Susceptibility in vitro of canine methicillin-resistant and -susceptible staphylococcal isolates to fusidic acid, chlorhexidine and miconazole: opportunities for topical therapy of canine superficial pyoderma

OBJECTIVES: Increasing multidrug resistance amongst canine pathogenic staphylococci has renewed interest in topical antibacterial therapy for skin infections in the context of responsible veterinary prescribing. We therefore determined the activity in vitro of three clinically relevant topical agents and synergism between two of them against Staphylococcus pseudintermedius and Staphylococcus aureus. METHODS: The MICs of fusidic acid (n = 199), chlorhexidine (n = 198), miconazole (n = 198) and a 1:1 combination of miconazole/chlorhexidine (n = 198) were determined for canine isolates [50 MRSA and 49 methicillin-resistant S. pseudintermedius (MRSP), 50 MSSA and 50 methicillin-susceptible S. pseudintermedius (MSSP)] collected from the UK and Germany using an agar dilution method (CLSI VET01-A4). Fractional inhibitory concentration (FIC) indices were calculated to assess the interaction of miconazole with chlorhexidine. RESULTS: MICs of each drug/combination were significantly (P < 0.0005) higher for S. aureus when compared with S. pseudintermedius. Most strains (n = 172) had an MIC of fusidic acid of ≤0.03 mg/L (MIC ≥64 mg/L, n = 5 MRSA). All strains had MICs of chlorhexidine of 0.5–4 mg/L, except for one MRSA (MIC = 8 mg/L). All but four strains had MICs of miconazole of 1–4 mg/L (MIC = 16 mg/L, n = 3; MIC = 256 mg/L, n = 1). Miconazole/chlorhexidine (1:1 ratio) had a synergistic effect against 49/50 MRSA, 31/50 MSSA, 12/49 MRSP and 23/49 MSSP. CONCLUSIONS: Since the majority of these staphylococci, including methicillin-resistant isolates, had MICs that should be readily exceeded by topical skin application of these agents, their therapeutic efficacy for canine superficial pyoderma should be assessed. The synergistic interaction shown in vitro supports further clinical evaluation of miconazole/chlorhexidine combination therapy for staphylococcal infection

Contained-Reflected Megaturbidites of the Marnoso-arenacea Formation (Contessa Key Bed) and Helminthoid Flysches (Northern Apennines, Italy) and Hecho Group (South-Western Pyrenees)

Contained-reflected beds deposited by fully-ponded or partially-reflected turbidity currents are important because their correct evaluation can give important indications on the degree of basin confinement and on the type, size and orientation of the morphological obstacle. Through a detailed facies analysis of various significant megabeds in the Marnoso-arenacea Formation, including the Contessa key bed, in the helminthoid flysches in the northern Apennines (Italy) and in the Pyrenees (megaturbidite MT5), this work proposes a depositional model that is well consistent with the recent experimental data available in the literature, discussing their strengths and limits. The Contessa and flysch megabeds fit very well with the experimental conditions because they are deposited in narrow and elongated confined basins characterized by axial flows. Indeed, in the proposed model, it is possible to recognize facies deposited by: 1) a basal underflow directed towards the bounding slope (Facies A), 2) an intermediate part of the flow characterized by lateral deflections (facies B1), 3) an upper well-developed reversing flow (facies B2) and 4) an uppermost residual reversing flow recording the final collapse of the fine-grained suspended load forming a poorly-sorted slurry facies C and a very thick mudstone unit D. Facies A, B1 and B2 are usually separated by very thin fine-grained muddy drapes rich in carbonaceous matter, which can be traced throughout the basin. These drapes - very common in contained and confined beds in these settings - can be related to internal density surfaces, along which decoupling processes, separating underflows from reversing overflows, can easily occur. Conversely, as the MT5 is characterized by a source transversal to an elongated narrow basin, the large flow volume versus basin capacity hinders the generation of reversing flows and rebound layers favoring the formation of fully-ponded pulsating overflows able to deposit alternations of laminated and massive units. This facies type can be observed in the basins that are characterized by axial flows only near the basin margins where the pulsating collapse of the reversing flow can dominate. This study shows that the integration of detailed field studies are essential to validate experimental data from an applicative point of view

Caracterização de grande número de amostras de madeira de pinus visando aplicação em seleção genômica ampla.

Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos

One-step replica symmetry breaking solution for a highly asymmetric two-sublattice fermionic Ising spin glass model in a transverse field

The one-step replica symmetry breaking (RSB) is used to study a two-sublattice fermionic infinite-range Ising spin glass (SG) model in a transverse field $\Gamma$. The problem is formulated in a Grassmann path integral formalism within the static approximation. In this model, a parallel magnetic field $H$ breaks the symmetry of the sublattices. It destroys the antiferromagnetic (AF) order, but it can favor the nonergodic mixed phase (SG+AF) characterizing an asymmetric RSB region. In this region, intra-sublattice disordered interactions $V$ increase the difference between the RSB solutions of each sublattice. The freezing temperature shows a higher increase with $H$ when $V$ enhances. A discontinue phase transition from the replica symmetry (RS) solution to the RSB solution can appear with the presence of an intra-sublattice ferromagnetic average coupling. The $\Gamma$ field introduces a quantum spin flip mechanism that suppresses the magnetic orders leading them to quantum critical points. Results suggest that the quantum effects are not able to restore the RS solution. However, in the asymmetric RSB region, $\Gamma$ can produce a stable RS solution at any finite temperature for a particular sublattice while the other sublattice still presents RSB solution for the special case in which only the intra-sublattice spins couple with disordered interactions.Comment: 11 pages, 8 figures, accepted for publication in Phys. Rev.

Formas de nitrogênio e tipo de substrato nos parâmetros de absorção de fósforo pelo milho-doce.

Foram realizados dois experimentos em casa de vegetação, em solo e em solução nutritiva, para testar, em milho-doce, o modelo matemático de Cushmao-Barber, utilizado para predizer a absorção de fósforo. Foram efetuadas análises de sensibilidade, para estudar o efeito, na absorção, da mudança de cada um dos parâmetros. A área superficial de raízes teve grande efeito na absorção de fósforo, em ambos os substratos. independente da fonte de nitrogênio. Verificou-se uma interação substrato x fonte de nitrogênio, embora a amplitude de absorção de fósforo fosse a mesma em todos os casos. Os resultados obtidos mostram que o suprimento de fósforo pelo substrato não foi o passo limitante na absorção desse nutriente pelas plantas