Endophytic Mycoflora of Indian Medicinal Plant, Terminalia arjuna and their Biological Activities

Studies were undertaken to isolate, identify and evaluate the biological activities of endophytic mycoflora of Indian medicinal plant, Terminalia arjuna. A total of 20 isolates of endophytic fungi were obtained from the leaves, twigs and bark tissues of the Terminalia arjuna. Out of 20, six isolates exhibited promising antibacterial, antifungal and anti-inflammatory activities when cultivated at shake flask level. The selected isolates were identified on the basis of morphology and ITS gene sequencing. Three isolates, designated as TA BA 1, TA L1 and TA L2 were identified as Aspergillus flavus whereas; the remaining three endophytic fungi were identified as Diaporthe arengae (TA TW2), Alternaria Sp. (TA TW1) and Lasiodiplodia theobromae (TA BA2). Aspergillus flavus was found as the predominant endophyte in leaves and bark tissues of the plant. The crude extract of the test isolates showed considerable antimicrobial activity against common human bacterial (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella abony and Bacillus subtilis) and fungal (Candida albicans, Aspergillus niger and Penicilium sp.) pathogens. The extract of Diaporthe arengae (TA TW2) significantly reduced the concentration of DPPH free radical as percent DPPH scavenging activity was found to be highest (69.56%) in comparison with other isolates. The % inhibition of hemolysis of RBCs was found to be highest (82.85%) with Diaporthe arengae (TA TW2) in comparison (83.26%) with standard drug (Ibuprofen). Among all, the extract of the Diaporthe arengae (TA TW2) showed excellent biological activities and hence was subjected to further characterization. The phytochemical investigation of the extract revealed the presence of terpenoids as the major phytoconstituents which was supported by TLC and UV spectroscopic studies. The results indicate that the isolated endophytes could be the valuable source of these bioactive molecules with diverse biological activities. The bioactivities may be attributed to the terpenoids present in the endophytic extract

The impact of irrigation with treated wastewaters on soil and kikuyu grass nutrient compositions

A lysimeter study was conducted for 1 year to examine how the source of wastewater for irrigation impacted soil physicochemical properties and kikuyu grass (Pennisetum clandestinum) nutrient composition. The wastewater used included treated wastewater produced by a membrane bioreactor (MBR) and intermittently decanted aerated lagoon (IDAL) treatment systems. No significant differences were observed between the treatments regarding total nitrogen and total phosphorus across the depths of the columns. However, highly significant differences were observed for Na content of the soils at various depths. Remarkable differences were recorded for soil exchangeable K and Na at different depths. In contrast, soil exchangeable Ca and Mg experienced no significant differences concerning the depth of the columns. For kikuyu grass, sodium contents of the grasses irrigated with MBR and IDAL treated wastewaters increased more than 200% and 100%, respectively, when compared with the grass irrigated with tap water. Over the period of monitoring considered in this study, there was no sign of excessive soil salinity/sodicity issues. The MBR treated wastewater has the potential to supply the grass with a constant dosage of valuable nutrients such as N and P without the requirement of using chemical fertilizers. This reduces the risk of contamination of receiving waters and groundwater and enhances the recycling of the nutrients in the wastewater to achieve a circular economy of nutrients

Scaling law in target-hunting processes

We study the hunting process for a target, in which the hunter tracks the goal by smelling odors it emits. The odor intensity is supposed to decrease with the distance it diffuses. The Monte Carlo experiment is carried out on a 2-dimensional square lattice. Having no idea of the location of the target, the hunter determines its moves only by random attempts in each direction. By sorting the searching time in each simulation and introducing a variable $x$ to reflect the sequence of searching time, we obtain a curve with a wide plateau, indicating a most probable time of successfully finding out the target. The simulations reveal a scaling law for the searching time versus the distance to the position of the target. The scaling exponent depends on the sensitivity of the hunter. Our model may be a prototype in studying such the searching processes as various foods-foraging behavior of the wild animals.Comment: 7 figure

Impact of recycled water irrigation on soil salinity and its remediation

Continuous use of recycled water (treated wastewater) over a long period of time may lead to the accumulation of salt in the root zone of the soil. This is due to the relatively higher levels of salt contained in the recycled water compared to a town water supply. The increase in salt concentration in the soil can adversely influence the amount of water a plant can uptake from the soil due to the osmotic effect. Despite significant benefits, recycled water may deteriorate soil health in terms of increased salinity and sodicity. Although several studies in the past have highlighted the increase of soil salinity due to recycled water irrigation, the phenomenon depends on the variability of soil characteristics. In this study, the impact of using three different types of irrigation water (with electrical conductivity 0.2, 0.8, and 2.0 dS/m) on the mechanism of salt accumulation in the soil was investigated. To contribute to the addition of the existing knowledge of soil salinisation, soil from two paddocks (i.e., D33 and Yarramundi) in Western Sydney, Australia were analysed, and relationships among parameters associated with salt accumulation were evaluated using the results from continuous column studies. Results show that if the irrigation is conducted with high saline water, there is a possibility for salinisation of soil to occur. To prevent this from occurring, one of the solutions could be to use a normal town water supply for irrigation at some intervals. This will allow for the leaching of excess salt accumulated in the soil to deeper layers

