Clinical and pathological aspects in broilers (Gallus gallus domesticus) infected experimentally with Eimeria acervulina Tyzzer, 1929 sporulated oocysts

No presente estudo, sinais clínicos e alterações patológicas foram avaliados por 30 dias em frangos de corte, linhagem Cobb, machos, com dez dias de idade, infectados com Eimeria acervulina. Foram utilizados 192 animais distribuídos em 3 grupos: grupo A inoculado com 1x10(6) oocistos esporulados; grupo B inoculado com 1x10(5) oocistos esporulados; grupo C inoculado com água destilada. Os sinais clínicos observados foram anorexia, diarréia e apatia. As alterações patológicas macroscópicas observadas foram: enterite, hiperemia seguido de congestão intestinal, excesso de exsudato mucoso no lúmen do intestino delgado, palidez e desidratação muscular, alto acúmulo de bile na vesícula biliar e deposição de gordura hepática. A atrofia de vilosidades e alta presença de células inflamatórias foram as alterações microscópicas observadas no epitélio intestinal. Na análise histopatológica do fígado observaram-se infiltrados inflamatórios e deposição de gordura. Os resultados demonstraram que frangos de corte infectados experimentalmente com E. acervulina apresentam progressivas lesões intestinais de intensidade variável e que essas anormalidades são as principais causas de redução no desenvolvimento da ave.In the present study, clinical signs and pathological changes were evaluated during 30 days in broilers, lineage Cobb, males, with ten days old, infected with Eimeria acervulina. Were used 192 animals distributed in 3 groups: group A inoculated with 1x10(6) sporulated oocysts; group B inoculated with 1x10(5) sporulated oocysts; group C inoculated with distilled water. The clinical signs observed were anorexia, diarrhea and apathy. The pathological macroscopic changes observed were: enteritis, hyperemia with subsequent intestinal congestion, high concentration of mucus exudates in the lumen of the small intestine, pale and dehydration muscle, gall bladder full of liquid and deposition of hepatic fat. The villous atrophy and high presence of inflammatory cells were the microscopic changes observed in the gut epithelium. In histopathologic analysis of liver observed inflammatory cells infiltrated and fat deposition. The results demonstrate that broilers experimentally infected with E. acervulina presented progressive intestinal lesions of variable intensity and that these abnormalities are the main cause of reduction of bird performance

A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test.

To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA