counting assay. Figure S3. Knockdown of BAMBI does not affect the TGF-β/Smad activation in Y-MESO-27 cells. Western blotting showed p-Smad2 and p-Smad3 levels in Y-MESO-27 cells. Cells were transfected with a BAMBI-targeted siRNA (20 nM) and compared with nontransfected cells and cells transfected with control siRNA. Cells were stimulated by adding exogenous TGF-β1 and compared with cells without exogenous TGF-β1. Figure S4. Expression and subcellular localization of BAMBI protein in mesothelial and MM cell lines. A, Western blots showing BAMBI levels in seven MM cell lines and the mesothelial MeT-5A cell line. BAMBI protein was ubiquitously expressed by all mesothelioma cell lines but only weakly or below detection limits by MeT-5A cells. B, Immunofluorescence staining of Y-MESO-27 and MeT-5A cells showing that BAMBI localizes to the plasma membrane and cytosol. The nuclei were counterstained with DAPI (blue). The white arrows demarcate BAMBI expression in the cell membrane. Scale bars: 10 μm. Table S1. the oligonucleotide sequences of the siRNAs used in the study. Table S2. the oligonucleotide sequences and accession number of primers used in the study