Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

<p>Abstract</p> <p>Background</p> <p>Two sets of overlapping genes, <it>lacLMReu </it>and <it>lacLMAci</it>, encoding heterodimeric β-galactosidases from <it>Lactobacillus reuteri </it>and <it>Lactobacillus acidophilus</it>, respectively, have previously been cloned and expressed using the pSIP vector system and <it>Lactobacillus plantarum </it>WCSF1 as host. Despite the high similarity between these <it>lacLM </it>genes and the use of identical cloning and expression strategies, strains harboring <it>lacLMReu </it>produced about twenty-fold more β-galactosidase than strains containing <it>lacLMAci</it>.</p> <p>Results</p> <p>In this study, the plasmid copy numbers (PCN) of expression vectors pEH9R (<it>lacLMReu</it>) and pEH9A (<it>lacLMAci</it>) as well as the transcription levels of both <it>lacLM </it>genes were compared using quantitative PCR methods. Analyses of parallel fermentations of <it>L. plantarum </it>harboring either pEH9R or pEH9A showed that the expression plasmids were present in similar copy numbers. However, transcript levels of <it>lacLM </it>from <it>L. reuteri </it>(pEH9R) were up to 18 times higher than those of <it>lacLM </it>from <it>L. acidophilus </it>(pEH9A). As a control, it was shown that the expression levels of regulatory genes involved in pheromone-induced promoter activation were similar in both strains.</p> <p>Conclusion</p> <p>The use of identical expression strategies for highly similar genes led to very different mRNA levels. The data indicate that this difference is primarily caused by translational effects that are likely to affect both mRNA synthesis rates and mRNA stability. These translational effects thus seem to be a dominant determinant for the success of gene expression efforts in lactobacilli.</p

Triple Michelson Interferometer for a Third-Generation Gravitational Wave Detector

The upcoming European design study `Einstein gravitational-wave Telescope' represents the first step towards a substantial, international effort for the design of a third-generation interferometric gravitational wave detector. It is generally believed that third-generation instruments might not be installed into existing infrastructures but will provoke a new search for optimal detector sites. Consequently, the detector design could be subject to fewer constraints than the on-going design of the second generation instruments. In particular, it will be prudent to investigate alternatives to the traditional L-shaped Michelson interferometer. In this article, we review an old proposal to use three Michelson interferometers in a triangular configuration. We use this example of a triple Michelson interferometer to clarify the terminology and will put this idea into the context of more recent research on interferometer technologies. Furthermore the benefits of a triangular detector will be used to motivate this design as a good starting point for a more detailed research effort towards a third-generation gravitational wave detector.Comment: Minor corrections to the main text and two additional appendices. 14 pages, 6 figure

Removing non-stationary, non-harmonic external interference from gravitational wave interferometer data

We describe a procedure to identify and remove a class of non-stationary and non-harmonic interference lines from gravitational wave interferometer data. These lines appear to be associated with the external electricity main supply, but their amplitudes are non-stationary and they do not appear at harmonics of the fundamental supply frequency. We find an empirical model able to represent coherently all the non-harmonic lines we have found in the power spectrum, in terms of an assumed reference signal of the primary supply input signal. If this signal is not available then it can be reconstructed from the same data by making use of the coherent line removal algorithm that we have described elsewhere. All these lines are broadened by frequency changes of the supply signal, and they corrupt significant frequency ranges of the power spectrum. The physical process that generates this interference is so far unknown, but it is highly non-linear and non-stationary. Using our model, we cancel the interference in the time domain by an adaptive procedure that should work regardless of the source of the primary interference. We have applied the method to laser interferometer data from the Glasgow prototype detector, where all the features we describe in this paper were observed. The algorithm has been tuned in such a way that the entire series of wide lines corresponding to the electrical interference are removed, leaving the spectrum clean enough to detect signals previously masked by them. Single-line signals buried in the interference can be recovered with at least 75 % of their original signal amplitude.Comment: 14 pages, 5 figures, Revtex, psfi

Gravitational-wave research as an emerging field in the Max Planck Society. The long roots of GEO600 and of the Albert Einstein Institute

On the occasion of the 50th anniversary since the beginning of the search for gravitational waves at the Max Planck Society, and in coincidence with the 25th anniversary of the foundation of the Albert Einstein Institute, we explore the interplay between the renaissance of general relativity and the advent of relativistic astrophysics following the German early involvement in gravitational-wave research, to the point when gravitational-wave detection became established by the appearance of full-scale detectors and international collaborations. On the background of the spectacular astrophysical discoveries of the 1960s and the growing role of relativistic astrophysics, Ludwig Biermann and his collaborators at the Max Planck Institute for Astrophysics in Munich became deeply involved in research related to such new horizons. At the end of the 1960s, Joseph Weber's announcements claiming detection of gravitational waves sparked the decisive entry of this group into the field, in parallel with the appointment of the renowned relativist Juergen Ehlers. The Munich area group of Max Planck institutes provided the fertile ground for acquiring a leading position in the 1970s, facilitating the experimental transition from resonant bars towards laser interferometry and its innovation at increasingly large scales, eventually moving to a dedicated site in Hannover in the early 1990s. The Hannover group emphasized perfecting experimental systems at pilot scales, and never developed a full-sized detector, rather joining the LIGO Scientific Collaboration at the end of the century. In parallel, the Max Planck Institute for Gravitational Physics (Albert Einstein Institute) had been founded in Potsdam, and both sites, in Hannover and Potsdam, became a unified entity in the early 2000s and were central contributors to the first detection of gravitational waves in 2015.Comment: 94 pages. Enlarged version including new results from further archival research. A previous version appears as a chapter in the volume The Renaissance of General Relativity in Context, edited by A. Blum, R. Lalli and J. Renn (Boston: Birkhauser, 2020