Synthesize of pluronic-based nanovesicular formulation loaded with Pistacia atlantica extract for improved antimicrobial efficiency

One of the current concerns to human health is antibiotic resistance, which promotes the use of antibiotics that are more harmful, expensive, and ineffective. In this condition, researchers are turning to innovative options to combat this alarming situation. Combining herbal medicine with nanotechnology has created a new strategy to increase the effectiveness of phytochemical compounds in overcoming antimicrobial resistance. Pistacia atlantica is one of the promising herbs with medicinal benefits, but its poor solubility in biological fluids is challenging. In this regard, we seek to evaluate the antibacterial efficacy of Pistacia atlantica extract-loaded nanovesicle. Cholesterol, Span 40, and Pluronic F127 modified nanoformulation was developed using an environmentally friendly improved heating technique, and it was evaluated for size distribution, zeta potential, morphology, entrapment efficiency (EE%), release behavior, stability, and antimicrobial performance. By using DLS, spherical nanovesicles were identified with a size distribution of 50–150 nm and a zeta potential of −43 mV. The extract's encapsulation efficiency was 72.03%. The developed loaded nanovesicles demonstrated controlled extract release in the tested 96 h and storage stability of at least 12 months at 25 °C. Also, Comparing the two samples, the encapsulated extract had greater antibacterial activity against Candida albicans, Staphylococcus aureus, and Pseudomonas plecoglossicida with MIC of 1320, 570, and 1100 µg/mL, respectively. Besides reducing the misuse of antibiotics by allowing for the controlled release of drugs made from natural sources, we expect the findings described here to help provide alternative plant-based formulations with greater stability and antibacterial activity

Effect of Telenursing Training on Death Anxiety in Nurses with a History of COVID-19

Introduction: In light of the global spread of COVID-19 and its profound impact on public health and casualties, nurses have been thrust onto the front lines in the battle against this disease, resulting in heightened psychological distress and anxiety. Addressing these issues promptly and effectively is crucial during these challenging times. Therefore, this study aims to investigate the impact of telenursing training in reducing death anxiety among nurses with a history of COVID-19. Material and Methods: This quasi-experimental study involved two groups of 20 nurses with a history of COVID-19 and higher levels of death anxiety. Data were collected using Templer's death anxiety questionnaire and a demographic information questionnaire. In the test group, the intervention was conducted through WhatsApp groups over five sessions. Training methods to reduce death anxiety were presented through explanatory text, PowerPoint presentations, and audio files, with five-day intervals between sessions. The control group did not receive any intervention. Twenty days after the sessions, both groups completed the death anxiety questionnaire again. Data were analyzed using t-tests and chi-square tests. Results: The findings indicated a significant difference in the average score of death anxiety between the test and control group after telenursing training (p<0.05). Conclusion: Telenursing training effectively reduces death anxiety among nurses with a history of COVID-19. Telenursing proves to be a cost-effective and organized intervention for managing symptoms, early diagnosis of complications, ensuring post-care quality, exchanging information, and providing health education

Carbon nanoparticle-based COVID-19 biosensors

Coronavirus disease (COVID-19) is a new emerged contagious human-to-human infection that broke out in early December 2019, threatens global public health and causing widespread concern. The high lethality and transmission power of this virus introduce it as a dangerous factor and multiplies the importance of its rapid diagnosis. Tests like computerized tomography (CT) scan, and poly-chain reaction (PCR), were very popular at the beginning of the pandemic, But over time, taking into account the high rate of transmission of the disease, the need for fast and cost-effective diagnostic tests with significant sensitivity and specificity such as clustered regularly interspaced short palindromic repeats (CRISPR), Enzyme-Linked Immunosorbent Assay (ELISA), lateral flow assay (LFA), and biosensor was felt more. In this context, there is a global interest in the feasibility of employing nano-biosensors, especially those using carbon and its derivatives as a key material, for the real-time virus detection. The exceptional properties of carbon and the outstanding performance of nano-biosensors in identifying various viruses prompted a feasibility check on this technology. In this article, we have tried to introduce several carbon-based nano biosensors with various limits of detection (LOD) and different characteristics that have been used in identifying and limiting the spread of Covid-19

