A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles

WOS: 000286680900015PubMed: 21176840The aim of this study is to prepare supermacroporous cryogels embedded with Cu2+-attached sporopollenin particles (Cu2+-ASP) having large surface area for high protein adsorption capacity. Super-macroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Cu2+-ASP was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA). Firstly, Cu2+ ions were attached to sporopollenin particles (SP), then the supermacroporous PHEMA cryogel with embedded Cu2+-ASP was produced by free radical polymerization using N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Embedded particles (10 mg) in PHEMA-based cryogel column were used in the adsorption/desorption of HSA from aqueous solutions. Optimum conditions of adsorption experiments were performed at pH 8.0 phosphate buffer, with flow rate of 0.5 mL/min, and at 5 degrees C. The maximum amount of HSA adsorption from aqueous solution was very high (677.4 mg/g SP) with initial concentration 6 mg/mL It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Cu2+-ASP in PHEMA cryogel without significant loss of adsorption capacity. (C) 2010 Elsevier B.V. All rights reserved

Yüksek miyop hastalarda fakoemulsifikasyon ve intraokuler lens implantasyonun klinik sonuçları

WOS: 000364167600004Objective: To evaluate the clinical outcomes of phacoemulsification and intraocular lens (IOL) implantation in eyes with high myopia. Material and Methods: This retrospective study included 52 eyes of 35 patients with an axial length greater than 26 mm who had undergone phacoemulsification and IOL implantation surgery. Patients who had ocular pathologies other than high myopia and cataract were excluded from this study. All patients' charts were reviewed, and preoperative and postoperative best corrected visual acuity (BCVA), refraction error values, and complications were recorded. Results: The mean age of the patients was 58.06 +/- 15.46 years (range 27 to 80 years), and the mean follow up time was 8.44 +/- 3.62 months. The mean preoperatively myopia was -10.94 +/- 4.75 diopters (D), mean axial length was 28.14 +/- 1.72 mm and the mean BCVA was 0.84 +/- 0.4 logMAR. Intraoperative vitreous loss occurred in two (3.85%) eyes, and a posterior chamber IOL was implanted in the sulcus in these cases. In 8 (15.4%) eyes, peripheral retinal degenerations detected and these patients underwent prophylactic argon laser treatment preoperatively. The mean postoperative spheric value was -0,26 +/- 1.51 D and, the mean BCVA was 0.24 +/- 0.24 logMAR. There was a significant decrease in the myopia values (Wilcoxon testi p<0.001) and a significant increase in the BCVA (Wilcoxon testi p<0.001). During the follow up time, no posterior segment complications were detected. Conclusion: Phacoemulsification surgery is a safe and effective method with successful anatomical and functional outcomes in patients with high myopia. Detailed retinal examinations and prophylactic laser photocoagulation applications decrease retinal complication rates.Amaç: Yüksek miyop hastalarda fakoemulsifikasyon ve intraokuler lens implantasyon cerrahisinin klinik sonuçlarının değerlendirilmesi. Gereç ve Yöntem: Bu retrospektif çalışmaya katarakt nedeniyle fakoemulsifikasyon ve intraokuler lens implantasyonu cerrahisi uygulanan, aksiyel uzunluğu 26 mm’den fazla olan 35 hastanın 52 gözü dahil edildi. Katarakt ve yüksek miyopi dışında başka bir okuler patolojisi olan hastalar çalışma kapsamı dışında bırakıldı. Hastalara ait dosyalar taranarak, ameliyat öncesi ve sonrası en iyi düzeltilmiş görme keskinliği (EİDGK), refraksiyon kusurları ve komplikasyonlar kayıt edildi. Bulgular: Çalışmaya alınan hastaların ortalama yaşı 58.06±15.46 yıl (27-80 yıl) ve ortalama takip süresi 8.44±3.62 ay idi. Ortalama preoperatif miyopi -10.94±4.75 diyoptri (D), ortalama aksiyel uzunluğu 28.14±1.72 mm ve ortalama EİDGK 0.84±0.4 logMAR idi. İntraoperatif vitreus kaybı iki hastada (3.85%) saptandı ve bu hastalara, arka kamara lensi sulkusa implante edildi. Periferik retina dejenerasyonları saptanan 8 göze (15.4%) preoperatif profilaktik argon lazer uygulaması yapıldı. Ortalama postoperatif sferik değer -0.26±1.51 D ve ortalama EİDGK 0.24±0.24 logMAR idi. Miyopi değerinde istatistiksel anlamlı düşüş (Wilcoxon testi p<0.001) ve EİDGK değerinde istatistiksel anlamlı artış (Wilcoxon testi p<0.001) saptandı. Sonuç: Yüksek miyop hastalarda fakoemulsifikasyon cerrahisi anatomik ve fonksiyonel olarak başarılı sonuçları ile güvenli ve etkili bir yöntemdir. Detaylı retina muayenesi ve profilaktik uygulanan fotokoagulasyon işlemi retinal komplikasyonların oranının azaltılmasını sağlayabilir

