Rhesus negative pregnant women in a traditional birth home in Abeokuta, Nigeria

In a survey of 200 pregnant women (mean age 24 years) attending a traditional birth home (TBH) in Abeokuta, Nigeria, 19 (9.5%) were found to be rhesus negative, 8 (42.1%) of which were primigravidae while 11 (57.9%) were multigravidae. 87.5% of the Rhesus negative primigravidae delivered at the TBH without being given the post partum injection of anti-D-gama globulin within 72 h of delivery, thereby having their systems likely sensitized (if baby is rhesus positive) against subsequent pregnancies involving Rhesus positive fetuses. Of the multigravidae involved in this study 27.3% women delivered live babies at the traditional birth home while the remaining 72.7% women were not seen again at the TBH.  One woman who has had an induced abortion and was carrying a second pregnancy lost the pregnancy in the course of this study. The knowledge of these women (who were mostly without formal education) on their haematological status is nil. The need to educate the public, especially women patronizing TBH, on the rhesus problem is recommended. (African Journal of Biotechnology: 2003 2(8): 241-243

Population dynamics of indoor sampled mosquitoes and their implication in disease transmission in Abeokuta, south-western Nigeria

Background & objectives: A longitudinal study was carried out to investigate the speciescomposition, seasonal abundance, parity and feeding preference of indoor sampled mosquitoes inAbeokuta, south-western Nigeria.Methods: The mosquitoes were sampled weekly from five stratified locations using Center forDisease Control (CDC) light-traps between August 2005 and July 2006. The mosquitoes wereexamined for abdominal condition and dissected for age composition. Microscopic and precipitintechniques were also employed for the determination of host blood source.Results: A total of 2969 mosquitoes which belong to 10 species of mosquitoes were collectedduring the study period. Mansonia africana (35.65%) constituted the most abundant species followedby Culex quinquefasciatus (32.23%) and Anopheles gambiae complex (13.52%). Other species indecreasing order of abundance were Coquilletidia maculipennis (8.2%), Aedes albopictus (5.9%),Ae. aegypti (1.93%), M. uniformis (1.81%), Cx. duttoni (0.25%), Cx. tigripes (0.25%) and An.funestus (0.25%). Seasonal abundance revealed a significant difference (p <0.05) in the populationof mosquito vectors collected during the wet season as compared to the dry season and theirabundance was positively correlated with rainfall. The results showed that the majority of thevector species collected were unfed and nulliparous. Moreover, the blood meal test was positive forhuman blood.Conclusion: The preponderance of mosquitoes observed in the study is of public health concernsince they serve as vectors of most tropical diseases including malari

Health knowledge and hygiene behaviors among schoolchildren in relation to Ascariasis in Ogun State, Nigeria.

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MONRATE: A descriptive tool for calculation and prediction of re-infection of Ascaris lumbricoides (Ascaridida: Ascarididae)

The study presents an interactive descriptive tool (MONRATE) for calculating and predicting reinfection rates and time of Ascaris lumbricoides following mass chemotherapy. The implementation was based on the theoretical equation published by Hayashi in 1977, for time-prevalence: Y=G [1-(1-X)N-R] as modified by Jong-Yil in 1983. Using the Psuedo-Code of the MONRATE tool, the calculated monthly reinfection rates (X) for the LGAs are (names are locations in Nigeria in a region predominately populated by the Yoruba speaking tribes of Nigeria whose traditional occupations are agriculture and commerce): Ewekoro (1.6 %), Odeda (2.3 %), Ado-odo/Otta (2.3 %), Ogun Waterside (3.8 %) and Obafemi/Owode (4.2 %). The mathematical mean of ‘X’ values in the study areas for Ogun State was 2.84. The calculated reinfection time (N months) for the LGAs are varied such as Ado-odo/Otta (12.7), Ogun Waterside (21.8), Obafemi/Owode (22.92), Odeda (25.45), and Ewekoro (25.9). The mean value for N in Ogun State was 21.75. The results obtained from MONRATE were compared with those obtained using the mathematical equation and found to be the same. Rev. Biol. Trop. 55 (3-4): 755-760. Epub 2007 December, 28

MONRATE, a descriptive tool for calculation and prediction of re-infection of Ascaris lumbricoides

The objective of the study was to develop an interactive and systematic descriptive tool, MONRATE for calculating and predicting reinfection rates and time of Ascaris lumbricoides   following mass chemotherapy using levamisole. Each pupil previously treated was retreated 6 or 7 months after the initial treatment in Ogun State, Nigeria. The implementation was based on the theoretical equation for time-prevalence: Y = G [1 -(1-X)N-R]. Using the Psuedo-Code of the MONRATE tool, the calculated monthly reinfection rates (X) for the LGAs were 1.6% in Ewekoro, 2.3% in Odeda, 2.3% in Ado-odo/Otta, 3.8% in Ogun Waterside and 4.2% in Obafemi/Owode. The mathematical mean of 'X' values in the study areas for Ogun State was 2.84. The calculated reinfection time (N months) for the LGAs varied such as Ado-odo/Otta (12.7), Ogun Waterside (21.8), Obafemi/Owode (22.92), Odeda (25.45), and Ewekoro (25.9). The mean value for N in Ogun State was 21.75. The results obtained from MONRATE were compared with those obtained using the mathematical equation and were found to be the same but MONRATE was faster in computation and more accurate. It is concluded that MONRATE is a veritable tool that can be used in the execution of control programme involving mass treatment against A. lumbricoides

