4 research outputs found
Plasma Vitamin D levels in correlation with circulatory proteins could be a potential biomarker tool for pulmonary tuberculosis and treatment monitoring
Background: Tuberculosis (TB), a life-threatening immune challenging disease to the global human community
has to be diagnosed earlier and eliminated in the upcoming era. Vitamin D, a fat-soluble micronutrient, mainly
from epidermal cells of the skin and a few dietary sources, is associated with the immune system in various
disease management. Therefore, a better understanding of vitamin D metabolism and immune function in
tuberculosis should be studied for the consideration of biomarkers.
Methods: The study consist of Pulmonary Tuberculosis (PTB) patients (n = 32) at two-time points: Baseline (PTB
BL) and after 6 months of anti-TB treatment (ATT) (PTB PT), latently Mtb infected (IFNγ + ) group (n = 32) and a
non-LTB healthy control (IFNγ-) group (n = 32). Vitamin D levels were measured using High-performance liquid
chromatography (HPLC). The cytokine data from the same participants assayed by ELISA from our earlier in�vestigations were used to correlate it with serum Vitamin D levels.
Results: The assayed serum Vitamin D levels between the groups showed significantly lowered levels in PTB BL
when compared with IFNγ + and IFNγ- groups. And, the Vitamin D levels in the PTB group after ATT were
significantly lower than the baseline levels. The Vitamin D data were compared with pro- and anti-inflammatory
cytokines and adipokines levels by performing a principal component regression analysis. Based on the PC scores,
the study group showed distinct clusters for the TB group and control group. And, the correlation analysis be�tween the study group and immunological indices showed significant correlations. Vitamin D significantly
correlated with IFNγ, TNFα, IL17A, IL-4 and Resistin in the TB group, whereas IL-6 and G-CSF in the control
group.
Conclusion: The baseline measurement of Vitamin D levels was significantly decreased in the PTB group when
compared with IFNγ + and IFNγ- groups showing the importance of Vitamin D as a preventive factor against the
TB disease progression. The six-month post-treatment of TB showed a further decrease in Vitamin D levels in
PTB. The significantly correlated immunological indices with Vitamin D levels are the biomarker profile that
could predict TB
Prevalence of vitamin D deficiency and its associated factors among the urban elderly population in Hyderabad metropolitan city, South India
Background: Deficiency of vitamin D has been associated with various health conditions. However, vitamin D deficiency (VDD) and factors associated with VDD are not well studied, especially among the urban elderly population of India. Aim: To assess the prevalence of VDD and its associated factors among the urban free-living elderly population in Hyderabad. Subjects and methods: A community-based cross-sectional study was conducted among 298 urban elderly (≥60 years) by adapting a random sampling procedure. Demographic particulars were collected. Blood pressure and anthropometric measurements were recorded using standard equipment. Fasting glucose, lipid profile and 25-hydroxy vitamin D [25(OH) D] were estimated in plasma samples. Results: The mean ± SE plasma vitamin D and the prevalence of VDD among the urban elderly population were 19.3 ± 0.54 (ng/ml) and 56.3%, respectively. The prevalence of VDD was significantly associated with education, high body mass index (BMI), hypertension (HT) and metabolic syndrome (MS). Multiple logistic regression analysis revealed HT as a significant predictor of vitamin D deficiency and the risk of VDD was double among the elderly with hypertension. Conclusions: The prevalence of VDD was high among the urban elderly population in the south Indian city of Hyderabad. High BMI, MS, HT and education are significant associated factors of VDD