A rare complication after septoplasty: Visual loss due to right retinal artery spasm

WOS: 000427989400084PubMed ID: 29239920Septoplasty is a commonly used procedure for correcting septal cartilage deformities. Hemorrhage, abscesses, scaling, adhesions, and scar tissue are often seen after the operation of the septoplasty, but temporary or permanent visual loss due to local anesthetic use has been reported very rarely in the literature. The authors also aimed to present a female patient with retinal artery spasm in the right eye after septoplasty in this article. A 27-year-old female patient was admitted to the authors' clinic with long-standing nasal obstruction and postnasal drip. There was no feature in her history and also no sign other than nasal septal deviation on physical examination. The patient was informed about the operation and the operation was planned. Emergency eye consultation was requested after the patient said that the right eye of the patient had never seen in the postoperative wake-up hall. Examination by an ophthalmologist; mild exotropia and total loss of vision in the right eye (including loss of light reflex) was detected. The light reaction in the affected eye was negative and indirect reaction was positive. After enlargement of the pupil, fundus examination revealed that the right posterior pole region (inside of the macula and vessel arches) was pale and no central retinal artery pulsation was observed. The patient was diagnosed with central retinal artery occlusion and emergency intervention was performed. The right eye massage, paracentesis, and hyperbaric oxygen therapy returned to the patient's visual function

A novel matrix for hydrophobic interaction chromatography and its application in lysozyme adsorption

PubMed ID: 25298972A novel 1-naphthylamine (NA) coupled poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(L)-histidine methyl ester) [NA-PHEMAH] supermacroporous monolithic hydrophobic cryogel was prepared via covalent coupling of NA to PHEMAH for adsorption of lysozyme from aqueous solution. Firstly, PHEMAH monolithic cryogel was prepared by radical cryocopolymerization of HEMA with MAH as a functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as a crosslinker directly in a plastic syringe, and then NA molecules were covalently attached to the imidazole rings of MAH groups of the polymeric structure. The prepared, NA-PHEMAH, supermacroporous monolithic hydrophobic cryogel was characterized by scanning electron microscopy (SEM). The effects of initial lysozyme concentration, pH, salt type, temperature and flow rate on the adsorption efficiency of monolithic hydrophobic cryogel were studied in a column system. The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 86.1 mg/g polymer at pH 8.0 with a flow rate of 1 mL/min. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the NA-PHEMAH monolithic hydrophobic cryogel without significant loss of the adsorption capacity

Investigation of protein adsorption performance of Ni2+-attached diatomite particles embedded in composite monolithic cryogels

WOS: 000295189400027PubMed: 21739602As a low-cost natural adsorbent, diatomite (DA) (2 mu m) has several advantages including high surface area, chemical reactivity, hydrophilicity and lack of toxicity. In this study, the protein adsorption performance of supermacroporous composite cryogels embedded with Ni2+-attached DA particles (Ni2+-ADAPs) was investigated. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic composite cryogel column embedded with Ni2+-ADAPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as crosslinker directly in a plastic syringe for affinity purification of human serum albumin (H SA) both from aqueous solutions and human serum. The chemical composition and surface area of DA was determined by XRF and BET method, respectively. The characterization of composite cryogel was investigated by SEM. The effect of pH, and embedded Ni2+-ADAPs amount, initial HSA concentration, temperature and flow rate on adsorption were studied. The maximum amount of HSA adsorption from aqueous solution at pH 8.0 phosphate buffer was very high (485.15 mg/g DA). It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Ni2+-ADAPs in poly(2-hydroxyethyl methacrylate) composite cryogel without significant loss of adsorption capacity. The efficiency of albumin adsorption from human serum before and after albumin adsorption was also investigated with SDS-PAGE analyses

