Detection of early osteogenic commitment in primary cells using Raman spectroscopy

Major challenges in the development of novel implant surfaces for artificial joints include osteoblast heterogeneity and the lack of a simple and sensitive in vitro assay to measure early osteogenic responses. Raman spectroscopy is a label-free, non-invasive and non-destructive vibrational fingerprinting optical technique that is increasingly being applied to detect biochemical changes in cells. In this study Raman spectroscopy has been used to obtain bone cell-specific spectral signatures and to identify any changes therein during osteoblast commitment and differentiation of primary cells in culture. Murine calvarial osteoblasts (COBs) were extracted and cultured and studied by Raman spectroscopy over a 14 day culture period. Distinct osteogenic Raman spectra were identified after 3 days of culture with strong bands detected for mineral: phosphate ν3 (1030 cm−1) and B-type carbonate (1072 cm−1), DNA (782 cm−1) and collagen matrix (CH2 deformation at 1450 cm−1) and weaker phosphate bands (948 and 970 cm−1). Early changes were detected by Raman spectroscopy compared to a standard enzymatic alkaline phosphatase (ALP) assay and gene expression analyses over this period. Proliferation of COBs was confirmed by fluorescence intensity measurements using the Picogreen dsDNA reagent. Changes in ALP levels were evident only after 14 days of culture and mRNA expression levels for ALP, Col1a1 and Sclerostin remained constant during the culture period. Sirius red staining for collagen deposition also revealed little change until day 14. In contrast Raman spectroscopy revealed the presence of amorphous calcium phosphate (945–952 cm−1) and carbonated apatite (957–962 cm−1) after only 3 days in culture and octacalcium phosphate (970 cm−1) considered a transient mineral phase, was detected after 5 days of COBs culture. PCA analysis confirmed clear separation between time-points. This study highlights the potential of Raman spectroscopy to be utilised for the early and specific detection of proliferation and differentiation changes in primary cultures of bone cells

Two-fluid temperature-dependent relativistic waves in magnetized streaming pair plasmas

A relativistic two-fluid temperature-dependent approach for a streaming magnetized pair plasma is considered. Such a scenario corresponds to secondary plasmas created at the polar caps of pulsar magnetospheres. In the model the generalized vorticity rather than the magnetic field is frozen into the fluid. For parallel propagation four transverse modes are found. Two are electromagnetic plasma modes which at high temperature become light waves. The remaining two are Alfveacutenic modes split into a fast and slow mode. The slow mode is cyclotron two-stream unstable at large wavelengths and is always subluminous. We find that the instability cannot be suppressed by temperature effects in the limit of large (finite) magnetic field. The fast Alfveacuten mode can be superluminous only at large wavelengths, however it is always subluminous at high temperatures. In this incompressible approximation only the ordinary mode is present for perpendicular propagation. For oblique propagation the dispersion relation is studied for finite and large strong magnetic fields and the results are qualitatively described.Institute for Fusion Studie

On a Raychaudhuri equation for hot gravitating fluids

We generalize the Raychaudhuri equation for the evolution of a self gravitating fluid to include an Abelian and non-Abelian hybrid magneto fluid at a finite temperature. The aim is to utilize this equation for investigating the dynamics of astrophysical high temperature Abelian and non-Abelian plasmas.Comment: 13 pages, Invited contribution to Pramana special issue dedicated to A.K. Raychaudhuri, "The Raychaudhuri equation and its role in Modern Cosmology". Pramana style files include

HIV/AIDS, Security and Conflict: New Realities, New Responses

Ten years after the HIV/AIDS epidemic itself was identified as a threat to international peace and security, findings from the three-year AIDS, Security and Conflict Initiative (ASCI)(1) present evidence of the mutually reinforcing dynamics linking HIV/AIDS, conflict and security

Electronic structure of spin 1/2 Heisenberg antiferromagnetic systems: Ba_2Cu(PO_4)_2 and Sr_2Cu(PO_4)_2

We have employed first principles calculations to study the electronic structure and magnetic properties of the low-dimensional phosphates, Ba2Cu(PO4)2 and Sr2Cu(PO4)2. Using the self-consistent tight-binding lin- earized muffin-tin orbital method and the Nth order muffin-tin orbital method, we have calculated the various intrachain as well as the interchain hopping parameters between the magnetic ions Cu2+ for both the com- pounds. We find that the nearest-neighbor intrachain hopping t is the dominant interaction suggesting the compounds to be indeed one dimensional. Our analysis of the band dispersion, orbital projected band struc- tures, and the hopping parameters confirms that the Cu2+-Cu2+ super-super exchange interaction takes place along the crystallographic b direction mediated by O-P-O. We have also analyzed in detail the origin of short-range exchange interaction for these systems. Our ab initio estimate of the ratio of the exchange inter- action of Sr2Cu(PO4)2 to that of Ba2Cu(PO4)2 compares excellently with available experimental results.Comment: 6 pages, 4 figure

Microscopic origin of local moments in a zinc-doped high-TcT_{c} superconductor

The formation of a local moment around a zinc impurity in the high-$T_{c}$ cuprate superconductors is studied within the framework of the bosonic resonating-valence-bond (RVB) description of the $t-J$ model. A topological origin of the local moment has been shown based on the phase string effect in the bosonic RVB theory. It is found that such an $S=1/2$ moment distributes near the zinc in a form of staggered magnetic moments at the copper sites. The corresponding magnetic properties, including NMR spin relaxation rate, uniform spin susceptibility, and dynamic spin susceptibility, etc., calculated based on the theory, are consistent with the experimental measurements. Our work suggests that the zinc substitution in the cuprates provide an important experimental evidence for the RVB nature of local physics in the original (zinc free) state.Comment: The topological reason of local moment formation is given. One figure is adde

The Relativistic Generalization of the Gravitational Force for Arbitrary Spacetimes

It has been suggested that re-expressing relativity in terms of forces could provide fresh insights. The formalism developed for this purpose only applied to static, or conformally static, space-times. Here we extend it to arbitrary space-times. It is hoped that this formalism may lead to a workable definition of mass and energy in relativity.Comment: 16 page

Active utilization of a full DC-Link voltage in multilevel converter

© 2018 IEEE. Multilevel inverter technology has emerged recently as a very important alternative in the area of high-power medium-voltage energy conversion. Multilevel inverter reduces the inductors and filters size, whilst improving the output power quality. However, the main drawback of the multi-level inverter topologies is that they utilizes only ≤ 50% of the input dc-bus voltage, i.e. they require two times the peak of ac output voltage. For example, the nominal input voltage of the NPC, ANPC and Flying Capacitor is 800 V dc . This high dc-link voltage not only requires higher voltage components (both active and passive) but also prompts to use an additional front-end boost dc-dc converter. Considering these aspects, this paper presents a novel technique to extend the input dc-bus voltage utilization in any conventional multilevel inverter from ≤ 50% to ≤ 100%. The novel technique utilizes an additional T-type module (consist of four active switches), which is inserted just before the two dc-link capacitor forming a new grounding point. The novel method not only reduces the input voltage requirement and voltage stress, but also increases the output voltage levels of the inverter. In general, this technique can be implemented to any multilevel inverter. An example of implementation of 5L inverter from the conventional 3-Level T-type inverter is discussed and validated. Measurement results shows that the new Dual T-type inverter has a flat efficiency « 99 % over a wide range of load