Increased quenching of 1,2′-DNA fluorescence at higher dye loading concentrations (DLCs) in strain 3-AG100.

<p>Depicted are relative fluorescence units (RFU) of deenergized cells preloaded with 1,2′-DNA after resuspension in fresh PPB. All experiments were done in triplicate. The error bars show the standard deviation.</p

Representative 1,2′-DNA efflux curves of 3-AG100-derived AcrB binding pocket mutants.

<p>After preloading with 4 µM 1,2′-DNA the cells were energized at 100 s with 50 mM glucose. Fluorescence intensity is given as relative fluorescence units (RFU) with preenergization levels adjusted to 100 RFU.</p