Genotypic and allelic comparisons between Typhoid cases and controls.

1<p>ND = not done.</p

Summary of TLR4 polymorphisms associated with infectious diseases.

<p>Summary of TLR4 polymorphisms associated with infectious diseases.</p

Mutation detection in the T4_promoter fragment.

<p>Three polymorphisms were detected by dHPLC in the T4_promoter fragment and were verified by DNA sequencing. The four dHPLC graphs correspond to the wild-type sequence (A), SNPs T-441C (B), SNP A-271G (C) and SNP G-259C (D). The wild-type pattern is visible in all dHPLC graphs as a 1 peak trace. SNP T-441C is identified by an unequal height 2 peak trace (B), SNP A-271G is identified by an equal height 2 peak trace (C) and SNP G-259C is identified by a 3 peak trace (D). The positions of the sequence variants in the T4_promoter fragment identified by dHPLC were determined by DNA sequencing. All SNPs were present in the heterozygous state.</p

The positions of the <i>TLR4</i> polymorphisms in the genomic sequence and the polypeptide sequence.

<p>Three polymorphisms were identified in the upstream region (T-441C, A-271G, G-259C), one in intron 2 (T4025A), one in exon 2 (C4215G), and five in exon 3 (C7944T, A7947G, A8177G, C8850T, G9605T). E1, E2, E3, E2a, represent exons 1, 2, 3 and the alternative exon 2. Transcriptional site indicated as +1. 6 exonic polymorphisms cause a change in amino acid residue, with 5 in the ectoplasmic domain and 1 in the plasma membrane domain. LRR denotes; leucine rich repeat. TIR denotes; Toll/IL-1R domain.</p

Primers used to generate amplicons for mutation detection in <i>TLR4</i> by dHPLC.

1<p>nucleotides in bold represent GC clamps added to the primer to improve the melting profile of the generated amplicon for mutation detection.</p>2<p>the temperatures used in dHPLC for each fragment to accurately identify sequence changes in TLR4.</p

<i>TLR4</i> gene structure and fragment design for dHPLC.

<p>E1, E2, E3 denotes exons 1, 2, and 3. E2a denotes an alternative exon 2. I1, I2, I3 denotes intronic sequences. 5′UTR and 3′UTR represent the untranslated regions. The lines underneath the gene structure show the approximate positions of the 11 fragments designed for mutation detection by dHPLC. The names of each fragment are above the lines.</p