PHYTOCHEMICAL AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF EXTRACT OF PORTULACA QUADRIFIDA LINN.

Objective: Portulaca quadrifida L. is an herbal medicinal plant known for its therapeutics values in urinary and inflammatory disorders. Leaves areuseful in dysentery; the plant can also act as an antihelminthic. The current study dealt to provide details information about Portulaca quadrifida L.including pharmacognostic and phytochemical analysis.Methods: Current investigation involve quality control characterization of plant Portulaca quadrifida L. The plant extract evaluated for phytochemicaland chromatographic analysis. HPLC fingerprint was carried out, which can be used for correct identification of the plant.Results: The plant extract contains alkaloids, tannins, terpenoid and steroid. The present study provides evidence that solvent extract of Portulacaquadrifida L. contain medicinally important bioactive compounds.Conclusion: The present study provides evidence that solvent extract of plant contains medicinally important bioactive compounds and this justifiesthe use of plant species as traditional medicine for treatment of various diseases.Keywords: Portulaca quadrifida L., Phytochemical, Medicinal plant, High-performance liquid chromatography

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â

ANTIMICROBIAL AND PHYTOCHEMICAL EVALUATION OF CISSUS QUADRANGULARIS L.

The present investigation involves antimicrobial and phytochemical evaluation of Cissus quadrangularis L. The antibacterial activity of Cissus quadrangularis was performed using disk diffusion method. The Results of study proved prompt efficacy of herbal extract against S. aureus and E. coli. The concentration dependent antibacterial activity of extract was observed against both organisms. Study also involves phytochemical investigation of herbal extract using HPTLC, IR and UV-Visible spectrophotometer. The result of study indicated that the methanolic extract possessed most potent antibacterial activity as compared to other extract. The antibacterial activity increases with the concentration and results indicated that the diameters of zone inhibition of the extract were comparable with the standard drug. The antimicrobial potential of plant extract may be attributed to the presence of specific phytoconstituents

PHYTOCHEMICAL STANDARDIZATION AND ANTIOXIDANT POTENTIAL OF AYURVEDA FORMULATION DARVYADI RASKRIYA

The study represents phytochemical standardization and antioxidant potential of ayurveda formulation Darvyadi Raskriya. The study involves development of high-performance thin-layer chromatographic (HPTLC) method for analysis of formulation. The study utilizes analysis of glycyrrhizin in formulation which is the phytoconstituent of Glycyrrhiza glabra one of the component of formulation. The sample in ethanol was applied on aluminium TLC plates using Linomat 5 spray (CAMAG). Linear ascending development was performed in twin trough glass chamber saturated with mobile phase. The mobile phase consisted of ethyl acetate-methanol-formic acid (10:5:1 v/v/v). The spectrodensitometric detection was performed at the wavelength of 254 nm. The regression analysis was found to be linear with r2=0.997 in the concentration range 5-25 ppm. The antioxidant activity of formulation was also found to be significant as compared to control. Keywords: Standardization, Glycyrrhiza glabra, Glycyrrhizin, HPTLC, Antioxidant