Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-07-07T17:28:35Z No. of bitstreams: 1 Maciel MC Tityus serrulatus....pdf: 958216 bytes, checksum: cac508f5fee8daa8738adf84e70f79ff (MD5)Made available in DSpace on 2014-07-07T17:28:35Z (GMT). No. of bitstreams: 1 Maciel MC Tityus serrulatus....pdf: 958216 bytes, checksum: cac508f5fee8daa8738adf84e70f79ff (MD5) Previous issue date: 2014Universidade Federal do Maranhão. Laboratorio de Imunofisiologia. Departamento de Patologia. Centro de Ciências Biologicas e da Saúde. São Luís, MA, BrasilUniversidade Federal do Maranhão. Laboratorio de Imunofisiologia. Departamento de Patologia. Centro de Ciências Biologicas e da Saúde. São Luís, MA, BrasilUniversidade Federal do Maranhão. Laboratorio de Imunofisiologia. Departamento de Patologia. Centro de Ciências Biologicas e da Saúde. São Luís, MA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Estadual de Londrina. Laborat orio de Imunofarmacologia. Departamento de Ciências Patol ogicas. Centro de Ciências Biol ogicas. Londrina, PR, BrasilUniversidade Federal do Maranhão. Laboratorio de Imunofisiologia. Departamento de Patologia. Centro de Ciências Biologicas e da Saúde. São Luís, MA, BrasilTityus serrulatus venom (Tsv) modifies the behavior of immune cells and induces the production of inflammatory and anti-inflammatory cytokines; such action may interfere with physiological or pathological states. Because sepsis is characterized as an inflammatory disorder, the aim of present study was to investigate the effect of a non-lethal dose of Tsv in mice submitted to a polymicrobial infection by cecal ligation and puncture (CLP) model. The parameters evaluated were survival index, cellularity on lymphoid organs, peritoneal cavity and brochoalveolar space, production of IL-10, IL-12, IL-6, TNF-a, IFN-g and MCP-1, pulmonary inflammation and oxidative burst. The results demonstrated that in sharp contrast to CLP group in which sepsis was lethal in a 24 h period all mice pretreated with Tsv survived even 60 h after CLP. Lung inflammation, another hallmark of CLP group, was also dramatically down regulated in Tsv/CLP group. Despite pretreatment with Tsv did not reduce the inflammatory serum cytokines when compared to CLP group; there was an increase in IL-10. In conclusion, subcutaneous Tsv administration 6 h before CLP was able to control the harmful effects of sepsis (lethality and lung inflammation). We suggest that both systemic IL-10 and oxidative burst are involved in this effec