Assessment of the relationship amongst the function and activity of the left ventricle and kidneys function, the uric acid and TGF-β, VEGF in patients with untreated hypertension

Wstęp Celem pracy była próba ustalenia związku między zmianami morfologicznymi i czynnościowymi w układzie sercowo-naczyniowym a czynnością nerek, kwasem moczowym oraz TGF-&#946; i VEGF. Materiał i metody Badaniem objęto 30 pacjentów (9 kobiet i 21 mężczyzn) w wieku 18-50 lat z dotychczas nieleczonym, pierwotnym nadciśnieniem tętniczym. Grupę kontrolną stanowiło odpowiednio dobranych 16 zdrowych ochotników. Wyniki Chorzy z nadciśnieniem tętniczym charakteryzowali się wyższymi wartościami ciśnienia tętniczego w porównaniu z grupą kontrolną: skurczowego(148,88 &#177; 6,39 mm Hg vs. 115 &#177; 9,66 mm Hg), rozkurczowego (95 &#177; 4,35 mm Hg vs. 68,44 &#177; &#177; 8,11 mm Hg) i ciśnienia tętna (53,83 &#177; 6,65 mm Hg vs. 46,56 &#177; &#177; 5,39 mm Hg). Badane grupy nie różniły się pod względem wydalania NAG oraz stężenia TGF-&#946; i VEGF w surowicy. W grupie osób z nadciśnieniem tętniczym wystąpiła istotnie statystycznie dodatnia korelacja między stężeniem VEGF a ciśnieniem tętna (r = 0,41, p < 0,03). Jedynie w analizie wieloczynnikowej wykazano, że LVMI zależy od GFR i wydalania NAG (r = 0,66, p < 0,003), nie wykazano korelacji między kwasem moczowym a czynnością układu sercowo-naczyniowego u pacjentów z nadciśnieniem tętniczym. Wnioski W przedstawionym badaniu sugeruje się, że zarówno VEGF, jak i TGF-&#946; we wczesnym okresie nadciśnienia tętniczego mogą odgrywać rolę w rozwoju powikłań ze strony układu sercowo-naczyniowego.Background Aim of the study was to evaluate the relationship among morphological and functional changes in the cardiovascular system and function of kidneys, uric acid as well as TGF-&#946; and VEGF. Material and methods We examined 30 ambulatory patients (9 Female and 21 Male), aged 18-50 years with nontreated essential hypertension (HT). The control group was of 16 healthy volunteers with age and gender matched (C). Results In hypertension (HT) blood pressure was higher than control (C): systolic (148.88 &#177; 6.39 mm Hg vs. 115 &#177; &#177; 9.66 mm Hg), diastolic (95 &#177; 4.35 mm Hg vs. 68.44 &#177; &#177; 8.11 mm Hg), pulse pressure (53.83 &#177; 6.65 mm Hg vs. 46.56 &#177; 5.39 mm Hg). We did not reveal significant differences in serum concentration of TGF-&#946; and VEGF between the examined and the control group. In HT the significant positive correlation between VEGF and pulse pressure (r = 0.41, p < 0.03) was observed. Only multifactorial correlation showed that LVMI was related to GFR and NAG excertion (r = 0.66, p < 0.003) but no correlation was found between uric acid and function of structure cardiovascular system in hypertensive patient. Conclusions Our study suggested that both VEGF and TGF-&#946; in the early period of arterial hypertension can take a part in development of cardiovascular system complications and kidney function

Antimicrobial resistance and virulence genes in staphylococci isolated from aviary capercaillies and free-living birds in south-eastern Poland

The current study characterises Staphylococcus bacteria recovered from dead free-living birds and captive capercaillies kept in south-eastern Poland. The results provide novel information about the antimicrobial resistance phenotype/genotype and the virulence profile of these bacteria

Biofilm-Formation Ability and the Presence of Adhesion Genes in Coagulase-Negative Staphylococci Isolates from Chicken Broilers

The aim of the study was to analyze the biofilm-production capacity of 87 coagulase-negative Staphylococcus strains (CoNS) isolated from broiler chickens and to determine the occurrence of biofilm-associated genes. The biofilm production capacity of staphylococci was assessed using the microtiter plate method (MTP), and the frequency of genes was determined by PCR. The ability to form a biofilm in vitro was shown in 79.3% of examined strains. Strong biofilm capacity was demonstrated in 26.4% of strains, moderate capacity in 25.3%, weak capacity in 27.6%, and a complete lack of biofilm production capacity in 20.7% of strains. The icaAB gene responsible for the production of extracellular polysaccharide adhesins was detected in 6.9% of strains. The other four genes, i.e., bap (encoding biofilm-associated protein), atlE (encoding cell surface protein exhibiting vitronectin-binding activity), fbe (encoding fibrinogen-binding protein), and eno (encoding laminin-binding protein) were detected in 5.7%, 19.5%, 8%, and 70.1% of strains, respectively. Demonstration of genes that play a role in bacterial biofilm formation may serve as a genetic basis to distinguish between symbiotic and potentially invasive coagulase-negative staphylococcal strains