2 research outputs found
DETERMINATION OF ALLICIN IN ALLIUM SATIVUM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND STUDY OF GENOTOXIC EFFECT ON HUMAN LEUKOCYTES
Objectives: Allicin is an organosulfur compound featuring thiosulfinate functional group. The compound is formed in garlic after tissue damage, bythe action of enzyme alliinase on alliin. This study involves extraction of allicin from garlic using high-performance liquid chromatography (HPLC) andstudy of it is the genotoxic effect on human leukocytes.Methods: A simple and rapid reverse phase HPLC was used for the extraction of allicin. Quality allicin, in conjugation with chymosin, was used tostudy its genotoxic effect on leukocytes.Results: Garlic oil and garlic extract showed similar retention time, and we measured the products using genotoxic effects in human leukocyte cultureand it shows statistically not significant.Conclusion: This study suggested that to take a lower concentration of garlic extracts benefits for health and these findings useful for further research.Keywords: Allium sativum, Leukocytes, Chromosome, Genotoxic, High-performance liquid chromatography
Carbon-Dot-Coated Alginate Beads as a Smart Stimuli-Responsive Drug Delivery System
In this work, we
report a smart stimuli-responsive drug delivery system (DDS) that
can release drug depending upon the amount of pathogen (MRSA) present
in the target. A greater amount of MRSA in the system will lead to
more release of drug and vice versa. Carbon-dot-coated novel alginate
beads (CA-CD) exhibiting superior stability was successfully used
as smart drug delivery vehicle. Garlic extract (GE), which contains
allicin, was taken as model drug system to demonstrate the phenomena.
It was observed that GE loading was 19 and 78% with CA and CA-CD,
respectively. CA-CD-GE shows pH-dependent controlled drug release,
which results in increased therapeutic efficiency. CA-CD-GE is not
only stimuli responsive but also a controlled drug release system
as it releases drug according to the pathogen concentration (MRSA).
All the three factors viz. drug release, MRSA concentration and pH
of the medium are interdependent as when the cell divides, it produces
secondary metabolites that lead to the decrease in pH of the medium.
The drop in the pH value triggers drug release from the beads. And
the effect of the drug is reflected by the MRSA cell death. Hence,
we demonstrate a smart stimuli responsive DDS. However, such DDS will
be useful in cases where increased amount of pathogen in the system
will lead to reduction in pH