Additional file 3: Figure S2. of Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease

In vivo experimental schemes. (Top) In the first set of experiments mice were treated with CCl4 and 3 hours later pre-treated ucMSC (untreated, IFN-γ, IFN-β, TGFβ, starvation, vitamin B6, Starv + VitB6, RA and MC), which were labeled with Qtracker605 beads, were infused IV. Four hours after ucMSC infusion the mice were sacrificed and prepared for imaging. (Bottom) In the second set of experiments mice were sacrificed 72 hours after CCL4 injection and serum was collected. (TIF 7779 kb

El Diario de Pontevedra : periódico liberal: Ano XXIII Número 6606 - 1906 abril 27

Ex vivo experimental scheme. UcMSC were pre-treated for 3 days. Medium was then refreshed and the cells were left overnight in the incubator. The following day, livers were collected from healthy C57BL/6 mice and directly after isolation cut into slices (diameter = 1 cm and thickness = 150 μm) and placed in the wells on top of the pre-treated ucMSC in the presence of LPS. The liver slices were left overnight in the incubator on a shaker (50 rpm). After 24 hours, supernatant and liver slices were harvested. (TIF 4600 kb