Anatomic variation of celiac axis and hepatic artery as evidenced multidetector computed tomography in patients at tertiary care center in Nepal

Introduction: There are many potential anatomic configurations of celiac axis and hepatic artery. Michel’s classified hepatic arterial variations in 10 categories. Knowledge about these variantion in patient is invaluable before surgery. Multidetector computed tomography (MDCT) can accurately depict the vascular anatomy of celiac axis and hepatic artery non-invasively. Aim of this study was to find out the prevalence of different types of anatomical variation of celiac axis and hepatic artery in patients undergoing multiphase CT. Method: Cross-sectional, prospective study was done in Department of Radiology, Patan Hospital, Patan Academy health sciences. MDCT of abdomen of 178 patients with arterial phase images done between December 2021 and March 2022 were evaluated for arterial anatomy of celiac axis and hepatic artery. Arterial anatomy was categorized according to Michel’s classification. Prevalence of each variant anatomy and gender wise prevalence was calculated. Results: CT scans of 178 patients were evaluated out of which 94 were male and 84 were female. Among these individuals 133 (74.7%) had normal anatomy (Type I) and 45 (25.3%) had some sort of variant anatomy. Type III was commonest type of variant anatomy seen in 18 (10.1%) individuals. We found 6(3.4%) individuals with the anatomy other than classified by Michel. Conclusion: There are multiple variation in of celiac axis and hepatic artery anatomy in large number of populations. Knowledge of such anatomical variation will be of great importance for surgeon and interventional radiologist for planning of surgical or vascular procedures and to prevent vascular complication

A foreign body response-on-a-chip platform

Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR?on?a?chip (FBROC) for modeling the cascade of events during immune cell response to implants. The platform models the native implant microenvironment where the implants are interfaced directly with surrounding tissues, as well as vasculature with circulating immune cells. The study demonstrates that the release of cytokines such as monocyte chemoattractant protein 1 (MCP?1) from the extracellular matrix (ECM)?like hydrogels in the bottom tissue chamber induces trans?endothelial migration of circulating monocytes in the vascular channel toward the hydrogels, thus mimicking implant?induced inflammation. Data using patient?derived peripheral blood mononuclear cells further reveal inter?patient differences in FBR, highlighting the potential of this platform for monitoring FBR in a personalized manner. The prototype FBROC platform provides an enabling strategy to interrogate FBR on various implants, including biomaterials and engineered tissue constructs, in a physiologically relevant and individual?specific manner

Reversed-engineered human alveolar lung-on-a-chip model

Here, we present a physiologically relevant model of the human pulmonary alveoli. This alveolar lung-on-a-chip platform is composed of a three-dimensional porous hydrogel made of gelatin methacryloyl with an inverse opal structure, bonded to a compartmentalized polydimethylsiloxane chip. The inverse opal hydrogel structure features well-defined, interconnected pores with high similarity to human alveolar sacs. By populating the sacs with primary human alveolar epithelial cells, functional epithelial monolayers are readily formed. Cyclic strain is integrated into the device to allow biomimetic breathing events of the alveolar lung, which, in addition, makes it possible to investigate pathological effects such as those incurred by cigarette smoking and severe acute respiratory syndrome coronavirus 2 pseudoviral infection. Our study demonstrates a unique method for reconstitution of the functional human pulmonary alveoli in vitro, which is anticipated to pave the way for investigating relevant physiological and pathological events in the human distal lung

Electric vehicle technology in Kathmandu, Nepal : a closer look at development

Thesis (M.C.P.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 2002.Includes bibliographical references (leaves 86-92).Electric vehicle (EV) development in the Kathmandu Valley began in 1993 as a response to the urgency of a severe air pollution situation. The dynamics of government intervention, non-governmental organization advocacy, international donor support, and private sector involvement all shaped EV implementation in various ways. Its success led other South and East Asian cities to view it as a model for implementing EVs to alleviate air pollution. Yet despite a promising beginning and intensive proliferation, the EV industry was failing only six years after its inception. What went wrong with a development that seemed to have all the makings of success? This thesis outlines the EV development trajectory and examines the principal factors that impeded progress. Interviews with over 30 individuals in the electric vehicle industry, government agencies, NGOs, and international donor organizations provided me with first-hand accounts of the puzzles of EV development. Also, my research in published and unpublished documents, local press coverage, and an EV news server added rich material for analysis. The most entrenched barriers to the implementation of the EV industry have been the disparate interests and goals of stakeholders, in particular the resistance and hostility of fossil-fuel interests, and deficiencies in human resources and support networks. Analysis of these impediments yields lessons on how EV advocates can overcome these obstacles. Lessons learned in this thesis are that EV advocates must build a coalition of supportive actors, seek governmental commitment for EV-supportive polices, work to align the disparate economic goals of private actors, and develop a capacity for training and education.by Sushila Maharjan.M.C.P