Cultivation and multiplication of viable axenic trypanosoma vivax in vitro and in vivo

Trypanosoma vivax was isolated from the blood of an infected laboratory mouse, washed and introduced into the prepared culture media, ME-99 and minimum essential medium (MEM), both containing laboratory prepared (commercial) horse serum and antibiotics (streptomycin and penicillin). The cultures were monitored in vitro for 12 days. There was an initial decline in parasitaemia in the first 48 h in both media, which later picked up to reach the peak of about 1.10 x 102 parasites/ml in ME-99 on day 5 and 6.2 x 10 parasites/ml in MEM on day 4. Thereafter, the parasites number tapered off to reach zero on day 9 in ME-99 and day 10 in MEM. No growth was recorded in the control, which contained normal saline (pH 7), horse serum and antibiotics. The result of the in vivo culture showed a different trend when compared to the in vitro. Multiplication was tremendous with a peak of about 3 x 10 parasites/ml of blood on day 22 (high inoculum) and day 24 (low inoculum) post infection. The slender trypomastigote parasites recovered in the in vitro culture was short and had a long, free flagellum and measured 23-25 um while that of the in vivo culture was long, slender, clongated, torpedo shaped body measuring between 30 and 32 um. During the course of the in vivo culture congenital transmission of trypanosomes was observed. The in vitro attenuated parasite conferred a degree of protection to 25% of the mice that were later infected with viable parasites indicating possible prophylactic effect of in vitro attenuated parasites. The study showed that T. vivax could not be cultured in large numbers on their own axenically in MEM and ME-99. However, ME-99 can be said to be more suitable compared to MEM for axenic cultivation of T. vivax as a result of the additional nutrients supplied by the tissue culture medium 199 present in medium ME-99. Also, the parasites multiplied better in vivo compared to the in vitro study, which could mean that the best means of culturing trypanosomes still remains the in vivo method

Morphometric studies on Culex quinquefasciatus and Mansonia africana (Diptera: Culicidae) in Abeokuta, south-western Nigeria

Some of the important mosquito species are generally sympatric in terms of their geographical distributions, and are difficult to identify based solely on examination of adult females using the available morphological keys. In this study, adult mosquitoes were collected indoors in Abeokuta in south-western Nigeria between August 2005 and July 2006 using Center for Disease Control light traps. The objective was to investigate possible morphological differences in populations of Culex quinquefasciatus and Mansonia africana. Six morphological characters namely, wing length, antennal length, proboscis length, foreleg length, mid leg length and hind leg length were measured in the two species. A total of 868 Cx quinquefasciatus and 962 M. africana were collected during the study period. The mean length was observed to be higher in most characters during the wet season than the dry season but the variation was not statistically signifi cant (P> 0.005). In M. africana, the antennal length, proboscis length, foreleg length and midleg length showed one peak each. The wing length exhibited three peaks while hind leg length showed two peaks. For Cx quinquefasciatus, the antennal length, proboscis length, foreleg length, mid leg length and hind leg length indicated one peak. The wing length however showed three peaks. Each of the peaks observed in wing length and hind leg length of M. africana and wing length of Cx quinquefasciatus was assumed to be a specific population. The coefficient of differences (CDs) for each population indicated the presence of three populations in M. africana (CD > 1.28). However, the hind leg length showed that the two populations were the same (CD< 1.28). Three populations were also obtained from CD for wing leg length of Cx quinquefasciatus (CD>1.28). Other characters indicated the presence of only one population. The results therefore underscore the need to investigate the status of the two species in relation to species complex

Mosquito larval habitats and public health implications in Abeokuta, Ogun State, Nigeria

The larval habitats of mosquitoes were investigated in Abeokuta, Nigeria in order to determine the breeding sites of the existing mosquito fauna and its possible public health implications on the residents of the City. The habitats were sampled between August 2005 and July 2006 using plastic dippers and a pipette. The habitats were grouped as ground pools/ponds, gutters/open drains, tyres, domestic containers and treeholes/ leaf axils. Ten species of mosquitoes were encountered in the five habitats namely Mansonia africana , M. uniformis, Culex quinquefasciatus , Aedes aegypti , Ae. albopictus, Ae. vittatus, Cx tigripes, Anopheles gambiae s.l., An. funestus and Eretmapodite clnysogaster . Ae. aegypti bred in all the habitats sampled while Cx quinquefasciatus bred in four habitats except tree holes/leaf axils. An. gambiae s.l and Ae. albopictus occurred in three habitats while other species bred only in one or two habitats. Ground pools and domestic containers recorded the highest number of species followed by gutters/open drains. Tree holes/leaf axils was the least preferred habitat with the lowest number of species occurrence. However, statistical analysis revealed non-significant difference in species occurrence in the five habitats. The availability of the habitats to support the breeding of Aedes, Culex and Anopheles, which are known vectors of urban yellow fever, lymphatic filariasis and malaria suggest that the residents of Abeokuta City are at risk of mosquito-borne diseases. It is important that residents of the City are enlighten on the environmental factors that contribute to mosquito breeding and that the Government should institute proper sanitation measures to reduce mosquito breeding sites