The recognition of HIG2 and HLA-A*02:01-expressing cells by a HIG2-9-4 peptide-specific CTL clone.

<p>(a) A HIG2-9-4 peptide-specific CTL clone was stimulated with COS7 cells expressing both HIG2 and HLA-A*02:01 (close diamond), or either HIG2 alone (open circle) or HLA-A*02:01 alone (open triangle), then the IFN-γ production was examined by ELISA. R/S ratio; responder/stimulator ratio. (b) The cytotoxic activity of the HIG2-9-4 peptide-specific CTL clone was examined against HLA-A*02:01-positive HIG2-expressing A498 cells (closed diamond) or HLA-A*02:01-negative HIG2-expressing Caki-1 cells (open circle). E/T ratio; effector/target ratio. All experiments were performed in triplicate. Representative results from three independent experiments are shown. *; <i>P</i><0.001.</p

The HLA-A*02:06-restricted response of a HIG2-9-4 peptide-specific CTL clone.

<p>(a) A HIG2-9-4 peptide-specific CTL clone was induced from HLA-A*02:06-positive PBMCs, and stimulated with HLA-A*02:06-positive PSCCA0922 cells pulsed with the HIG2-9-4 peptide (close diamond) or HIV peptide (open square). (b) The HIG2-9-4 peptide-specific CTL clone was stimulated with COS7 cells expressing both HIG2 and HLA-A*02:06 (close diamond), or either HIG2 alone (open circle) or HLA-A*02:06 alone (open triangle). The IFN-γ production in the culture supernatant was examined by ELISA. R/S ratio; responder/stimulator ratio. The representative results from three independent experiments are shown.</p

Candidate peptides derived from HIG2 restricted with HLA-A*02:01.

<p>The binding score was obtained from the BIMAS website (<a href="http://www-bimas.cit.nih.gov/molbio/hla_bind" target="_blank">http://www-bimas.cit.nih.gov/molbio/hla_bind</a>).</p

The expression of a HIG2-9-4 peptide-specific T cell receptor on CD8+ T cells.

<p>The expression of the HIG2-9-4 peptide-specific T cell receptor was examined on CD3⁺CD4⁻ cells following CTL expansion culture of HIG2-9-4 peptide-induced CTLs. (a) A quadrant gate was set based on the staining results with the HIV peptide/HLA-A*02: 01 tetramer. (b) CD8⁺ T cells expressing the HIG2-9-4 peptide/HLA-A*02: 01-specific T cell receptor were detected. Similar results were obtained from three independent experiments.</p