Quantum mechanics in multiply connected spaces

This paper analyses quantum mechanics in multiply connected spaces. It is shown that the multiple connectedness of the configuration space of a physical system can determine the quantum nature of physical observables, such as the angular momentum. In particular, quantum mechanics in compactified Kaluza-Klein spaces is examined. These compactified spaces give rise to an additional angular momentum which can adopt half-integer values and, therefore, may be identified with the intrinsic spin of a quantum particle.Comment: Latex 15 page

Molecular analysis of autosomal dominant hereditary ataxias in the Indian population: high frequency of SCA2 and evidence for a common founder mutation

Expansion of CTG/CAG trinucleotide repeats has been shown to cause a number of autosomal dominant cerebellar ataxias (ADCA) such as SCA1, SCA2, SCA3/MJD, SCA6, SCA7, SCA8 and DRPLA. There is a wide variation in the clinical phenotype and prevalence of these ataxias in different populations. An analysis of ataxias in 42 Indian families indicates that SCA2 is the most frequent amongst all the ADCAs we have studied. In the SCA2 families, together with an intergenerational increase in repeat size, a horizontal increase with the birth order of the offspring was also observed, indicating an important role for parental age in repeat instability. This was strengthened by the detection of a pair of dizygotic twins with expanded alleles showing the same repeat number. Haplotype analysis indicates the presence of a common founder chromosome for the expanded allele in the Indian population. Polymorphism of CAG repeats in 135 normal individuals at the SCA loci studied showed similarity to the Caucasian population but was significantly different from the Japanese population

Effective one-body approach to the relativistic two-body problem

The relativistic 2-body problem, much like the non-relativistic one, is reduced to describing the motion of an effective particle in an external field. The concept of a relativistic reduced mass and effective particle energy introduced some 30 years ago to compute relativistic corrections to the Balmer formula in quantum electrodynamics, is shown to work equally well for classical electromagnetic and gravitational interaction. The results for the gravitational 2-body problem have more than academic interest since they apply to the study of binary pulsars that provide precision tests for general relativity. They are compared with recent results derived by other methods.Comment: 9 pages, latex, no figures. Minor amendments, comments, new references and acknowledgments adde

Consanguinity Mapping of Congenital Heart Disease in a South Indian Population

Parental consanguinity is a risk factor for congenital heart disease (CHD) worldwide, suggesting that a recessive inheritance model may contribute substantially to CHD. In Bangalore, India, uncle-niece and first cousin marriages are common, presenting the opportunity for an international study involving consanguinity mapping of structural CHD. We sought to explore the recessive model of CHD by conducting a genome-wide linkage analysis utilizing high-density oligonucleotide microarrays and enrolling 83 CHD probands born to unaffected consanguineous parents. exons did not reveal causative mutations in Indian probands. In addition, genotyping of the linked allele (G) in 325 U.S. CHD cases revealed neither genotypic nor allele frequency differences in varied CHD cases compared to 605 non-CHD controls. may harbor recessive mutations leading to CHD in the Indian population. Further research involving large multinational cohorts of patients with specific subtypes of CHD is needed to attempt replication of the observed linkage peak on chromosome 14. In addition, we anticipate that a targeted re-sequencing approach may complement linkage analysis in future studies of recessive mutation detection in CHD

Coalescence of Two Spinning Black Holes: An Effective One-Body Approach

We generalize to the case of spinning black holes a recently introduced ``effective one-body'' approach to the general relativistic dynamics of binary systems. The combination of the effective one-body approach, and of a Pad\'e definition of some crucial effective radial functions, is shown to define a dynamics with much improved post-Newtonian convergence properties, even for black hole separations of the order of $6 GM / c^2$. We discuss the approximate existence of a two-parameter family of ``spherical orbits'' (with constant radius), and, of a corresponding one-parameter family of ``last stable spherical orbits'' (LSSO). These orbits are of special interest for forthcoming LIGO/VIRGO/GEO gravitational wave observations. It is argued that for most (but not all) of the parameter space of two spinning holes the effective one-body approach gives a reliable analytical tool for describing the dynamics of the last orbits before coalescence. This tool predicts, in a quantitative way, how certain spin orientations increase the binding energy of the LSSO. This leads to a detection bias, in LIGO/VIRGO/GEO observations, favouring spinning black hole systems, and makes it urgent to complete the conservative effective one-body dynamics given here by adding (resummed) radiation reaction effects, and by constructing gravitational waveform templates that include spin effects. Finally, our approach predicts that the spin of the final hole formed by the coalescence of two arbitrarily spinning holes never approaches extremality.Comment: 26 pages, two eps figures, accepted in Phys. Rev. D, minor updating of the text, clarifications added and inclusion of a few new reference