Monolithic hydrophobic cryogel columns for protein separation

*Erzengin, Mahmut ( Aksaray, Yazar ) *Odabaşı, Mehmet ( Aksaray, Yazar )The present study was conducted for the synthesis of a novel supermacroporous monolithic hydrophobic adsorbent for lysozyme (Lyz) selected as a model protein from aqueous solution. After preparation of poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(l)-tyrosine methyl ester) monolithic cryogel column, 1-naphthylamine was covalently attached, and the prepared column was abbreviated as NA-Mcc. Scanning electron microscopy, Fourier transform infrared spectroscopy and Brunauer, Emmett and Teller device were utilized for the morphology, functional groups and surface area measurements of the column. Effects of several parameters including Lyz content of the adsorption solution, pH of the medium used, ambient temperature, type of salt and flow rate on the adsorption capacity of the polymeric material were examined in continuous operation. The maximum value achieved for Lyz adsorption from aqueous phase was found to be 105.8 mg/g in phosphate buffer. In addition, NA-Mcc was investigated in terms of reusability, and it was demonstrated that there is no significant change in the adsorption properties of prepared monolithic hydrophobic cryogels after 30 adsorption-desorption cycles

Investigation of demodex parasite existence in treatment-resistant chronic blepharitis cases

Amaç: Demodex akarı, zorunlu bir ektoparazit olup, insanda özellikle kıl folikülleri, kirpik kökleri ve yağ salgı bezlerine yerleşmektedir. Bu çalışmanın amacı tedaviye dirençli kronik blefaritli olgularda Demodex enfestasyonu varlığını araştırmaktır. Yöntemler: Çalışmaya İstanbul Medipol Üniversitesi Tıp Fakültesi Hastanesi Göz Hastalıkları Polikliniği’ne başvuran kronik blefarit tanısı alan olgular dahil edildi. Tüm olgular daha önce en az üç kez blefarit tedavisi almış idi. Hastalardan blefaritin yoğun olduğu alanda bulunan kirpiklerinden 3-4 adet örnek alınarak ışık mikroskobu altında incelendi. Işık mikroskobunda 10x ve 40x büyütmede incelenen alanda bir veya daha fazla Demodex parazitinin görülmesi pozitif olarak kabul edildi. Bulgular: Çalışmaya 153 olgu dahil edildi. Olguların 79’u (%51,6) erkek, 74’ü (%48,4) kadın idi. Ortalama yaş 43,1±9,7 olarak bulundu. Işık mikroskopisi ile yapılan inceleme sonucunda 153 kronik blefaritli olgunun 69’unda (%45,1) kirpik foliküllerinde Demodex paraziti varlığı tespit edildi. Sonuç: Özellikle tedaviye dirençli kronik blefaritli olgularda Demodex enfestasyonunun mutlaka göz önüne alınması gerektiğini, parazitin tedavi öncesinde rutin olarak aranmasının faydalı olacağını düşünmekteyiz.OBJECTIVE: Demodex acari is an obligate ectoparasite, and it is usually located in the human hair follicles, eyelash roots, and sebaceous glands. The aim of this study was to investigate the presence of Demodex infestation in chronic blepharitis cases that are resistant to therapy. METHODS: Patients who were admitted at the Istanbul Medipol University School of Medicine Hospital with a diagnosis of chronic blepharitis were included. All cases received conventional therapy at least three times. Three or four eyelash samples from patients with blepharitis were collected and examined under light microscopy. For the diagnosis, the presence of one or more Demodex parasites at 10× and 40× magnification by a light microscope was considered as positive for infestation. RESULTS: Overall, 153 cases were included in the study. Of the cases, 79 (51.6%) were males and 74 (48.4%) were females. The mean age was 43.1±9.7 years. The presence of D. acari in the follicles of the eyelashes in patients with chronic blepharitis was found in 69 (45.1%) cases. CONCLUSION: D. acari should be considered in patients with chronic blepharitis, especially in treatment-resistant cases. We believe that it would be useful to search for the parasite in patients with blepharitis prior to treatment on a routine basis

Effect of Ultrasound Time on Macular Thickness and Visual Acuity in Cataract Surgery