Recent Advances in Nanotechnology for the Management of <i>Klebsiella pneumoniae</i>–Related Infections

Klebsiella pneumoniae is an important human pathogen that causes diseases such as urinary tract infections, pneumonia, bloodstream infections, bacteremia, and sepsis. The rise of multidrug-resistant strains has severely limited the available treatments for K. pneumoniae infections. On the other hand, K. pneumoniae activity (and related infections) urgently requires improved management strategies. A growing number of medical applications are using nanotechnology, which uses materials with atomic or molecular dimensions, to diagnose, eliminate, or reduce the activity of different infections. In this review, we start with the traditional treatment and detection method for K. pneumoniae and then concentrate on selected studies (2015–2022) that investigated the application of nanoparticles separately and in combination with other techniques against K. pneumoniae

Polyacrylic Acid Nanoplatforms: Antimicrobial, Tissue Engineering, and Cancer Theranostic Applications

Polyacrylic acid (PAA) is a non-toxic, biocompatible, and biodegradable polymer that gained lots of interest in recent years. PAA nano-derivatives can be obtained by chemical modification of carboxyl groups with superior chemical properties in comparison to unmodified PAA. For example, nano-particles produced from PAA derivatives can be used to deliver drugs due to their stability and biocompatibility. PAA and its nanoconjugates could also be regarded as stimuli-responsive platforms that make them ideal for drug delivery and antimicrobial applications. These properties make PAA a good candidate for conventional and novel drug carrier systems. Here, we started with synthesis approaches, structure characteristics, and other architectures of PAA nanoplatforms. Then, different conjugations of PAA/nanostructures and their potential in various fields of nanomedicine such as antimicrobial, anticancer, imaging, biosensor, and tissue engineering were discussed. Finally, biocompatibility and challenges of PAA nanoplatforms were highlighted. This review will provide fundamental knowledge and current information connected to the PAA nanoplatforms and their applications in biological fields for a broad audience of researchers, engineers, and newcomers. In this light, PAA nanoplatforms could have great potential for the research and development of new nano vaccines and nano drugs in the future

Synthesize of pluronic-based nanovesicular formulation loaded with Pistacia atlantica extract for improved antimicrobial efficiency

One of the current concerns to human health is antibiotic resistance, which promotes the use of antibiotics that are more harmful, expensive, and ineffective. In this condition, researchers are turning to innovative options to combat this alarming situation. Combining herbal medicine with nanotechnology has created a new strategy to increase the effectiveness of phytochemical compounds in overcoming antimicrobial resistance. Pistacia atlantica is one of the promising herbs with medicinal benefits, but its poor solubility in biological fluids is challenging. In this regard, we seek to evaluate the antibacterial efficacy of Pistacia atlantica extract-loaded nanovesicle. Cholesterol, Span 40, and Pluronic F127 modified nanoformulation was developed using an environmentally friendly improved heating technique, and it was evaluated for size distribution, zeta potential, morphology, entrapment efficiency (EE%), release behavior, stability, and antimicrobial performance. By using DLS, spherical nanovesicles were identified with a size distribution of 50–150 nm and a zeta potential of −43 mV. The extract's encapsulation efficiency was 72.03%. The developed loaded nanovesicles demonstrated controlled extract release in the tested 96 h and storage stability of at least 12 months at 25 °C. Also, Comparing the two samples, the encapsulated extract had greater antibacterial activity against Candida albicans, Staphylococcus aureus, and Pseudomonas plecoglossicida with MIC of 1320, 570, and 1100 µg/mL, respectively. Besides reducing the misuse of antibiotics by allowing for the controlled release of drugs made from natural sources, we expect the findings described here to help provide alternative plant-based formulations with greater stability and antibacterial activity