Fecundity results from the different genotypes of the F₁₀ intercross strain.

<p>A) total egg count during the 2 week collecting period and B) total egg mass as a percent of body mass. A⁺/A⁺ is wild type, A⁺/A^D is heterozygote and A^D/A^D is domestic genotype. The horizontal line shows the mean value and the dots are the individual data points.</p

ADRA2C abundance from receptor binding assays.

<p>A) Receptor abundance in 3 brain regions with all animals pooled. Abundance in amygdala (B), hypothalamus (C) and striatum (D). The line indicate the mean value and dots indicate individual data points.</p

Positional information on the location of ADRA2C in relation to a neighboring selective sweep and methylated regions from previous studies.

<p>A) Location of the <i>ADRA2C</i> in chromosome 4 in relation to the selective sweep region reported by Rubin et al. (2010). All coordinates are expressed as kb. The position of the 1229 bp long gene starting at 84590769 is represented by a blue box and the SNP in position 84590997 used for genotyping is shown as a yellow star within the gene. The direction of transcription is indicated with an arrow. The methylation pattern bp long gene starting at 84590769 is represented by a blue box and the SNP in position 84590997 used for genotyping is shown as a yellow star within the gene. The direction of transcription is indicated with an arrow. The methylation pattern (in Log2 fold change) comparing RJF and WL, shown with a resolution of 50–75 bp regions is shown in the bar graph, and the hypermethylated region is highlighted with a red bar at position 84593087. Methylation data from N bp regions is shown in the bar graph, and the hypermethylated region is highlighted with a red bar at position 84593087. Methylation data from Nätt et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103218#pone.0103218-Natt1" target="_blank">[16]</a>. B) Peptide sequence homology phylogeny of the annotated sequences of α₂ adrenergic receptor subtypes cluster the chicken <i>ADRA2C</i> gene to better characterized mammalian ADRA2C genes.</p

Adrenergic receptor gene expression in adrenal glands.

<p>A) <i>ADRA2C</i> and B) <i>ADRA2A</i> where the line indicates average value relative to housekeeping genes and the dots are the individual data points.</p

Behavioural variables from Tonic Immobility (TI) and Aerial Predator tests.

<p>A) TI induction attempts and B) TI duration (s). C) and D) Relaxed behavior pre and post exposure to the aerial predator in males (C) and in females (D). Data shown as mean and standard deviation. Star indicates a significant difference between genotypes (p<0.05).</p

Survival plots for the variable “first head movement” in the tonic.

<p>immobility (TI) test. Females (N = 31) and males (N = 46) are shown in separate plots. Solid lines show values for early stressed birds, and dotted lines for control. The treatment effect is significant in males but not in females. * : P< 0.05, NS = not significant.</p

Correlation of differential expression of overlapping genes at 28 (young) and 213 (adult) days of age.

<p>The values of the axes represent the fold changes in the differential expression when comparing early stressed males and females. The plots show the correlations of the differential fold changes of the genes overlapping on the top 1000 differentially expressed at each age. The values for the young birds were obtained immediately after a 30 min physical restraint period as explained in Material and Methods, and in the adults they were obtained five minutes after capture and removal from the home pen.</p

Social dominance in early stressed vs control male (N = 40) and female (N = 18) chickens.

<p>The figure shows the number of tested pairs in which either the early stressed or the control bird was scored as dominant. * : P< 0.05, NS = not significant.</p

Significantly enriched Gene Ontology (GO) terms among the 199 genes overlapping in the top 1000 list of differential gene expression in both 28 days old (post-restraint) and 213 days old male chickens.

<p>The table shows the GO-term assigned by the software DAVID, the number of genes in the category (Count) and the P-value (adjusted for false discovery rate) for the enrichment of the term.</p><p>Significantly enriched Gene Ontology (GO) terms among the 199 genes overlapping in the top 1000 list of differential gene expression in both 28 days old (post-restraint) and 213 days old male chickens.</p

Mean (+/- SEM) plasma levels of testosterone in males (N = 16) and estradiol in females (N = 16) at 16 weeks of age.

<p>(*): P = 0.06, NS = not significant.</p