Time kinetic of body weight and food intake in wild-type versus mdr1ab^-/- mice.

<p>The weights were recorded weekly from weaning to 35-weeks of age for male (Fig. 1A) or female (Fig. 1B) wild-type (open symbols) and mdr1ab^-/- (close symbols) mice fed a standard chow diet. Results are means ± s.e.m of 5 to 10 animals. Differences are considered significant when *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001. Daily food intakes of males (Fig. 1C) and females (Fig. 1D) were calculated weekly by monitoring the total food consumption for wild-type (open symbols) and mdr1ab^-/- (close symbols) mice, measured as mean food consumption for two to three mice caged together. Results are expressed per gram of body weight and are the means of 3 values per subgroup.</p

Liver and adipose tissue contributions to the body weight in wild-type versus mdr1ab^-/- mice.

<p>Mice were sacrificed at 25 and 35 weeks of age and discrete fat pads from five anatomical sites were dissected and weighed. WAT refers to white adipose tissue. Total WAT weight represents the sum of the data reported for the four WAT sites considered in the study. Body weight is expressed in gram and tissue weights are expressed as percentage of body weight. F is the ratio of mdr1ab^-/- versus wild-type. Results are means ± s.e.m. Statistical relevance of data is assessed by <i>p</i> values.</p>a<p><i>p</i><0.05;</p>b<p><i>p</i><0.01.</p

Influence of high fat diet (HFD) on hepatic triglyceride concentrations in wild-type and mdr1ab^-/- mice.

<p>Mice were fed with HFD during 25 weeks. Triglycerides have been quantified in liver samples from 15- or 25-week old wild-type (open bars) and mdr1ab^-/- (solid bars) mice. Results are expressed as nmol per mg of liver and are means ± s.e.m of 3 values at 15-week old and 5 at 25-week old. Differences are considered significant when *, <i>p</i><0.05; **, <i>p</i><0.01.</p

Morphological comparison and liver lipid content in wild type and mdr1ab^-/- males.

<p>2A. Whole body. 2B. Respective livers at 35 weeks of age. 2C. Oil red O staining of liver sections. Livers were collected from 25- or 35-week old mice and kept frozen in OCT at −80°C until analysis. 2D. Lipid content of liver. Total cholesterol (upper histogram) and triglycerides (lower histogram) in liquid-frozen liver samples from 25- and 35- week old mice, were quantified by liquid chromatography in wild-type (open bars) and mdr1ab^-/- (solid bars) males. Results are expressed as means ± s.e.m of 5 to 7 mice. Differences are considered significant when <i>p<</i>0.05. ***, <i>p</i><0.001.</p

Analysis of lipids and xenobiotics target genes expression in the liver.

<p>Relative gene expressions of <i>scd-1</i>, <i>fasn</i>, <i>cd36</i>, <i>acox</i>, <i>cyp2B10</i> and <i>cyp3A11</i> were quantified in liver samples from 12-week old (Fig. 5A) and 25-week old (Fig. 5B) mice by real-time time PCR. Similarly, <i>scd-1, cyp7a1</i> and <i>pltp</i> expressions were quantified in 35-week old mice (Fig. 5C); wild-type (open bars) and mdr1ab-/- (solid bars). Values were normalized to TBP gene and expressed as arbitrary unit. Values shown are means ± s.e.m (n = 3). Differences are considered significant when <i>p</i><0.05. *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001.</p

Lipid content in bile of wild-type and mdr1ab^-/- mice.

<p>Cholesterol and bile acids were quantified in bile in wild-type (open bars) and mdr1ab^-/- (solid bars) 35-week old male mice. Results are expressed as means ± s.e.m of 5 mice. Differences are considered significant when *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001.</p