Portable, High-Bandwidth Frequency-Domain Photon Migration Instrument for Tissue Spectroscopy

We describe a novel frequency-domain photon migration instrument employing direct diode laser modulation and avalanche photodiode detection, which is capable of noninvasively determinating the optical properties of biological tissues in near real time. An infinite medium diffusion model was used to extract absorption and transport scattering coefficients from 300-kHz to 800-MHz photon-density wave phase data. Optical properties measured in tissue-simulating solutions at 670 nm agreed to within 10% of those expected

A High-Bandwidth Frequency-Domain Photon Migration Instrument for Clinical Use

We have developed a high-bandwidth frequency-domain photon migration (FDPM) instrument which is capable of noninvasively determining the optical properties of biological tissues in near-real-time. This portable, inexpensive, diode-based instrument is unique in the sense that we employ direct diode laser modulation and avalanche photodiode detection. Diffusion models were used to extract the optical properties (absorption and transport scattering coefficients)of tissue-simulating solutions.from the 300 kHz to I GHz photon density wave data

Frequency-Domain Photon Migration in Turbid Media

An analytical model is presented for the propagation of diffuse photon density waves in turbid media. The frequency- and wavelength-dependence of photon density waves are measured using Frequency-domain Photon Migration (FDPM). Media optical properties, including absorption, transport, and fluorescence relaxation times are calculated from experimental results

A Cosmological Three Level Neutrino Laser

We present a calculation of a neutrino decay scenario in the early Universe. The specific decay is \nu_{2} \to \nu_{1} + \phi, where \phi is a boson. If there is a neutrino mass hierarchy, m_{\nu_{e}} < m_{\nu_{\mu}} < m_{\nu_{\tau}}, we show that it is possible to generate stimulated decay and effects similar to atomic lasing without invoking new neutrinos, even starting from identical neutrino distributions. Under the right circumstances the decay can be to very low momentum boson states thereby producing something similar to a Bose condensate, with possible consequences for structure formation. Finally, we argue that this type of decay may also be important other places in early Universe physics.Comment: 7 pages, RevTex, due for publication in Phys. Rev. D, April 15 issu

Optical Properties of Human Uterus at 630 nm

The optical properties of normal and fibriotic human uteri were determined using frequency-domain and steady-state techniques

Phase Velocity Limit of High-Frequency Photon Density Waves

In frequency-domain photonmigration (FDPM), two factors make high modulation frequencies desirable. First, with frequencies as high as a few GHz, the phase lag versus frequency plot has sufficient curvature to yield both the scattering and absorption coefficients of the tissue under examination. Second, because of increased attenuation, highfrequency photon density waves probe smaller volumes, an asset in small volume in vivo or in vitro studies. This trend toward higher modulation frequencies has led us to reexamine the derivation of the standard diffusion equation (SDE)from the Boltzman transport equation. We find that a second-order time-derivative term, ordinarily neglected in the derivation, can be significant above 1GHzfor some biological tissue. The revised diffusion equation, including the second-order time-derivative, is often termed the PI equation. We compare the dispersion relation of the PI equation with that of the SDE. The PI phase velocity is slower than that predicted by the SDE; in fact, the SDE phase velocity is unbounded with increasing modulation frequency, while the PI phase velocity approaches c/sqrt(3) in the high frequency limit. We emphasize that the phase velocity c/sqrt(3) is attained only at modulation frequencies with periods shorter than the mean time between scatterings of a photon, a frequency regime that probes the medium beyond the applicability of diffusion theory. Finally we caution that values for optical properties deduced from FDPM data at high frequencies using the SDE can be in error by 30% or more

Targeted delivery of bleomycin to the brain using photo-chemical internalization of Clostridium perfringens epsilon prototoxin

Cells infiltrating into normal brain from malignant brain tumors are protected by the blood brain barrier (BBB) which prevents the delivery and limits the effects of anti-tumor agents. We have evaluated the ability of photochemical internalization (PCI) to limit the effects of an agent known to broadly open the BBB to a target region of the brain. The PCI-based relocation and activation of macromolecules into the cell cytosol has the advantage of minimal side effects since the effect is localized to the area exposed to light, allowing the access of chemotherapeutic agents only to these regions. Non tumor bearing inbred Fisher rats were treated with photosesitizer, and a nontoxic intraperitoneal dose of Clostridium perfringens epsilon prototoxin (ETXp) followed by light exposure. Post-contrast T1 MRI scans were used to monitor the degree BBB disruption. F98 tumor cells were implanted into the brains of other animals that were subsequently treated 24 h later with ETXp-PCI BBB opening followed by the i.p. administration of bleomycin (BLM). PCI delivery of ETXp at low fluence levels demonstrated significant MRI enhancement. No effect on the BBB was observed if photosesitizer and light was given in the absence ETXp. The survival of animals implanted with F98 tumor cells was significantly extended following ETXp-PCI BBB opening and BLM therapy compared to controls. PCI delivered ETXp was effective in opening the BBB in a limited region of the brain. ETXp-PCI mediated BBB opening clearly increased the efficacy of BLM therapy

Probing neutrino decays with the cosmic microwave background

We investigate in detail the possibility of constraining neutrino decays with data from the cosmic microwave background radiation (CMBR). Two generic decays are considered \nu_H -> \nu_L \phi and \nu_H -> \nu_L \nu_L_bar \nu_L. We have solved the momentum dependent Boltzmann equation in order to account for possible relativistic decays. Doing this we estimate that any neutrino with mass m > 1 eV decaying before the present should be detectable with future CMBR data. Combining this result with other results on stable neutrinos, any neutrino mass of the order 1 eV should be detectable.Comment: 8 pages, 4 figures, to appear in Phys. Rev.