Using Rb-TnSeq to Analyze Natural Variation in Saccharomyces cerevisiae

One of the main challenges in biology today is the characterization of millions of genes of unknown function being continuously identified in sequencing studies. Transposon mutagenesis is a technique that has been widely used for annotating gene function and has now been combined with next-generation sequencing (Tn-Seq) to assess mutant fitness on a genome wide basis. However, Tn-Seq approaches are often constrained by laborious library preparation protocols which limit the number of organisms or conditions that can be assessed. Random bar code transposon-site sequencing (RB-TnSeq), is a transposon sequencing technique that streamlines library preparation and increases the throughput of mutant fitness profiling by incorporating random DNA barcodes into Tn5 Transposons. Rb-TnSeq has been successfully used for high throughput mutant fitness assays in diverse bacterial species. However, this technique is yet to be applied to a eukaryotic model organism. The goal of this study is to develop tools that allow the construction of barcoded mutant libraries in saccharomyces cerevisiae and describes methods for producing barcoded mutant libraries using a plasmid based or transposome based approach. These library construction protocols can prove to be powerful tools for studying gene function in S. cerevisiae on a genome wide basis

Portrayal of the President Asif Ali Zardari in Editorials: A Study of Pre and Post Presidential Election Coverage.

This research explores the image of ex-President Asif Ali Zardari in elite press before and after presidential election by comparing the coverage of daily “The Nation” and “Dawn”. For this study the time period has been chosen from June to 6th December 2008. For this empirical study the researcher explored the editorials of daily Nation and Dawn. In this empirical study the content analysis of the Nation and Dawn editorials have been analyzed. The editorials have been placed into 6 categories and it is linked with public opinion theory. This study help to unbderstand that how the media organization also in some cases disseminate their opinion during the high times of political event

Teaching the \u27comprehensive dental care\u27 in formative years of education and training: A new model for dental internship

There is a need of a new model of education and training to be implemented in the Bachelors of Dental Surgery curriculum in the relevant Pakistani institutions. The current review article was planned to suggest such a model in the light of literature aimed at building the capacity of dental graduates in a competency-driven approach with the objective of offering safe, efficient and comprehensive care to dental patients. The outcome of the reforms suggested shall prepare dental graduates suitably geared towards providing community-oriented family dental care right from their formative years. Moreover, the suggested internship model can also help to address the issue of inefficiency related to patient-care

What Are Your Children Watching? Teacher’s Evaluation of the Educational, Emotional, Behavioral, Psychological, Physical & Religious impacts of Cartoons on the School going Children

Cartoons in the era of digital media are among the most prevalent medium of entertainment for the children, parents also encourage them to view such contents to engage the children which make an area need to be explained exhaustively by adopting new approaches. There are lot of studies in the past conducted to explain the impacts of the cartoons on the children however, it is remarkable fact that there is lack of the studies in the literature which directly address the observation about the above mentioned impacts in view of the teacher’s. This study deals with the educational, emotional, behavioral and Religious etc. impacts of cartoons on kids in view of teachers of the primary schools as they are considered as the one who can observer these impacts in a profound way. of the of Multan. Different areas of impacts have been analyzed in this study by using survey technique and analysis is conducted and presented in the teacher’s views in this study to examine what they feel that how cartoons are effecting the personalities of the children. Keywords: Educational impacts, Emotional Impacts, Behavioral Impacts, Psychological Impacts, Physical Impacts, Religious Impacts

Vivax malaria and chloroquine resistance: a neglected disease as an emerging threat

In Pakistan, Plasmodium vivax contributes to major malaria burden. In this case, a pregnant woman presented with P. vivax infection and which was not cleared by chloroquine, despite adequate treatment. This is probably the first confirmed case of chloroquine-resistant vivax from Pakistan, where severe malaria due to P. vivax is already an emerging problem

Effect of Human Resource Planning on Organizational Performance of Telecom Sector

Human resource planning is one of the growing areas of academic research. Organizations regard its human resource as their core competency because it would lead towards better organizational performance. This research focuses on effect of human resource planning on the organizational performance. Objective of this research is to determine key determinants of formal human resource planning that contributes towards performance in the telecom sector. The data collected from top managers, middle level managers and the first line HR managers of 50 offices including head offices and regional offices of 160 questionnaires including some interview and open ended questions based on the measures of performance and Human Resource Planning. The results from the factor analysis on HRP measures selection, training, and incentives and the organizational performance measures which are job satisfaction, efficiency, employee motivation and technology constitutes significant and a positive relationship with other. As modern HR practices are implemented in telecom sector and companies would spend more on Human Resources, it would lead towards High performance achievement. Keywords – Human Resource Planning, Telecom Sector, Organizational Performance, Selection, Training and Incentives Paper Type­– Research Pape

