Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats

Selenium nanoparticles (SeNPs) are fast becoming a key instrument in several applications such as medicine or nutrition. Questions have been raised about the safety of their use. Male rats were fed for 28 days on a monodiet containing 0.5, 1.5, 3.0 and 5.0 mg Se/kg. Se content in blood and liver, liver panel tests, blood glucose, total antioxidant capacity (TAC), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analysed. Liver and duodenum were subjected to histopathology examination. The weight gain of rats showed no differences between tested groups. Se content in blood was higher in all treated groups compared to the control group. The liver concentration of Se in the treated groups varied in the range from 222 to 238 ng/g. No differences were observed in the activity of AST (aspartate aminotransferase), ALP (alkaline phosphatase) and TAS (total antioxidant status). A significant decrease in ALT activity compared to the control group was observed in the treated groups. GPx activity varied from 80 to 88 U/mL through tested groups. SOD activity in liver was decreased in the SeNP-treated group with 5 mg Se/kg (929 ± 103 U/mL). Histopathological examination showed damage to the liver parenchyma and intestinal epithelium in a dose-dependent manner. This study suggests that short-term SeNP supplementation can be safe and beneficial in Se deficiency or specific treatment

Composition of feed mixture.

The aim of this experiment was to find out whether the taurine supplementation in daily ration had an effect on quantity or quality of Duroc boar ejaculate. The experiment duration was from June to August, when it could assumed the possible occurrence of heat stress. For the study was chosen 12 Duroc boars of approximately the same age and condition. The control group of 6 Duroc boars was fed only by basic diet and the experimental group of 6 Duroc boars was fed by the same basic diet with supplementation of 15 g taurine/boar/day. Ejaculate was collected once a week by hand glowed technique. From ejaculate parameters were monitored volume of ejaculate, sperm concentration, total amount of sperm, morphologically abnormal sperm, taurine concentration and GSH/GSSH concentration. From microscopic analysis, results were statistically significant in motility in June and July (P</div

Average and maximum temperature and relative humidity measured during the experiment.

Average and maximum temperature and relative humidity measured during the experiment.</p

Concentration of GSSG (μg/ml) and GSH (μg/ml) in ejaculate.

Concentration of GSSG (μg/ml) and GSH (μg/ml) in ejaculate.</p

Volume of ejaculate (ml) and Concentration of sperm (10⁶/ml).

Volume of ejaculate (ml) and Concentration of sperm (106/ml).</p

Total rate of sperm (10⁹/ml) and Motility of sperm (%).

Total rate of sperm (109/ml) and Motility of sperm (%).</p

Morphologically abnormal sperm (%) and Concentration of taurine in ejaculate (μg/ml).

Morphologically abnormal sperm (%) and Concentration of taurine in ejaculate (μg/ml).</p