Additional file 2: of Comprehensive metabolic profiling of chronic low-grade inflammation among generally healthy individuals

Supplemental Tables. (XLSX 30 kb

Additional file 1: of Comprehensive metabolic profiling of chronic low-grade inflammation among generally healthy individuals

Supplemental Matrial, Methods and Figures. (DOCX 678 kb

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

02 septembre 18751875/09/02 (A9,N3404)

General characteristics of the study population.

<p>General characteristics of the study population.</p

Corrected p-values (false discovery rate; FDR) from linear regression analyses with osteocalcin concentration as exposure and plasma (left upper panel) or urine (right lower panel) metabolites as outcomes.

<p>Models were adjusted for age, sex, waist circumference and physical activity. The upper right panel contains results for metabolites present in both fluids. Within each panel the dotted lines denote the significance threshold of FDR<0.05. Metabolites are colored if they are significantly associated in plasma (blue), urine (orange) or in both (red), respectively. Metabolites marked with a diamond exceed the plotting range. Metabolites highlighted by increased point size and darker colors persisted significant even after further adjustment for hormone intake, estimated glomerular filtration rate and smoking behavior. Corresponding beta estimates and FDR-values can be found in supplemental Tables <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184721#pone.0184721.t001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184721#pone.0184721.t002" target="_blank">2</a>. Metabolites numbered with 1 to 7 are named in the lower left panel. Triangles indicate the direction of the association in plasma or urine, with ▲ indicating positive associations.</p

Plasma and urine metabolites significantly associated with the serum osteocalcin concentration.

<p>Plasma and urine metabolites significantly associated with the serum osteocalcin concentration.</p

Cross-sectional associations of urinary metabolites with FT4 or TSH concentrations in both study populations.

<p>Cross-sectional associations of urinary metabolites with FT4 or TSH concentrations in both study populations.</p

Cross-sectional association between urine metabolome and thyroid function.

<p>A) Corrected p-values (false discovery rate (FDR)) of the cross-sectional associations of metabolites levels (right side) or ppm (left side) with free thyroxine (FT4) or thyrotropin (TSH) in Inter99 and Health2006/08. Multivariable linear regression models were adjusted for age, sex, body-mass-index, HbA1c, low-density lipoprotein cholesterol and systolic blood pressure. B) Median levels of alanine, lactate, N,N-dimethylglycine or trigonelline by tertiles of FT4 in Inter99 and Health2006/08. C): Median levels of glycine, lactate, dimethylamine or glucose by tertiles of log(TSH) in Inter99 and Health2006/08.</p

Longitudinal associations of urinary metabolites with changes in FT4 or TSH concentrations in Inter99.

<p>Longitudinal associations of urinary metabolites with changes in FT4 or TSH concentrations in Inter99.</p

Longitudinal association between urine metabolome and thyroid function.

<p>A) Corrected p-values (false discovery rate (FDR)) of the longitudinal associations of urine metabolites levels (right side) or ppm (left side) with changes in free thyroxine (FT4) or thyrotropin (TSH) in Inter99. Models were performed in all subjects (x-axis) and *only in subjects with noticeable change assessed by the reference change value (RCV). Multivariable mixed linear models were adjusted for age, sex, body-mass-index, HbA1c, low-density lipoprotein cholesterol and systolic blood pressure (for more details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173078#sec006" target="_blank">method</a> section). Estimated mean value with 95% confidence limits for changes in free thyroxine (B: ΔFT4) or thyrotropin (C: Δlog(TSH)) by quartiles of selected urinary metabolite concentrations in Inter99 calculated by mixed linear models. DMA = dimethylamine. DMG = dimethylglycine.</p