Pur po se: The aim of this study was to investigate the effect of ultrasound time on macular thickness and the influence of macular thickness on visual acuity after uneventful phacoemulsification surgery. Ma te ri al and Met hod: In this study, 42 eyes of 30 cataract patients who underwent uncomplicated phacoemulsification surgery were evaluated. The phacoemulsification was performed using 20% “pulse mode” ultrasound energy. The ultrasound time of each case was noted. Visual acuity (logMAR) and macular thickness were measured pre- and postoperatively. Macular thickness was measured by “spectraldomain” optical coherence tomography using “macular map 6” (MM6) programme. Re sults: All patients who underwent uncomplicated phacoemulsification surgery showed a statistically significant improvement in bestcorrected visual acuity in postoperative first and third months (p=0.001). When minimum central macular, mean central zone, mean parafoveal and mean perifoveal macular thicknesses in the postoperative first week, first month and third month were compared with the preoperative values, a statistically significant increase in all zones was detected (p=0.001). The mean ultrasound time applied was 128 sec. ±11 SD: 70.17. No statistically significant correlation was found between ultrasound time and increase in macular thickness in the postoperative first week, first month and third month. There was no significant correlation between best-corrected visual acuity and minimum central macular/central zone macular thickness. Dis cus si on: This study shows that in patients who underwent uneventful phacoemulsification surgery, there is an increase in macular thickness independent of ultrasound time. We also found no correlation between macular thickness and visual acuity. (Turk J Ophthalmol 2011; 41: 286-90

Kombine fakovitrektomi cerrahisi sonrası ön kamara derinliğindeki değişiklikler

Objectives: To evaluate changes in anterior chamber depth (ACD) and postoperative refractive outcomes after combined phacovitrectomy.Materials and Methods: This study included 10 eyes of 10 patients that underwent combined phacovitrectomy (study group) and 14 eyes of 14 patients that underwent phacoemulsification surgery (control group) at İstanbul Medipol University Ophthalmology Department. Preoperative and 3-month postoperative best corrected visual acuity (BCVA), ACD, change in ACD and refractive outcomes were compared between the two groups.Results: Preoperative ACD, postoperative ACD at 3 months and change in ACD were similar between two groups (p=0.403, p=0.886, p=0.841). Postoperative mean refractive outcomes were 0.22&plusmn;0.51 diopter in the phacovitrectomy group and -0.39&plusmn;0.53 diopter in the phacoemulsification group (p=0.019). BCVA was increased in both groups (p=0.001).Conclusion: Postoperative refractive outcomes in eyes that underwent combined phacovitrectomy are different from those in eyes that underwent only phacoemulsification surgery. This is important in determining preoperative intraocular lens power before combined phacovitrectomy.Amaç: Fakovitrektomi cerrahisi sonrasında ön kamara derinliğinde (ÖKD) meydana gelen değişiklikleri ve refraktif sonuçları değerlendirmektir.Gereç ve Yöntem: İstanbul Medipol Üniversitesi Göz Hastalıkları Kliniği'nde fakovitrektomi yapılan 10 hastanın 10 gözü (çalışma grubu) ve fakoemülsifikasyon cerrahisi yapılan 14 hastanın 14 gözü (kontrol grubu) çalışmaya dahil edildi. İki grup arasında en iyi düzeltilmiş görme keskinliği (EİDGK), preoperatif ve postoperatif 3. ayda ÖKD, ÖKD'deki değişim ve refraktif kusurlar karşılaştırıldı. Bulgular: İki grup birbiriyle karşılaştırıldığı zaman preoperatif ÖKD, postoperatif 3. ay ÖKD ve ÖKD'deki değişim ölçümleri benzerdi (p=0,403, p=0,886, p=0,841). Postoperatif refraktif değerler (sferik eşdeğer) fakovitrektomi grubunda ortalama 0,22±0,51 diyoptri iken kontrol grubunda -0,39±0,53 diyoptri (p=0,019) idi. Her iki grupta da EDGK'da artış görüldü (p=0,001). Sonuç: Fakovitrektomi cerrahisi yapılan gözlerde refraktif sonuçlar sadece fakoemülsifikasyon yapılan gözlere göre daha farklı olmaktadır. Fakovitrektomi öncesi göz içi lensinin hesaplanmasında bu durum önemlidir