Características silviculturais e potencial de uso da espécie arbórea Licania macrophilla Benth (anoerá/anauerá).

Licania macrophylla Benth (anoerá ou anuerá), família Chrysobalanaceae, é árvore que ocorre no estuário do Rio Amazonas, com porte de 17 a 28m de altura, desprovida de sapopemas, fuste retilíneo e madeira de cor avermelhada, com potencial para uso na construção civil e fabricação de móveis. Sua casca é utilizada na medicina caseira e na fabricação de fitoterápicos.bitstream/item/95969/1/CPAF-AP-2005-caracteristicas-licania.pd

A promoter region that controls basal and elicitor-inducible expression levels of the NADPH: cytochrome P450 reductase gene (Cpr) from Catharanthus roseus binds nuclear factor GT-1

NADPH:cytochrome P450 reductase (CPR) is essential for the activation of cytochrome P450 enzymes, which are involved in a wide variety of metabolic pathways in plants, including those related to defence responses. In the subtropical plant Catharanthus roseus several cytochrome P450 enzymes operate in the biosynthesis of defence-related terpenoid indole alkaloids (TIAs). In agreement with the importance of CPR in defence, Cpr mRNA levels in C. roseus were found to be enhanced by fungal elicitor preparations that also induce TIA biosynthesis and P450 gene expression. Here we describe the isolation of a C. roseus genomic DNA clone covering the 5¢ part of the Cpr gene and 1.6-kb of upstream sequences. Mapping of the transcription start site showed the untranslated leader sequence is approximately 280 bp long. To study the control of gene expression by the Cpr promoter, transcriptional fusions between Cpr promoter fragments and the gusA reporter gene were generated and their expression was analyzed in stably transformed tobacco plants. The Cpr promoter fragment extending from )1510 to )8, with respect to the ATG start codon, conferred basal and elicitor-inducible expression on the gusA reporter gene, strongly indicating that the Cpr gene of C. roseus is indeed controlled by this promoter region. Progressive deletion from the 5¢ end of the promoter to position )632 had little e ect on gusA expression. However, deletion to position )366 resulted in a complete loss of basal activity and largely eliminated elicitor-induced expression, indicating that the region from )632 to )366 contains the main transcription-enhancing cis-regulatory sequences. Electrophoretic mobility shift assays with tobacco nuclear extracts showed that binding sites for nuclear factor GT-1 are redundant in the Cpr promoter, but absent from the downstream part of the leader sequence. The presence of strong GT-1 binding sites in the main enhancer region ()632 to )366), is suggestive of a functional role for this factor in basal expression and elicitor responsiveness of the Cpr promoter.info:eu-repo/semantics/publishedVersio

A promoter region that controls basal and elicitor-inducible expression levels of the NADPH: cytochrome P450 reductase gene (Cpr) from Catharanthus roseus binds nuclear factor GT-1

NADPH:cytochrome P450 reductase (CPR) is essential for the activation of cytochrome P450 enzymes, which are involved in a wide variety of metabolic pathways in plants, including those related to defence responses. In the subtropical plant Catharanthus roseus several cytochrome P450 enzymes operate in the biosynthesis of defence-related terpenoid indole alkaloids (TIAs). In agreement with the importance of CPR in defence, Cpr mRNA levels in C. roseus were found to be enhanced by fungal elicitor preparations that also induce TIA biosynthesis and P450 gene expression. Here we describe the isolation of a C. roseus genomic DNA clone covering the 5¢ part of the Cpr gene and 1.6-kb of upstream sequences. Mapping of the transcription start site showed the untranslated leader sequence is approximately 280 bp long. To study the control of gene expression by the Cpr promoter, transcriptional fusions between Cpr promoter fragments and the gusA reporter gene were generated and their expression was analyzed in stably transformed tobacco plants. The Cpr promoter fragment extending from )1510 to )8, with respect to the ATG start codon, conferred basal and elicitor-inducible expression on the gusA reporter gene, strongly indicating that the Cpr gene of C. roseus is indeed controlled by this promoter region. Progressive deletion from the 5¢ end of the promoter to position )632 had little e ect on gusA expression. However, deletion to position )366 resulted in a complete loss of basal activity and largely eliminated elicitor-induced expression, indicating that the region from )632 to )366 contains the main transcription-enhancing cis-regulatory sequences. Electrophoretic mobility shift assays with tobacco nuclear extracts showed that binding sites for nuclear factor GT-1 are redundant in the Cpr promoter, but absent from the downstream part of the leader sequence. The presence of strong GT-1 binding sites in the main enhancer region ()632 to )366), is suggestive of a functional role for this factor in basal expression and elicitor responsiveness of the Cpr promoter.info:eu-repo/semantics/publishedVersio

Composição florística e estrutura de floresta em várzea alta estuarina amazônica.

