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04 avril 19241924/04/04 (A53).Appartient à l’ensemble documentaire : PoitouCh

Levels of TNF-α, caspase-3, IL-6, and superoxide dismutase in HEK293 cells lysates treated with vehicle, cisplatin (Cis, 10 μM), cisplatin+pioglitazone (PIO, 10 μM), cisplatin+fenofibrate (Feno, 10 μM), cisplatin+pioglitazone+fenofibrate, cisplatin+thalidomide (Thalid, 10 μM), and cisplatin+pioglitazone+fenofibrate+thalidomide.

<p>All drugs were incubated for 24 hr. Values are means±S.E.M. of 5 replicas. <b>*, ⁺, ^#</b>, ^π, ^$ and ^% denote significant difference (P<0.05) vs. control, cisplatin, cisplatin+pioglitazone, cisplatin+fenofibrate cisplatin+pioglitazone+fenofibrate and cisplatin+thalidomide values, respectively.</p

Blood urea nitrogen and serum creatinine concentration obtained from rats treated with saline (control), cisplatin (Cis, 5mg/kg, i.p.), cisplatin+pioglitazone (PIO, 2.5 mg/kg/day, orally), cisplatin+fenofibrate (Feno, 100 mg/kg/day, orally), cisplatin+pioglitazone+fenofibrate, cisplatin+pioglitazone+GW9662 (1 mg/kg/day, i.p), cisplatin+fenofibrate+GW6471 (1 mg/kg/day, i.p) and cisplatin+thalidomide (Thalid, 12.5 mg/kg/day, i.p).

<p>All drugs were administered 2 days before cisplatin and continued for 3 days thereafter. Values are means±S.E.M. of 8 observations. <b>*, ⁺, ^#</b> and ^π denote significant difference (P<0.05) vs. control, cisplatin, cisplatin+pioglitazone andcisplatin+fenofibratevalues, respectively.</p

Additive Renoprotection by Pioglitazone and Fenofibrate against Inflammatory, Oxidative and Apoptotic Manifestations of Cisplatin Nephrotoxicity: Modulation by PPARs - Fig 1

<p>Panel A, the total severity score for tubular damage obtained from H&E stained kidney sections (×400): score 0: normal histology, score 1: tubular cell swelling, brush border loss with up to 1/3 of the tubular profile showing nuclear loss, score 2: from 1/3 to 2/3 of the tubular profile showing nuclear loss, and score 3: more than 2/3 of the tubular profile showing nuclear loss. Shown also in panel A the graphical presentation of the total severity score for tubular necrosis of different treated groups. <b>*, ⁺, ^#</b> and ^π denote significant difference (P<0.05) vs. control, cisplatin, cisplatin+pioglitazone and cisplatin+fenofibratevalues, respectively. <b>Panel B</b>, hematoxylin and eosin stained photomicrographs (×100 and ×400) of kidneys obtained from rats treated with saline (control, image a), cisplatin (5mg/kg, i.p., image b), cisplatin+pioglitazone (2.5 mg/kg/day, orally, image c), cisplatin+fenofibrate (100 mg/kg/day, orally, image d), cisplatin+pioglitazone+fenofibrate (e), cisplatin+pioglitazone+GW9662 (1 mg/kg/day, i.p, image f), cisplatin+fenofibrate+GW6471 (1 mg/kg/day, i.p, image g) and cisplatin+thalidomide (12.5 mg/kg/day, i.p, image h). The black arrow shows glomerular atrophy whereas white arrows point to acute tubular necrosis, marked dilation of proximal convoluted tubules with vacuolation.</p

DNA fingerprinting, biological and chemical investigation of certain <i>Yucca</i> species

<p><i>Yucca aloifolia</i>, <i>Y. aloifolia variegata, Y. elephantipes</i> and <i>Y. filamentosa</i> were investigated. DNA sequencing was performed for the four plants and a genomic DNA fingerprint was obtained and provided. The cytotoxic activities against four human cancer cell lines were investigated. The ethanolic extracts of leaves of <i>Y. aloifolia variegata</i> prevailed, especially against liver cancer HepG-2 and breast cancer MCF-7. <i>In vivo</i> assessment of hepatoprotective activity in rats also revealed the hepatoprotective potential of the ethanolic extracts of the four plants against CCl₄- induced rats’ liver damage. Qualitative and quantitative analysis of the flavonoid and phenolic content of the promising species was performed using HPLC. The analysis identified and quantified 18 flavonoids and 19 phenolic acids in the different fractions of <i>Y. aloifolia variegat</i>a, among which the major flavonoids were hesperidin and kaemp-3-(2-<i>p</i>-coumaroyl) glucose and the major phenolic acids were gallic acid and protocatechuic acid.</p