Galanthus krasnovii (amaryllidaceae)'nin toplam fenolik içeriği ile antioksidan kapasitenin değerlendirilmesi

Natural products have gained the great interest due to their broad spectrum of biological activities. Galanthus krasnovii was dried at shade then extracted with hexane, dichloromethane, and ethyl acetate successively. After removing of solvent by reduced pressure, crude extracts of each solvent were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation and reducing power assays were carried out for each extract. Moreover, total phenolic content was determined. Total phenolic content of hexane-, dichloromethane-, and ethyl acetate extracts were found as 60.95 mg GAE/g extract, 71.90 GAE/g extract and 58.90 GAE/g extract respectively. Dichloromethane and ethyl acetate extract revealed the moderate antioxidant activity.Doğal ürünler geniş spektrumlu biyolojik aktivite göstermelerinden dolayı yoğun ilgi görmektedirler. Galanthus krasnovii gölgede kurutulduktan sonra sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edildi. Düşük basınçta çözücü uzaklaştırıldıktan sonra her bir çözücünün ekstraktı elde edildi. Her bir ekstraktın, 1,1-difenil-2- pikralhidrazil(DPPH) radikal, 2,2'-azino-bis(3- etilbenzotiazolin-6- sülfirik asit) (ABTS) radikal katyon ve indirgeme gücü antioksidan çalışmaları gerçekleştirildi. Ayrıca, ekstraktların toplam fenolik içerikleri belirlendi. Hekzan-, diklorometan- ve etil asetat ekstraktlarının toplam fenolik içerikleri sırasıyla 60.95 mg GAE/g ekstrakt, 71.90 GAE/g ekstrakt ve 58.90 GAE/g ekstrakt olarak belirlendi. Diklorometan ve etil asetat ekstraktları orta derece aktivite gösterdi

Preparation, GIAO NMR Calculations and Acidic Properties of Some Novel 4,5-dihydro-1H-1,2,4-triazol-5-one Derivatives with Their Antioxidant Activities

Six novel 3-alkyl(aryl)-4-(p-nitrobenzoylamino)-4,5-dihydro-1H-1,2,4-triazol-5- ones (2a-f) were synthesized by the reactions of 3-alkyl(aryl)-4-amino-4,5-dihydro-1H- 1,2,4-triazol-5-ones (1a-f) with p-nitrobenzoyl chloride and characterized by elemental analyses and IR, 1H-NMR, 13C-NMR and UV spectral data. The newly synthesized compounds 2 were titrated potentiometrically with tetrabutylammonium hydroxide in four non-aqueous solvents such as acetone, isopropyl alcohol, tert-butyl alcohol and N,N-dimethylformamide, and the half-neutralization potential values and the corresponding pKa values were determined for all cases. Thus, the effects of solvents and molecular structure upon acidity were investigated. In addition, isotropic 1H and 13C nuclear magnetic shielding constants of compounds 2 were obtained by the gauge-including-atomic-orbital (GIAO) method at the B3LYP density functional level. The geometry of each compound has been optimized using the 6-311G basis set. Theoretical values were compared to the experimental data. Furthermore, these new compounds and five recently reported 3-alkyl-4-(2-furoylamino)-4,5-dihydro-1H-1,2,4-triazol-5-ones (3a–c,e,f) were screened for their antioxidant activities

Biological Property of Fritillaria imperialis L. Extract

A preliminary in vitro screening revealed the therapeutic status of extracts of Fritillaria imperialis L. that belongs to the Liliaceae family. Its tendrilled bulbs are consumed fresh or prepared in a powdered form and used as a home remedy for cough and phlegm, high fever, hemorrhage, lack of milk, treatment of abscesses, asthma, rheumatism, and eye disease. Herein, we investigated the antiproliferative, cytotoxic effects and antibacterial activities of Fritillaria imperialis L. extracts on three cancer cell lines (HeLa, HT29, and C6), and a non-cancer cells (Vero). The potential antiproliferative and cytotoxic impact of Fritillaria imperialis L. extracts were investigated in vitro through MTT and LDH measurement techniques, and its antimicrobial effects were studied with MIC and disc-zone test. The extracts of Fritillaria imperialis L. have been shown to exhibit poor antiproliferative effects and antibacterial activities on some cancer cell lines and bacteria, respectively, at even high concentration. These data suggest that Fritillaria imperialis L. extracts are low cytotoxic to cancer cell lines and Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Our results indicate that clinic consideration of Fritillaria imperialis extracts for the treatment of malignant and bacterial disease needs to be re-evaluated due to its different extraction and isolation methods

Effects of Harvest Times on the Fatty Acids Composition of Rose Hip (Rosa sp.) Seeds

