1 research outputs found
Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in <i>Drosophila</i> Brain
Time-of-flight secondary
ion mass spectrometry (ToF-SIMS) was used
to study the effects of cocaine versus methylphenidate administration
on both the localization and abundance of lipids in <i>Drosophila
melanogaster</i> brain. A J105 ToF-SIMS with a 40 keV gas cluster
primary ion source enabled us to probe molecular ions of biomolecules
on the fly with a spatial resolution of ∼3 μm, giving
us unique insights into the effect of these drugs on molecular lipids
in the nervous system. Significant changes in phospholipid composition
were observed in the central brain for both. Principal components
image analysis revealed that changes occurred mainly for phosphatidylcholines,
phosphatidylethanolamines, and phosphatidylinositols. When the lipid
changes caused by cocaine were compared with those induced by methylphenidate,
it was shown that these drugs exert opposite effects on the brain
lipid structure. We speculate that this might relate to the molecular
mechanism of cognition and memory