Evaluation of Fungicides, Soil Amendment Practices and Bioagents against Fusarium solani-Causal Agent of Wilt Disease in Chilli

Chilli is affected by the wilt disease caused by Fusarium solani, under irrigated conditions. In absence of resistant cultivars, the disease needs to be controlled by management practices. In vitro evaluation of six fungicides by Poisoned Food Technique showed that a combination of carbendazim+mancozeb was effective in inhibiting mycelial growth (93.6%), followed by Carbendazim alone (92.4%). In vivo soil drench using the same fungicides proved effective in controlling the pathogen. Integration of different treatments, including seedling dip, with Carbendazim, addition of vermicompost, drenching with fungicide, and application of Trichoderma viride was found to be effective in managing the disease, in comparison to individual treatments

Effect of Various Plant Extracts on Dry Root Rot of Chillies Caused by Sclerotium rolfsii

Eight different plant extracts were evaluated in vitro against Sclerotium rolfsii causing dry root rot in chillies. Among these, leaf extract of neem (Azadirachta indica) caused maximum inhibition of mycelial growth (80.74%), followed by periwinkle Vinca rosea (78.8%) and bottlebrush (Callistemon, 74.8%) respectively. Sclerotial production was inhibited to an extent of 11% and the inhibition caused was maximum with neem extract, followed by Polyalthia longifolia and V. rosea extracts. Though sclerotial germination was inhibited by 30% to 95% in various treatments, the most effective treatment was that of neem leaf extract (95%), followed by ginger extract (92%)

Compatibility of Biocontrol Agent Trichoderma viride with Various Pesticides

Compatibility of Trichoderma viride with 25 pesticides was evaluated in vitro. Among six seed-treatment chemicals tested, T. viride showed a high compatibility with the insecticide Imidacloprid (7.6cm mycelial growth), followed by Mancozeb (6.3cm) and Tebuconazole (3.7cm). Contact fungicides, viz., Pencycuron and Propineb were found to be fully compatible with T. viride. Among the 10 herbicides also tested, the fungus was highly compatible with Imazathafir (9.0cm) followed by 2,4-D Sodium salt (8.9cm) and Oxyfluoforen (6.5cm) while being totally incompatible with systemic fungicides like Carbendazim, Hexaconazole, Tebuconazole and Propiconazole

Characterization of calcium and magnesium binding domains of human 5-lipoxygenase

Two calcium binding sites, separated by about 9.3 Å, present in the loops that connect the β-sheets of N-terminal domain contain the ligating residues F14, A15, G16, D79, and D18, D19, L76, respectively. Magnesium is found to bind in regions, which are marginally different owing to the disparity in the ionic radii of Ca<SUP>2+</SUP> and Mg<SUP>2+</SUP>. The entropy analysis on the loops of 5-lipoxygenase, implementing the wormlike chain model, explains that the N-terminal β-barrel is well suited to accommodate calcium binding sites. The large buried side chain area of W102 (compared to W13 and W75) and comparatively smaller fraction of side chain exposed to polar atoms corroborate the calcium induced higher affinity to phosphatidylcholine (PC). However, W80 lying in close proximity of the calcium binding sites is expected to have considerable PC affinity but negligible calcium induced effect on PC binding

IN-VITRO EVALUATION OF FUNGICIDES, BIOCONTROL AGENTS AND PLANT EXTRACTS AGAINST RICE SHEATH BLIGHT PATHOGEN RHIZOCTONIA SOLANI

ABSTRACT: Of the fourteen fungicides of different groups evaluated in-vitro against Rhizoctonia solani, Metalaxyl (0.1%), Mancozeb (0.1%), Tricyclazole (0.1%), Thiophenate methyl (0.1%), Carbendizm+ Mancozeb (0.1%) were proved to be most effective in inhibiting the growth of the fungus. Among the bio-agents screened, Trichoderma viride was most effective in restricting the growth of Rhizoctonia solani followed by Penicillium notatum where as Aspergillus niger was proved least effective. Among the thirteen plant extracts evaluated garlic extract (10%) was most effective in inhibiting the growth of fungus followed by calotropis (10%). Datura leaf extract (10%) was found to be least effective in inhibiting the growth of Rhizoctonia solani

Structural and conformational changes concomitant with the E1–E2 transition in H<SUP>+</SUP>K<SUP>+</SUP>-ATPase: a comparative protein modeling study

Comparative modeling studies on conserved regions of the gastric H+K+-ATPase reveal that the E1–E2 conformational transition induces significant tertiary structural changes while conserving the secondary structure. The residues 516–530 of the cytoplasmic domain and TM10 within the transmembrane (TM) regions undergo maximum tertiary structural changes. The luminal regions exhibit comparatively lesser tertiary structural deviations. Residues 249–304 show maximum secondary structural deviation in the conformational transition. The Cys-815 and Cys-323 residues involved in inhibitor binding are found to have smaller buried side chain areas in the E1 conformation compared to E2. Retention of activity correlates well with the buried side chain area when selected amino acid residues in TM6 are mutated using modeling techniques with bulkier amino acid residues. Conformational specificity for ion binding is corroborated with the fraction of side chains exposed to polar atoms of the residues E345, D826, V340, A341, V343, and E822