A candidate protostellar object in the L1457 / MBM12 cloud

The association of young T Tauri stars, MBM12A, indicates that L1457 was forming stars not too long ago. With our study we want to find out whether or not there are still signs for ongoing star formation in that cloud. Using the Max-Planck-Millimeter-Bolometer MAMBO at the IRAM 30m telescope we obtained a map of about 8' by 8' centered on L1457 in the dust continuum emission at 230 GHz. Towards the most intense regions in our bolometer map we obtained spectra at high angular resolution in the CS (2-1) and the N2H+(1-0) lines using the IRAM 30m telescope. We find that the cold dust in L1457 is concentrated in several small cores with high H2 column densities and solar masses. The density profiles of the cores are inconsistent with a sphere with constant density. These cores are closer to virial equilibrium than the cloud as a whole. Data from the VLA and Spitzer archives reveal two point sources in the direction of one dust core. One of the sources is probably a distant quasar, whereas the other source is projected right on a local maximum of our dust map and shows characteristics of a protostellar object.Comment: 4 pages, 4 figures, accepted by Astronomy & Astrophysic

Manual de coleta de amostras de folhas, para diagnose nutricional, das principais frutíferas cultivadas no RS e em SC.

Métodos eficientes pra diagnosticar as condições nutricionais dos pomares e de recomendação de adubação.bitstream/item/33551/1/documento-142.pd

Leakage and spillover effects of forest management on carbon storage: theoretical insights from a simple model.

Leakage (spillover) refers to the unintended negative (positive) consequences of forest carbon (C) management in one area on C storage elsewhere. For example, the local C storage benefit of less intensive harvesting in one area may be offset, partly or completely, by intensified harvesting elsewhere in order to meet global timber demand. We present the results of a theoretical study aimed at identifying the key factors determining leakage and spillover, as a prerequisite for more realistic numerical studies.We use a simple model of C storage in managed forest ecosystems and their wood products to derive approximate analytical expressions for the leakage induced by decreasing the harvesting frequency of existing forest, and the spillover induced by establishing new plantations, assuming a fixed total wood production from local and remote (non-local) forests combined.We find that leakage and spillover depend crucially on the growth rates, wood product lifetimes and woody litter decomposition rates of local and remote forests. In particular, our results reveal critical thresholds for leakage and spillover, beyond which effects of forest management on remote C storage exceed local effects. Order of magnitude estimates of leakage indicate its potential importance at global scales

Identification of the main ubiquitination site in human erythroid α-spectrin

AbstractErythroid spectrin is the main component of the red cell membrane skeleton, which is very important in determining the shape, resistance to mechanical stresses and deformability of red cells. Previously we demonstrated that human erythroid α-spectrin is ubiquitinated in vitro and in vivo, and using recombinant peptides we identified on repeat 17 the main ubiquitination site of α-spectrin. In order to identify the lysine(s) involved in the ubiquitination process, in the present study we mutated the lysines by site-directed mutagenesis. We found that ubiquitination was dramatically inhibited in peptides carrying the mutation of lysine 27 on repeat 17 (mutants K25,27R and K27R). We also demonstrated that the correct folding of this protein is fundamental for its recognition by the ubiquitin conjugating system. Furthermore, the region flanking lysine 27 showed a 75% similarity with the leucine zipper pattern present in many regulatory proteins. Thus, a new potential ubiquitin recognition motif was identified in α-spectrin and may be present in several other proteins

Efficiency of transient transformation in tobacco protoplasts is independent of plasmid amount

An optimized protocol for the transient transformation of tobacco protoplasts mediated by polyethylene\u2013glycol (PEG) is here described. As expected, the quantitative b\u2013glucuronidase (Gus) activity driven by pCaMVGus was dependent on the amount of plasmid used. Nevertheless, we demonstrate by an immunodetection method that transformation efficiency did not depend on the amount of plasmid used but on the limitation imposed by cell competence. In fact, we obtained the same percentage of transformed cells (about 60%) using a wide range of plasmid concentrations (0.1\u201310 mg per test). Finally, we show that, when we used two plasmid types in a mixture at a concentration ranging from 0.1 to 10 mg for each, all transformed cells expressed proteins encoded by both plasmids. Transient expression and co-transformation experiments are routinely used methods and, probably, the major results from this work were assumed by many researchers in this field, but our data experimentally support this assumption

Endothelial cells, endoplasmic reticulum stress and oxysterols

Oxysterols are bioactive lipids that act as regulators of lipid metabolism, inflammation, cell viability and are involved in several diseases, including atherosclerosis. Mounting evidence linked the atherosclerosis to endothelium dysfunction; in fact, the endothelium regulates the vascular system with roles in processes such as hemostasis, cell cholesterol, hormone trafficking, signal transduction and inflammation. Several papers shed light the ability of oxysterols to induce apoptosis in different cell lines including endothelial cells. Apoptotic endothelial cell and endothelial denudation may constitute a critical step in the transition to plaque erosion and vessel thrombosis, so preventing the endothelial damaged has garnered considerable attention as a novel means of treating atherosclerosis. Endoplasmic reticulum (ER) is the site where the proteins are synthetized and folded and is necessary for most cellular activity; perturbations of ER homeostasis leads to a condition known as endoplasmic reticulum stress. This condition evokes the unfolded protein response (UPR) an adaptive pathway that aims to restore ER homeostasis. Mounting evidence suggests that chronic activation of UPR leads to cell dysfunction and death and recently has been implicated in pathogenesis of endothelial dysfunction. Autophagy is an essential catabolic mechanism that delivers misfolded proteins and damaged organelles to the lysosome for degradation, maintaining basal levels of autophagic activity it is critical for cell survival. Several evidence suggests that persistent ER stress often results in stimulation of autophagic activities, likely as a compensatory mechanism to relieve ER stress and consequently cell death. In this review, we summarize evidence for the effect of oxysterols on endothelial cells, especially focusing on oxysterols-mediated induction of endoplasmic reticulum stress