2 research outputs found
Investigating the diversity of resistant starch in Vietnamese rice collection
The rapid increase of obesity and type 2 diabetes has recently posed an enormous burden on the healthcare system worldwide. Resistant starch (RS) in rice can escape digestion by enzymes in the small intestine, making its calories unavailable for cells to use. As a result, RS can be used by diabetes patients to prevent diabetes and for obese individuals who do not want extra energy. In our study, 75 Vietnamese rice accessions originating from diverse ecosystems were chosen as plant materials to investigate the diversity of RS content in this collection. The Megazyme kit was used to measure the amount of RS. The release of quinonimine was measured using a spectrophotometer at 510 nm. The results showed that approximately 70% of Vietnamese rice accessions had RS content ranging from 0.015 to 0.2% while only 4% of samples had RS content ranging from 0.6 to 0.8%. The Indica subgroups had significantly higher RS content than the Japonica subgroup. Higher RS content was found in medium- and short- grain rice rather than in long grain. Finally, rice plants grown in rainfed lowlands (RL) and irrigated ecosystems had higher RS content than those grown under mangrove and upland ecosystems. Our results firstly give information about the diversity of RS in Vietnamese rice and secondly may contribute to the field of nutrition by developing a suitable rice-based diet for patients with diabetes or obesity
Widely targeted metabolomics reveals the species‐specific, matureness‐specific and post‐harvest‐specific discriminations in the chemical profiles of Vietnamese endemic golden camellias
International audienceSummary Vietnam is one of the countries with the highest diversity of golden camellias in the world, with more than forty endemic species. However, studies on Vietnamese golden camellias need to be expanded to fill a knowledge gap on these species' chemical profiles and pharmacological properties. This qualitative study focuses on six golden camellias: four endemic species from Tam Dao district, Vinh Phuc province, including Camellia hakodae , Camellia phanii , Camellia tamdaoensis , Camellia tienii and two species commonly cultivated in Quang Ninh province including Camellia petelotii and Camellia euphlebia . A mutual profile of 131 metabolites was obtained by using widely targeted metabolomics analysis using ultra‐performance liquid chromatography coupled with tandem mass spectrometry. Comparative metabolomics was performed among leaf extracts of six golden teas, between young and mature leaves and between freeze‐drying and oven‐drying leaves to discriminate these species, and determine the effect of developmental stage and post‐harvesting method on the plant metabolism. The PLS‐DA model successfully illustrated a clear separation of the six species and suggested apigenin C‐glycoside derivatives as chemical markers for the diversity. Young and mature leaves of all six species also produced distinguished metabolomes, differentiated by mostly amino acid derivatives. The multivariate analysis also indicated the conversion of some flavonoids and amino acids induced by heat in the drying process. These results offer metabolite markers for quality control of Vietnamese golden camellias and recommendations for their usage and preparation