19 research outputs found
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle - Fig 7
<p>Relative mRNA abundance of genes affected by Side (antimesometrial <i>vs</i>. mesometrial; Panel A; arbitrary units: AU; mean ± SEM) and by the Side by Region (IA, IM <i>vs</i>. IP; Panel B; arbitrary units: AU; mean ± SEM) interaction in the uterine horn ipsilateral to the CL. *<i>P</i> < 0.05 and ˟<i>P</i> < 0.1 denotes that significant differences were reached or approached, respectively, between sides at each specific region.</p
Effects of Group (Preg <i>vs</i>. Con), Region (UTJ, IA, IM <i>vs</i>. IP) and Group by Region in the abundance of transcripts showing significant Group by Region (<i>G*R)</i> interaction.
<p>Effects of Group (Preg <i>vs</i>. Con), Region (UTJ, IA, IM <i>vs</i>. IP) and Group by Region in the abundance of transcripts showing significant Group by Region (<i>G*R)</i> interaction.</p
Relative mRNA abundance of eicosanoid biosynthesis related genes (arbitrary units; AU; mean ± SEM) for control (solid lines) and pregnant (dashed lines) cows in the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL.
<p>*<i>P</i> < 0.05 and Ëź<i>P</i> < 0.1 denotes that significant differences were reached or approached, respectively, between groups at each specific region.</p
Relative mRNA abundance of interferon-stimulated genes (arbitrary units; AU; mean ± SEM) for control (solid lines) and pregnant (dashed lines) cows in the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL.
<p>*<i>P</i> < 0.05 and Ëź<i>P</i> < 0.1 denotes that significant differences were reached or approached, respectively, between groups at each specific region.</p
Size of ovarian structures and P4 concentrations in pregnant (Preg) and sham inseminated (Con) cows.
<p>Size of ovarian structures and P4 concentrations in pregnant (Preg) and sham inseminated (Con) cows.</p
Schematic representation of sites selected for collection of endometrial samples.
<p>Panel A: Endometrial tissue samples were collected from 8 regions of the uterus: uterotubal junction (UTJ) ipsi and contralateral to the ovary containing the CL and the intermediate portion of the anterior, medial and posterior thirds of the ipsilateral uterine horn, that were further divided in mesometrial (M) and antimesometrial (A) sides. Panel B: Representative cross-section of the uterine horn indicating the sites for endometrium collection according to the mesometrium insertion.</p
Experimental design.
<p>The estrous cycles of Nelore cows (n = 36) were synchronized using an 8-day progesterone-releasing intravaginal device. On day –10 (D–10), cows received a progesterone-releasing device (1 g; Sincrogest; Ourofino) and an injection of 2 mg estradiol benzoate (EB; Sincrodiol, Ourofino) and an injection of prostaglandin F2α (PGF2α; 500 μg of sodium cloprostenol; Sincrocio, Ourofino). On D–3, when the progesterone-releasing device was removed, all cows received an extra injection of PGF2α. At estrus (D0), cows were allocated to one of two experimental groups: Control (Con), cows were sham-inseminated and received semen extender; Pregnant (Preg), cows were inseminated with semen from the same batch of semen from a bull of proven fertility, 12 h post-estrus. All cows were slaughtered on D7.</p
Representative genes selected from key pathways known to influence endometrial function and endogenous controls.
<p>Representative genes selected from key pathways known to influence endometrial function and endogenous controls.</p
Summary of side-dependent differences in gene expression.
<p>Schematic representation of distribution of genes affected by Side (antimesometrial <i>vs</i>. mesometrial; Panel A) and by the Side by Region (IA, anterior; IM, medial; IP, posterior; Panel B) interaction in the uterine horn ipsilateral to the CL. The position of each differentially expressed gene in the figure indicate an upregulation in the side.</p
Genes representing clusters shown in Fig 6.
<p>Genes representing clusters shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175954#pone.0175954.g006" target="_blank">Fig 6</a>.</p