The permeability of PN mouse and pig embryos to COOH-PLL.

<p>The embryos were exposed to FITC-labeled COOH-PLL (5% (w/v)) for 5 min. After exposure, embryos with or without washing were examined under a laser scanning microscope. Scale bars denote 50 um.</p

The effect of COOH-PLL concentration on the number of cells in a blastocyst.

<p>The effect of COOH-PLL concentration on the number of cells in a blastocyst.</p

The effects of COOH-PLL on survival, fertility and developmental ability of vitrified mouse oocytes after IVF.

<p>Data are shown as means ± S.E.M. Different superscripts denote a significant difference (P<0.05). Numbers of oocytes used in each group were described under the treatment group.</p

The kinetics of COOH-PLL in mouse oocytes.

<p>Mouse oocytes were exposed to PB1(-) supplement with FITC-labeled COOH-PLL (5% (w/v)). Green: FITC-labeled COOH-PLL.</p

<i>In vivo</i> development of porcine embryos vitrified with P20.

<p><i>In vivo</i> development of porcine embryos vitrified with P20.</p

Cryoprotectants in equilibration and vitrification medium for mouse oocytes.

<p>*These cryoprotectants were added into calcium free PB1 supplemented with 20% FCS.</p><p>^# These cryoprotectants were added into calcium free PB1 supplemented with 20% FCS and 0.5 M sucrose.</p

The developmental ability of PN porcine embryos exposed to various concentrations of COOH-PLL.

<p>Data are mean ± SEM. Data was analyzed by Kruskal-Wallis test. There were no significant differences in the cleavage rate or blastocyst rate among the treatments (<i>p</i>>0.05). Number of oocytes used in each group were described under each treatment group.</p

In vivo development of mouse oocytes vitrified with COOH-PLL.

<p>In vivo development of mouse oocytes vitrified with COOH-PLL.</p

Piglets derived from PN porcine embryos vitrified with COOH-PLL.

<p>Piglets derived from PN porcine embryos vitrified with COOH-PLL.</p