O presente estudo foi desenvolvido objetivando avaliar a composição florística e a estrutura de floresta de várzea alta do estuário amazônico. Uma parcela amostral de 1,0 ha, divida em subparcelas de 20 x 50 m, foi instalada no Bailique/AP, furo do Mazagão/PA e medidos os indivíduos com DAP..

Potencial de utilização madeireira de espécies florestais de várzea no município de Mazagão no estado do Amapá.

O presente estudo foi desenvolvido objetivando avaliar o potencial de utilização madeireira das espécies de várzea estuarina amazônica, no município de Mazagão, estado do Amapá, e estimar o estoque de madeira das espécies exploradas. Para avaliar o potencial de utilização madeireira, aplicou-se questionário para levantamento de informações socioeconômicas dos proprietários das pequenas serrarias existentes no rio Mazagão, rio Mutuacá e rio Ajudante, tributários do rio Amazonas. Para estimar o estoque de madeira existente das espécies exploradas, foram instaladas cinco parcelas amostrais de 1,0 ha, divididas em subparcelas de 20x50 m. Pelas respostas nos questionários, observou-se que as espécies mais serradas na área são: andiroba (Carapa guianensis Aubl.), pracuúba (Mora paraensis Ducke), pau-mulato (Callycophyllum spruceanum Benth.), macacaúba (Platymiscium filipes Benth.), virola (Virola surinamensis (Rol. Ex Rottb) Wab) e anani (Symphonia globulifera L.). O inventário fitossociológico mostrou que o estoque de madeira das espécies exploradas é satisfatório, com 71,8 árvores/ha com DAP entre 20 e 40 cm e 28,2 árvores/ha com DAP > 40 cm. Atualmente, em média, em cada serraria são produzidas, por dia, 20 dúzias de tábuas e pernamancas, pelo beneficiamento de 7 a 8 toras de madeira, obtidas com derrubada de duas árvores

Frontal-subcortical behaviors during Alzheimer's disease in individuals with Down syndrome.

There is evidence that frontal-subcortical circuits play an important role in the initial presentation of dementia in Down syndrome (DS), including changes in behavior, a decline in working memory and executive dysfunction. We evaluated 92 individuals with DS (≥30 years of age), divided into 3 groups by diagnosis-stable cognition, prodromal dementia, and Alzheimer's disease. Each individual was evaluated with an executive protocol developed for people with intellectual disabilities and was rated for behaviors related to frontal lobe dysfunction (disinhibition, executive dysfunction, and apathy) by an informant using the Frontal Systems Behavior Scale. Informant-reported behaviors related to frontal lobe dysfunction were found to correlate negatively with executive function performance. Disinhibition and executive dysfunction were associated with the clinical stage of dementia. The odds of having Alzheimer's disease increased in parallel with increases in the domain and total Frontal Systems Behavior Scale scores (p ≤ 0.5). Disinhibition, executive dysfunction and apathy should be taken into consideration during the clinical evaluation of adults with DS, and future studies should consider the intersection of neuropathology, brain connectivity, and behavior

Indicadores para a avaliação ambiental em pesque-pagues nas dimensões ecologia da paisagem e qualidade da água.

O objetivo do presente trabalho é recomendar indicadores que favoreçam melhorias no desempenho ambiental em uma série de pesque-pagues localizados próximos à cidade de Campinas, SP. Além disso, é apresentada uma proposta para a validação do conjunto de indicadores para análise específica dos pesque-pagues, com ênfase nas dimensões de avaliação ambiental Ecologia da Paisagem e Qualidade da Água.bitstream/CNPMA/7432/1/boletim_41.pd