Biochemical, Hematological and Histopathological Alterations in Liver and Lung of Rats Due to Sub-chronic Inhalation Exposure to NPK 15.15.15 Fertilizer

The current study has been conducted to evaluate biochemical, hematological and histopathological alterations in rats following one month of inhalation exposure to NPK (nitrate, phosphate, potassium) 15.15.15 fertilizer. 24 male Wistar rats were randomly divided into four groups (A, B, C and D). Group A was served as control, whereas B, C and D groups have been exposed to aerosol of different doses (10 mg/m3, 30 mg/m3 and 50 mg/m3, respectively) of NPK 15.15.15 fertilizer during 30 min/day for one month. At the end of the experiment, rats were sacrificed. Blood samples were collected to evaluate biochemical and hematological parameters. Liver and lung tissues were served to evaluate oxidative stress statue and histopathological alterations. Our results showed that inhalation exposure of rats to NPK 15.15.15 fertilizers, especially at high dose (50 mg/m3) resulted in a significant (P = 0,05) elevation of biochemical hepatic markers: Aspartate aminotransferase and Alanine transaminase, and a highly significant (P = 0,01) elevation of alkaline phosphatase. In addition, there were significant changes in hematological parameters revealed mainly by a highly significant (P = 0,01) increase of lymphocytes in both groups C and D groups, and significant decreases (P = 0,05) in all of: red blood cells, hematocrit, and hemoglobin in rats of group D compared to control. Furthermore, the inhalation exposure of rats to NPK 15.15.15 fertilizers induced lung oxidative stress revealed by the reduction of anti-oxidant enzymes (Glutathione peroxidase, reduced glutathione and superoxide dismutase); besides we have observed significant histopathological alterations revealed mainly by inflammatory cell infiltration in liver and lung tissues. The inhalation exposure to important doses of NPK 15.15.15 fertilizer can damage the liver and lung tissues by altering biochemical, hematological and oxidative stress parameters

Chemical Composition, Antioxidant and Antimicrobial Activities of the Aqueous Extract of Syzygium aromaticum (Clove) from Indonesia

The clove (Syzygium aromaticum) is an aromatic herb that originates mainly from Central Asia and Africa. This herb has been used in many ways, especially in the medical field. Given its many therapeutic benefits, it would be interesting to identify the different phytochemicals of this plant. The primary objective of this work is to measure the various phenolic compounds in the aqueous extract of the plant and to identify them, using the HPLC technique. The study of the antioxidant activity in vitro of this extract as well as its antimicrobial activity have been carefully carried out. According to the results of the phytochemical analysis, this plant contained, more or less, significant amounts of phenolic compounds such as total phenols (176.02 mg), flavonoids (23.5 mg) and ortho-diphenols (740,14 mg). Moreover, some phenolic compounds, in particular flavonols, were present in smaller amounts, (2.09 x 10-3 mg). The presence of certain compounds was then confirmed by the HPLC technique. The results of the study of the antioxidant activity of clove extract (DPPH, ABTS and β-carotene test), showed values that ​​were high. As for the antibacterial activity tests, they showed remarkably high inhibition zones and sensitivity (diameter 18mm and 15mm diameter) for the Gram negative bacteria : Pseudomonas aeruginosa and Escherichia Coli. As regards for the gram-positive strain which is Staphylococcus aureus, it had a high sensitivity and an inhibition diameter of (19 mm)

Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model

Context: To find bioactive medicinal herbs exerting anti-asthmatic activity, we investigated the effect of an aqueous extract of Urtica dioica L. (Urticaceae) leaves (UD), the closest extract to the Algerian traditional use. Objective: In this study, we investigated the in vivo anti-asthmatic and antioxidant activities of nettle extract. Materials and methods: Adult male Wistar rats were divided into four groups: Group I: negative control; group II: Ovalbumin sensitized/challenged rats (positive control); group III: received UD extract (1.5 g/kg/day) orally along the experimental protocol; group IV: received UD extract (1.5 g/kg/day) orally along the experimental protocol and sensitized/challenged with ovalbumin. After 25 days, blood and tissue samples were collected for haematological and histopathological analysis, respectively. The oxidative stress parameters were evaluated in the lungs, liver and erythrocytes. Then, correlations between markers of airway inflammation and markers of oxidative stress were explored. Results: UD extract significantly (p < 0.01) inhibited eosinophilia increases in BALF (−60%) and the levels of leucocytes (−32.75%) and lymphocytes (−29.22%) in serum, and effectively suppressed inflammatory cells recruitment in the asthmatic rat model. Besides, the lipid peroxidation generated by allergen administration was significantly (p < 0.05) diminished by UD treatment in lung tissue (−48.58%). The nettle extract was also investigated for the total phenolic content (30.79 ± 0.96 mg gallic acid/g dry extract) and shows DPPH radical scavenging activity with 152.34 ± 0.37 μg/mL IC50 value. Conclusions: The results confirmed that UD administration might be responsible for the protective effects of this extract against airway inflammation

AMELIORATIVE EFFECTS OF VANILLIN AGAINST METRIBUZIN-INDUCED OXIDATIVE STRESS AND TOXICITY IN RATS

Objective: Vanillin (Va), a phenolic substance is a natural antioxidant with proven free radical scavenging activity and offers protection against oxidative damage. This study was designed to investigate the ameliorative effect of Va against metribuzin (Mtz) pesticide-induced toxicity and oxidative stress in rats.Methods: Thirty-two rats were equally divided into four groups: control, vanillin (Va), metribuzin (Mtz) and metribuzin plus vanillin (Mtz+Va). Metribuzin (133.33 mg/kg, 1/20 LD50) was given to rats through their drinking water. Vanillin was supplemented (150 mg/kg) intraperitoneally for three weeks. At the end of experiments, various haematological and biochemical parameters were estimated. Antioxidant defence systems as GPx enzyme activities, GSH and MDA contents were determined in tissues as the indicators of oxidative stress.Results: Compared with the control, a significant decrease in the levels of red blood cells (p≤0.05), haemoglobin (p≤0.003) and hematocrit (p≤0.02) were noticed in the metribuzin-exposed group. The biochemical evaluation showed significantly higher values (p≤0.0001) for glucose, urea, creatinine and bilirubin. Also, Mtz treatment caused significant perturbations in lipids profile as plasma levels of total cholesterol (p≤0.0001) and triglycerides (p≤0.004). MDA levels increased in all the tissues of rats exposed to Mtz whereas the fluctuation in GPX enzyme activities and GSH levels were observed compared with controls. However, treatment with vanillin significantly normalised haematological profiles, serum biochemical profile and modulated all the oxidative stress parameters.Conclusion: The use of vanillin appeared to be beneficial to rats, to a great extent in attenuating and restoring the damage sustained by metribuzin exposure