A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing

Modular microfluidics offer the opportunity to combine the precise fluid control, rapid sample processing, low sample and reagent volumes, and relatively lower cost of conventional microfluidics with the flexible reconfigurability needed to accommodate the requirements of target applications such as drug toxicity studies. However, combining the capabilities of fully adaptable modular microelectromechanical systems (MEMS) assembly with the simplicity of conventional microfluidic fabrication remains a challenge. A hybrid polydimethylsiloxane (PDMS)-molding/photolithographic process is demonstrated to rapidly fabricate LEGO®-like modular blocks. The blocks are created with different sizes that interlock via tongue-and-groove joints in the plane and stack via interference fits out of the plane. These miniature strong but reversible connections have a measured resistance to in-plane and out-of-plane forces of up to >6000× and >1000× the weight of the block itself, respectively. The LEGO®-like interference fits enable O-ring-free microfluidic connections that withstand internal fluid pressures of >120 kPa. A single layer of blocks is assembled into LEGO®-like cell culture plates, where the in vitro biocompatibility and drug toxicity to lung epithelial adenocarcinoma cells and hepatocellular carcinoma cells cultured in the modular microwells are measured. A double-layer block structure is then assembled so that a microchannel formed at the interface between layers connects two microwells. Breast tumor cells and hepatocytes cultured in the coupled wells demonstrate interwell migration as well as the simultaneous effects of a single drug on the two cell types

Multiscale bioprinting of vascularized models

A basic prerequisite for the survival and function of three-dimensional (3D) engineered tissue constructs is the establishment of blood vessels. 3D bioprinting of vascular networks with hierarchical structures that resemble in vivo structures has allowed blood circulation within thick tissue constructs to accelerate vascularization and enhance tissue regeneration. Successful rapid vascularization of tissue constructs requires synergy between fabrication of perfusable channels and functional bioinks that induce angiogenesis and capillary formation within constructs. Combinations of 3D bioprinting techniques and four-dimensional (4D) printing concepts through patterning proangiogenic factors may offer novel solutions for implantation of thick constructs. In this review, we cover current bioprinting techniques for vascularized tissue constructs with vasculatures ranging from capillaries to large blood vessels and discuss how to implement these approaches for patterning proangiogenic factors to maintain long-term, stimuli-controlled formation of new capillaries

Needle-Free Immunization with Chitosan-Based Systems

Despite successful use, needle-based immunizations have several issues such as the risk of injuries and infections from the reuse of needles and syringes and the low patient compliance due to pain and fear of needles during immunization. In contrast, needle-free immunizations have several advantages including ease of administration, high level of patient compliance and the possibility of mass vaccination. Thus, there is an increasing interest on developing effective needle-free immunizations via cutaneous and mucosal approaches. Here, we discuss several methods of needle-free immunizations and provide insights into promising use of chitosan systems for successful immunization

Galactosylated Poly(Ethyleneglycol)-Lithocholic Acid Selectively Kills Hepatoma Cells, While Sparing Normal Liver Cells

Delivering drugs selectively to cancer cells but not to nearby normal cells is a major obstacle in drug therapy. In this study, lithocholic acid (LCA), a potent anti-cancer drug, is converted to two forms of poly(ethyleneglycol) (PEG) conjugates, viz., PEG-LCA (PL) and lactobionic acid (LBA) conjugated PEG-LCA (LPL). The latter form contains a galactose ligand in LBA to target the hepatocytes. Both forms are self-assembled to form nanoparticle formulation, and they have high potency than LCA to kill HepG2 cancer cells, sparing normal LO2 cells. Besides, LPL has high specificity to mouse liver cells in vivo. Western blot results confirm that the cell death is occurred through apoptosis induced by LPL nanoparticles. In conclusion, the induction of apoptosis and cell death is much more efficient with LPL nanoparticles than LCA molecules

Forests of learning : experiences from research on an adaptive collaborative approach to community forestry in Nepal; a synthesis of lessons from the Adaptive Collaborative Management Research Project in Nepal, 1999–2002 and 2004–2007

In recent years, awareness has grown in Nepal and globally regarding two of community forestry’s most critical challenges: equity and livelihoods. Yet even as understanding of these challenges has improved, actors from the local to the national levels in Nepal continue to be confronted with the dilemma of how to address these challenges in such a diverse, complex and dynamic context. This synthesis explores an adaptive collaborative approach to governance and management as one avenue to meet these challenges. This approach integrates inclusive decision making, networking, social learning, and pro active adjustments of practice and policies based on learning. The synthesis’ lessons are drawn from a six-year partnership-based research initiative in Nepal—spearheaded by the Center for International Forestry Research—which spanned the local, district and national levels. Key points of learning discussed in this book include factors, processes and arrangements that support—or limit—adaptive and collaborative capacities, such as active facilitation, ‘nested’ decision making, and learning-based monitoring. The book also explores both the conceptual underpinnings of the approach as well as its effects in research sites, including in terms of benefits for the poor, women and other traditionally marginalised people. This book is intended as a resource for policy makers and civil society practitioners alike, as well as researchers and others interested in pro-equity and livelihood innovations in community forestry. Through its clear conceptual and research lesson focus, this synthesis complements and is a sister publication to the hands-on guidebook entitled Facilitating Forests of Learning