This study was conducted to determine the change of fatty acids ratios of some rose hip species seeds in different harvest times. Seeds of five genotypes belonging to rose hip species (Rosa sp.) were used in the study. The fruits of species were harvested in six different ripening times and analyzed. Total oil analysis was performed for the fifth harvest only, which was determined as the optimal harvest time. As a result; total oil ratio of rose hip seeds varied as 5.22 and 6.62 g/100g respectively for accessions of Rosa dumalis (MR-12 and MR-15), 6.37 g/100g for R. canina (MR-26), 5.00 g/100g for R. dumalis ssp. boissieri (MR-46) and 5.29 g/100g for R. villosa (MR-84). Eleven fatty acids were determined in rose hip seeds. Among these fatty acids linoleic, oleic, linolenic, palmitic and stearic acids respectively had high ratio. Saturated fatty acids ratio (SFAs) was the highest in R. canina (MR-26) and the lowest in R. dumalis (MR-12); monounsaturated fatty acids ratio (MUFAs) was the highest in R. dumalis (MR-12) and the lowest in R. dumalis ssp. boissieri (MR-46); polyunsaturated fatty acids ratio (PUFAs) was the highest in R. dumalis ssp. boissieri (MR-46) and the lowest in R. dumalis (MR-12). Mono and polyunsaturated fatty acid contents obtained in this study was high; the change of fatty acid profile in the studied species in relation to harvest time was significant for some species and insignificant for others. A conclusion was reached that it is important to pay attention to qualitative and quantitative properties of seeds when conducting studies about rose hip improvement

Comparison of alpha, beta and total ODAP (beta-N-oxalyl-L-alpha,beta-diamino propionic acid) contents in winter- and spring-sown grasspea (Lathyrus sativus L.) genotypes

WOS:000285650000012There is a strong relationship between the consumption of grasspea (Lathyrus sativus L.) and "lathyrism" disease caused by a neurotoxin, beta-N-oxalyl-L-a,beta-diaminopropionoc acid called ODAP or BOAA. The objective of this study was to compare alpha, beta and total ODAP found in grasspea genotypes sown in winter and spring seasons during 2007/08 and grown under rainy conditions in semi-arid regions of Turkey. Biochemical compounds of alpha, beta and total ODAP were found to be higher in spring-sown grass peas than those of winter-sown ones. Grasspea 452, 508 and 519 genotypes had lower beta-ODAP levels in winter-and spring-sown

Effects of leaf removals on must and wine chemical composition and phenolic compounds of Narince (Vitis vinifera) grape cultivar

Leaves are the vital components of grapevines. Leaves are removed with summer pruning to improve grape and wine quality. Leaves of some grape cultivars are harvested at different periods for stuffed grape leaves or brined vine leaves. Since the leaves of Narince grape cultivar supplies an income as much as the grapes, the cultivars are commonly exposed to excessive leaf harvests. The present study was conducted in 2014–2015 to investigate the effects of 4 different leaf harvest treatments (LHT) (control, 2, 4 and 6 harvests) on must and wine quality of Narince grape cultivar grown in Tokat province of Turkey. The grapes harvested at technological ripening level were processed into wine with microvinification method. The pH, total soluble solid contents (TSSC), titratable acidity, specific gravity, ethyl alcohol, volatile acid, reducing sugar, total sulphur dioxide, total phenolics, total flavonoids and some phenolic compounds of the must and wines were determined. LHTs increased TSSC and total phenolics of the must; pH, ethyl alcohol, total phenolics and total flavonoids of the wines increased in six LHT as compared to control treatment. While LHTs increased phenolic compounds of catechin, epicatechin and caffeic acid as compared to control treatment, coumaric acid was not observed in wines. Wine sensory scores were the highest in control treatment and the lowest in six leaf harvest treatments

Changes of phenolic compounds in tomato associated with the heavy metal stress

Heavy metals have restricted the plant regular life cycles affecting the plant primer and seconder metabolites by biochemical and physiological pathways. Phenolic compounds considered as products of metabolic alterations have been synthesized in various numbers and typical characteristic of plants. In this study, we aimed to investigate the variations of phenolic compounds with HPLC in leaves of tomato exposed to heavy metals. The applications of Cu, Cd and Pb significantly reduced the total phenolic content, levels of caffeic, chlorogenic and vanillic acids in all treated groups except for 50 and 20 ppm of Pb for total phenolics and vanillic acid, respectively. The level of benzoic acid is generally decreased by the application of heavy metals except for Cu at 50 ppm doses. Rutin is the most abundant phenolic compound in term of quantity among to analyzed phenolics and its content decreased depending on the heavy metal doses except for 10 ppm doses of Cd. The responses of tomato under heavy metals stress resulted in lower amount of phenolic compounds. In the present study, it was showed that total phenolic content has positive correlations with caffeic acid in all treatment of heavy metals