Comparative bioavailability analysis of oral alendronate sodium formulations in Pakistan

Alendronate sodium, a bisphosphonate drug, it is used to treat osteoporosis and other bone diseases. The present study was designed to conduct comparative bioavailability analysis of oral formulations of alendronate sodium through an open-label, randomized, 2-sequence, 2-period crossover study. Healthy adult male Pakistani volunteers received a single 70 mg dose of the test or reference formulation of alendronate sodium followed by a 7 day washout period. Plasma drug concentrations were determined using a validated HPLC post column fluorescence derivatization method. AUC0-t, AUC0-8, Cmax and Tmax were determined by non-compartmental analysis and were found within the permitted range of 80% to 125% set by the US Food and Drug Administration (FDA). Results show that both in vitro and in vivo assays of all test brands were within the specification of the US Pharmacopoeial limits and were statistically bioequivalent. No adverse events were reported in this study

Socio – Political Context and Inferences from Remote Sensing in South Asia: A Study of Tectonic Induced Surface Deformation in SE-IKSZ

Natural or man‐made disasters are dreadful incidents that devastate lives, disturb the socioeconomic and socio-political structure of a society and preserve or erase developments and gains based on decades, within few minutes. A catastrophe has the capacity to affect existing general population to their base, parting an occasion for self-investigation and reassessment of their framework and composition. This study signifies the Radar Digital Elevation Model centered pattern of drainage network to appraise the catastrophic landslide events due to the 2005 earthquake in Neelum-Jhelum Valley in SE-Indus Kohistan Zone north of Pakistan. This investigation highlights zones affected by the earthquake and vulnerable to landslides by utilizing Hypsometric integrals (HI values) and Hack SL-gradient techniques that are proficient in detecting erosion, land mass and tectonic movements. Dataset principally includes “Shuttle Radar Topography Mission (SRTM)” Digital Elevation Model having pixel resolution of 90 meters. Hypsometric investigation brings evidence related to the deformation periods of a geographical stage. To accomplish this objective, D8 method was used, 355 subbasins of 4th Strahler order, from 5th Strahler order 75 subbasins and from 6th Strahler order 15 subbasins were delineated. To appraise the indentations of erosional scarps, Hypsometric curves (HC) and Hypsometric integrals (HI) for all distinct subbasins were computed. Variable topographic elevations (Maximun, minimum and mean) were determined to decipher the HI values. The HCs are characterized as convex up, S shaped and concave down curves. Curvature of convex up symbolizes a lesser amount of eroded or deformed subbasins (comparatively young geography), and are located in conjunction of the North-Eastern anticline side of the Muzaffarabad that indicates the tectonic behavior of HKS, however S-shaped curvatures denote the transitional stage between the convex up and concave down deformational stage. The curvatures of concave down represents extreme deformation phase and are located west and eastern side of HKS

An Efficient Malware Detection Approach for Malicious Android Application

Artificial intelligence is changing the game for cybersecurity, analyzing enormous amount of risky data, increasing response times and enlarging the abilities of under-resourced security tasks. While security as IT percentage grows at a fast pace, the cost of security beaches grows at a more rapid pace. The malware targeting Android is growing. Android systems holds more than 70 percent of the market share.This paper presents a simple APK analysis approach with the help of neural networks to identify malicious and benign application. The selected methodology is efficient in detecting malwares with an accuracy of 98.42% and false positive rate of 0.012

An acoustic investigation of primary and secondary lexical stress of Urdu

This paper investigated acoustic correlates of primary and secondary stress in Urdu language. Urdu is not a sufficiently researched language in the context of lexical stress. A few researches (Mehrotra, 1965; Hussain, 1997; Nair, 1999; Mumtaz, 2014, and Qurrat-Ul-Ain & Mahmood (2017) discussed stress in Urdu/Hindi language. Perhaps, Qurrat-Ul-Ain & Mahmood (2017) study is the first to phonetically document the presence of secondary stress in Urdu using the cue of duration. The present study focused on the four popular acoustic cues of lexical stress to see how Urdu lexical stress (primary as well as secondary) behaves against these cues. The stimuli of the study consist of six tri-syllabic words (embedded with low-back-long vowel /a:/ in all syllables) uttered by nine female Urdu speakers from Lahore. Four popular stress cues (duration, vowel quality, pitch, and intensity) have been analyzed to see their correlation with Urdu lexical stress. The analysis reveals three levels of lexical stress: primary, secondary, and unstressed. Vowel duration is the strongest cue to correlate with the levels of stress in Urdu while stressed segments prone to have higher values of intensity. Overall, a trend of lower F0 and higher formant values could be seen against stressed syllables. The study, however, needs to be expanded further by using words having other vowel sounds. Moreover, the phenomenon of word final lengthening can be taken into account in the potential researches